Reaction Conditions for the Production of<i>γ</i>-Cyclodextrin by Cyclodextrin Glucanotransferase from<i>Brevibacterium</i>sp. No. 9605

Mori, Shigeharu; Goto, Masataka; Mase, Tamio; Matsuura, Akira; Oya, Takaichi; Kitahata, Sumio

doi:10.1271/bbb.59.1012

Cited by 27 publications

(16 citation statements)

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“…Moreover, the effect of ethanol on starchy materials from various sources was similar. Mori et al [18], using For the selected concentration of ethanol, 10% (v/v), the results for the variation in enzyme concentration and sampling at 12-h intervals confirmed what had already been observed in Fig. 2.…”

Section: Enzyme and Ethanol Concentration In The Reaction Mediumsupporting

confidence: 82%

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Production of Cyclodextrins from Cornstarch Granules in a Sequential Batch Mode and in the Presence of Ethanol

Calsavara

Cunha

Balbino

et al. 2011

Appl Biochem Biotechnol

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The influence of Toruzyme® cyclomaltodextrin glucanotransferase concentration and the presence of ethanol have been studied for the production of α-, β-, and γ-cyclodextrins (CDs) from 15% (w/v) cornstarch, at 65 °C and pH 6, with the aim of increasing CD yield. The selected concentrations for a single batch reactor were 10% (v/v) ethanol and 0.1% (v/v) enzyme, yielding after 12 h, 37% total CDs, of which 52.2% was α-CD, 38.8% β-CD, and 9.0% γ-CD. The enzyme specific activities per unit mass of protein for producing α-, β-, and γ-CD were 37.25, 19.61, and 8.63 U mg(-1), respectively. Total CD yield per milliliter of enzyme was 55 g. To increase CD yield per enzyme charge and thus reduce costs, the production of CDs was tested with two sequential batches in which a single enzyme charge was used. At the end of the first batch, the enzyme was adsorbed either on 65 °C pretreated starch granules or on raw starch, and a second batch was run with this material. The best result, in this case, was obtained for pretreated starch, increasing total CD produced by 57.4%, with 53.2% α-CD, 36.1% β-CD, and 10.7% γ-CD. CD yield per milliliter of enzyme was then 87 g.

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Section: Enzyme and Ethanol Concentration In The Reaction Mediumsupporting

confidence: 82%

“…The polar groups contained in the solvent may make direct molecular effects on biocatalyst activity more likely. Mori et al [18] supposed that ethanol might change the steric structures of the starch and (or) enzyme so as to repress the formation of β-CD.…”

Section: Enzyme and Ethanol Concentration In The Reaction Mediummentioning

confidence: 99%

Production of Cyclodextrins from Cornstarch Granules in a Sequential Batch Mode and in the Presence of Ethanol

Calsavara

Cunha

Balbino

et al. 2011

Appl Biochem Biotechnol

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“…These results once more confirmed the preference of CGTase for high molecular mass substrates. The lower cyclodextrin yield from starch hydrolysates may also be due to the inhibitory effect of maltose and glucose formed by the action of a-amylase (Mori et al 1995;Rendleman 1996).…”

Section: Resultsmentioning

confidence: 99%

Effect of different substrates and their preliminary treatment on cyclodextrin production

Pishtiyski

Zhekova

2005

World J Microbiol Biotechnol

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Various kinds of substrates were tested for cyclodextrin production with cyclodextrin glucanotransferase (CGTase) from Bacillus megaterium. The enzyme formed cyclodextrin from different kinds of starch, dextrins, amylose, and amylopectin. However, the highest degree of conversion was obtained from starch. Corn starch appeared to be the best substrate -the cyclodextrin yield was 50.9%. The effect of molecular mass and preliminary treatment of starch with a-amylase on the CD yield was investigated. It was proved that CGTase preferred native starch with high molecular mass and low dextrose equivalent. The preliminary treatment with aamylase occurred to be inefficient and unnecessary since it did not lead to an increase in the CD yield. Some of the substrates were treated with pullulanase. The effect of debranching was highest in the case of corn starch: the cyclodextrin yield increased by 10%.

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“…Using CGTase from Brevibacterium sp no. 9605, Mori et al (10,11) have observed 1% lower conversion when using corn starch than when using sweet potato starch. Similar results were observed by Goel and Nene (12) who obtained a better CD conversion using CGTase from Bacillus firmus in cassava starch than in corn starch.…”

Section: Effect Of Kind Of Starch On CD Formationmentioning

confidence: 98%

“…On the other hand, addition of selective complexant agents and organic solvents to the reaction mixture has also shown to significantly influence the ratios of α-, β-, and γ-CDs and their yields. Many works have shown that different substrates can determine the kind of product obtained from enzymatic reaction CDs (9)(10)(11)(12)(13)(14)(15)(16).…”

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confidence: 99%

Production of Cyclodextrins by CGTase from Bacillus clausii Using Different Starches as Substrates

Alves-Prado

Carneiro

Pavezzi

et al. 2007

Appl Biochem Biotechnol

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Cyclodextrins (CDs) are cyclic oligasaccharides composed by D-glucose monomers joined by alpha-1,4-D glicosidic linkages. The main types of CDs are alpha-, beta- and gamma-CDs consisting of cycles of six, seven, and eight glucose monomers, respectively. Their ability to form inclusion complexes is the most important characteristic, allowing their wide industrial application. The physical property of the CD-complexed compound can be altered to improve stability, volatility, solubility, or bio-availability. The cyclomaltodextrin glucanotransferase (CGTase, EC 2.4.1.19) is an enzyme capable of converting starch into CD molecules. In this work, the CGTase produced by Bacillus clausii strain E16 was used to produce CD from maltodextrin and different starches (commercial soluble starch, corn, cassava, sweet potato, and waxy corn starches) as substrates. It was observed that the substrate sources influence the kind of CD obtained and that this CGTase displays a beta-CGTase action, presenting a better conversion of soluble starch at 1.0%, of which 80% was converted in CDs. The ratio of total CD produced was 0:0.89:0.11 for alpha/beta/gamma. It was also observed that root and tuber starches were more accessible to CGTase action than seed starch under the studied conditions.

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Reaction Conditions for the Production ofγ-Cyclodextrin by Cyclodextrin Glucanotransferase fromBrevibacteriumsp. No. 9605

Cited by 27 publications

References 1 publication

Production of Cyclodextrins from Cornstarch Granules in a Sequential Batch Mode and in the Presence of Ethanol

Production of Cyclodextrins from Cornstarch Granules in a Sequential Batch Mode and in the Presence of Ethanol

Effect of different substrates and their preliminary treatment on cyclodextrin production

Production of Cyclodextrins by CGTase from Bacillus clausii Using Different Starches as Substrates

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