Reaction mechanism of surfactant‐sensitized chemiluminescence of bis(2,4,6‐trichlorophyenyl) oxalate and hydrogen peroxide induced by gold nanoparticles

Liang, Shu-Xuan; Li, Haifang; Lin, Jin‐Ming

doi:10.1002/bio.1049

Cited by 18 publications

(15 citation statements)

References 26 publications

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“…After the completion of the immune response process, the chemiluminescence analysis process was based on the reaction of 3-(4-hydroxyphenyl propionate) (PHPPA) with hydrogen peroxide-urea (CH 4 N 2 O·H 2 O 2 ), catalyzed by HRP, produced fluorescence of 3-(4-hydroxyphenyl propionate) dimmer, and then, hydrogen peroxide and TCPO react and produce an active intermediate, postulated as 1,2-dioxetanedione [32], which quickly transferred energy to the fluorophore of PHPPA dimmer. Its reaction with the high energy intermediate produces the excited state product which quickly emits light.…”

Section: Optimization Of Investigation Conditionsmentioning

confidence: 99%

Highly Sensitive Chemiluminescent Analysis of Residual Bovine Serum Albumin (BSA) Based on a Pair of Specific Monoclonal Antibodies and Peroxyoxalate–glyoxaline–PHPPA Dimer Chemiluminescent System in Vaccines

Xue

Zhang

et al. 2012

Appl Biochem Biotechnol

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Enzyme-linked immunosorbent assay (ELISA), horseradish peroxidase (HRP)-catalyzed fluorescent reaction, and oxalate chemiluminescence analysis have been combined to develop a highly sensitive, simple, and rapid method for analysis of bovine serum albumin (BSA) based on a pair of specific monoclonal antibodies in vaccines. A typical "sandwich type" immunoassay was used. Reaction of 3-(4-hydroxyphenyl propionate) (PHPPA) with hydrogen peroxide-urea, catalyzed by HRP, produced fluorescence of 3-(4-hydroxyphenyl propionate) dimer, which was detected by chemiluminescence analysis with the bis(2,4,6-trichlorophenyl)oxalate (TCPO)-H(2)O(2)-glyoxaline-PHPPA dimer chemiluminescent system. This method exhibited high performance with a linear correlation between response and amount of bovine serum albumin (BSA) in the range 0.1 to 100.0 ng mL(-1) (r = 0.9988), and the detection limit was 0.03 ng mL(-1) (S/N = 3). Intra- and interassay coefficient variations were all lower than 9.0% at three concentrations (1.0, 20.0, and 80.0 ng mL(-1)). The proposed method has been used for successful analysis of the amount of residual BSA in vaccines. The results obtained compared well with those obtained by conventional colorimetric ELISA and luminol chemiluminescent ELISA.

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Section: Optimization Of Investigation Conditionsmentioning

confidence: 99%

Highly Sensitive Chemiluminescent Analysis of Residual Bovine Serum Albumin (BSA) Based on a Pair of Specific Monoclonal Antibodies and Peroxyoxalate–glyoxaline–PHPPA Dimer Chemiluminescent System in Vaccines

Xue

Zhang

et al. 2012

Appl Biochem Biotechnol

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“…Another simple method is the use of surfactants, which lead to changes in the microenvironments in which reactive intermediates take part in the chemiexcitation steps by interacting with the fluorophores. Several reports have dealt with the effects of surfactants on PO‐CL, and various effects of neutral, cationic and anionic surfactants on the CL efficiency have been documented , however, these results cannot be easily integrated into a simple system because surfactants behave in different ways depending on the reactants and the reaction conditions.…”

Section: Introductionmentioning

confidence: 99%

Peroxyoxalate chemiluminescence enhanced by oligophenylenevinylene fluorophores in the presence of various surfactants

