Reaction Mechanism between Nitric Oxide and Glutathione Mediated by Fe(III) Myoglobin

Reichenbach, Gustavo; Sabatini, Stefano; Palombari, R.; Palmerini, Carlo Alberto

doi:10.1006/niox.2001.0365

Cited by 33 publications

(27 citation statements)

References 24 publications

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“…It has been shown that metHb binds NO ⅐ and generates HbFe(III)NO, a nitrosyl complex that has formally HbFe(II)(NO ϩ ) character (50 -52), and could thus act as a nitrosating agent. The corresponding myoglobin complex MbFe(II)(NO ϩ ) has been shown to undergo NO ϩ transfer and, among others, react with thiols to generate S-nitrosothiols (53,54). Here we show that reaction of metHb with NO ⅐ leads to nitrosation of Cys-␤93.…”

Section: Discussionmentioning

confidence: 69%

Reactions of Deoxy-, Oxy-, and Methemoglobin with Nitrogen Monoxide

Herold

Röck

2003

Journal of Biological Chemistry

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The reaction between hemoglobin (Hb) and NO ⅐ has been investigated thoroughly in recent years, but its mechanism is still a matter of substantial controversy. We have carried out a systematic study of the influence of the following factors on the yield of S-nitrosohemoglobin (SNO-Hb) generated from the reaction of NO ⅐ with oxy-, deoxy-, and metHb: 1) the volumetric ratio of the protein and the NO ⅐ solutions; 2) the rate of addition of the NO ⅐ solution to the protein solution; 3) the amount of NO ⅐ added; and 4) the concentration of the phosphate buffer. Our results suggest that the highest SNO-Hb yields are mostly obtained by very slow addition of substoichiometric amounts of NO ⅐ from a diluted solution. Possible pathways of SNO-Hb formation from the reaction of NO ⅐ with oxy-, deoxy-, and metHb are described. Our data strongly suggest that, because of mixing artifacts, care should be taken to use results from in vitro experiments to draw conclusion on the mechanism of the reaction in vivo.

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Section: Discussionmentioning

confidence: 69%

Reactions of Deoxy-, Oxy-, and Methemoglobin with Nitrogen Monoxide

Herold

Röck

2003

Journal of Biological Chemistry

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“…Several authors have suggested that GSH is the prime thiol-containing target for NO in a cell (Kröncke et al, 1998;Reichenbach et al, 2001), leading to formation of nitrosoglutathione (GSNO), which in turn has been shown to enhance expression of the genes involved in differentiation of brown adipocytes (Nisoli et al, 1998). Moreover, Gaudiot et al (1998Gaudiot et al ( , 2000 showed that GSNO acting as a NO donor increases basal lipolysis in white fat cells, thus increasing the concentration of free fatty acids, which are the main fuel for IBAT thermogenesis (Trayhurn, 1979;Bukowiecki et al, 1981) and are also known to activate UCP1 (Skulachev, 1991;Winkler and Klingerbeg, 1994).…”

Section: Discussionmentioning

confidence: 99%

The effects of l-arginine and l-NAME supplementation on redox-regulation and thermogenesis in interscapular brown adipose tissue

Petrović

Korać

Buzadžić

et al. 2005

Journal of Experimental Biology

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These data suggest that nitric oxide (NO) produced by iNOS could also contribute to overall NO-associated regulation of thermogenesis in IBAT. Namely, that iNOS, i.e. NO, in correlation with enhanced thermogenesis, additionally induced IBAT hyperplasia and UCP1 level compared to that induced by low temperature. Cooperative action of decreased apoptosis accompanied by increased tissue hyperplasia and UCP1 level, observed in IBAT of cold-acclimated rats, would be a way of meeting the metabolic requirements for increased thermogenesis.

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“…(3), reactive nitrogen oxide intermediates donated as NOx) such as occuring in nitrosoglutathione production (Kroncke et al, 1998;Reichenbach et al, 2001). RNOI or S-nitrosothiols, the latter via a transnitrosylation mechanism, can S-nitrosylate cystein thiols involved in Zn 2 + complexation in zinc finger structures, leading to conformational changes and modifications of functional activities (Coffey et al, 2002;Kroncke, 2001).…”

Section: Introductionmentioning

confidence: 99%