Motoyoshiya

Takigawa

2014

Luminescence

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Abstract:The effect of several surfactants on peroxyoxalate chemiluminescence (PO-CL) using oligophenylenevinylene fluorophores was investigated. Among several oligophenylenevinylenes consisting of some stilbene units, linearly conjugated ones, such as distyrylbenzene and distyrylstilbene, effectively enhanced PO-CL efficiency. Various effects of anionic, cationic, amphoteric and non-ionic surfactants on the CL efficiency of PO-CL were determined using three oxalates and the distyrylbenzene fluorophore. Anionic and non-ionic surfactants effectively enhanced CL efficiency, in contrast to the negative effect of cationic and amphoteric surfactants.Non-ionic surfactants were also effective in CL reactions of oxalates bearing dodecyl ester groups by the hydrophobic interaction between their alkyl chains. Considering these results, the surfactants not only increase the concentrations of water-insoluble interacting species in the hydrophobic micelle cores, but also control rapid degradation of the oxalates by alkaline hydrolysis.

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“…The results of the proposed CL systems in micellar media showed that enhancement on the CL intensity could arise by the solubilization of the reagents, the electrostatic effect of the ionic surfactant, and the alteration of the pH and the microenviroment of the CL reaction. 35,36 There are some studies on analytical applications for the determination of fluorophores, proteins, and hydrogen peroxide in aqueous samples using the PO-CL reaction with the introduction of the micelles. [28][29][30][37][38][39][40][41][42][43][44] Sodium dodecyl sulfate (SDS) micellar medium was used as a carrier to avoid the rapid degradation of TCPO in water in a flow-injection analysis, [28][29][30]41 allowing the use of TCPO in the absence of organic solvent.…”

Section: Introductionmentioning

confidence: 99%

Effect of some surfactants on the chemiluminescent reactions of bis(2,4,6-trichlorophenyl)oxalate and bis(2-nitrophenyl)oxalate with hydrogen peroxide

et al. 2012

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The chemiluminescent reactions of bis(2,4,6-trichlorophenyl)oxalate (TCPO) and bis(2-nitrophenyl)oxalate (2-NPO) with hydrogen peroxide in acetonitrile/water micellar systems (anionic, cationic, and non-ionic) and γ-cyclodextrin were studied in the presence of fluoranthene or 9,10-diphenylanthracene, imidazole, and two buffer solutions, HTRIS+/TRIS and H2PO4–/HPO42–. The relative chemiluminenscence (CL) intensity is higher in the presence of the cationic (DDAB, CTAC, DODAC, and OTAC), anionic (SDS), and non-ionic (Tween 80) surfactants. In the presence of some non-ionic surfactants (Brij 35, Brij 76, and Tween 20), the CL intensity was partially quenched compared with the reaction with no surfactant. The sensitivity for hydrogen peroxide determination in the range 0.01 × 10−4to 1.0 × 10−4mol L–1, considering the slope of the calibration curves (maximum peak height of CL vs. concentration), improved with the introduction of DDAH, CTAB, and SDS in HTRIS+/TRIS buffer.

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Reaction mechanism of surfactant‐sensitized chemiluminescence of bis(2,4,6‐trichlorophyenyl) oxalate and hydrogen peroxide induced by gold nanoparticles

Cited by 18 publications

References 26 publications

Highly Sensitive Chemiluminescent Analysis of Residual Bovine Serum Albumin (BSA) Based on a Pair of Specific Monoclonal Antibodies and Peroxyoxalate–glyoxaline–PHPPA Dimer Chemiluminescent System in Vaccines

Highly Sensitive Chemiluminescent Analysis of Residual Bovine Serum Albumin (BSA) Based on a Pair of Specific Monoclonal Antibodies and Peroxyoxalate–glyoxaline–PHPPA Dimer Chemiluminescent System in Vaccines

Peroxyoxalate chemiluminescence enhanced by oligophenylenevinylene fluorophores in the presence of various surfactants

Effect of some surfactants on the chemiluminescent reactions of bis(2,4,6-trichlorophenyl)oxalate and bis(2-nitrophenyl)oxalate with hydrogen peroxide

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