Reaction of Hemomicrocirculatory Bed of Rat Liver and Changes in the Functional State of the Nitric Oxide Cycle Under the Conditions of Modeling Alcoholic Hepatitis

Mykytenko, A. О.; Yeroshenko, G. А.

doi:10.26724/2079-8334-2020-3-73-194-200

Cited by 6 publications

(2 citation statements)

References 11 publications

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“…In rat liver homogenate following biochemical parameters were determined: the activity of inducible NO synthase (iNOS) and constitutive isoforms of NO synthase (cNOS) [8], superoxide dismutase (SOD) activity [9] and catalase [10], concentration of malonic dialdehyde (MDA) [11], concentration of peroxynitrite of alkali and alkaline earth metals (ONOO -) [12], concentration of nitrites, concentration of low molecular weight nitrosothiols (S-NO) [13], concentration of sulfide anion [14] and superoxide anion radical production [15].…”

Section: Methodsmentioning

confidence: 99%

“…Total NO-synthase activity was evaluated by the increase of nitrites after incubation of 10% liver homogenate (0.2 ml) for 30 min in the incubation solution (2.5 ml of 0.1 M Tris buffer, 0.3 ml of 320 mM aqueous solution of L-arginine and 0.1 ml of 1 mM NADPH+H+ solution). To determine the activity of constitutive NOS (cNOS) 1% solution of aminoguanidine hydrochloride was used and the incubation time was extended to 60 min [8]. The activi ty of inducible NOS (iNOS) was calculated by the formula: іNOS = gNOS-сNOS.…”

Section: Methodsmentioning

confidence: 99%

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Influence of NF-κB on the development of oxidative-nitrosative stress in the liver of rats under conditions of chronic alcohol intoxication

Mykytenko¹,

Akimov²,

Yeroshenko³

et al. 2023

Ukr.Biochem.J.

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Alcohol-related liver disease is the most common cause of liver disease worldwide. The purpose of this work is the establishment of the influence of the transcription factor κB on the development of oxidative-nitrosative stress in the liver of rats under conditions of chronic alcohol intoxication. The experiments were performed on 24 male Wistar rats weighing 180-220 g. The animals were divided into 4 groups of 6 animals: control; animals, which were administered NF-κB inhibitor, namely ammonium pyrrolidinedithiocarbamate (PDTC) at a dose of 76 mg/kg 3 times a week; animals, on which we simulated alcoholic hepatitis and group of combination of alcoholic hepatitis and NF-κB inhibitor. We determined in rat liver homogenate the following biochemical parameters: the activity of NO synthase isoforms, superoxide dismutase and catalase activity, the concentration of malonic dialdehyde, the concentration of peroxynitrite, nitrites and nitrosothiols, concentration of sulfide anion and superoxide anion radical production. Chronic alcohol intoxication led to increased production of reactive oxygen and nitrogen species on the background of decreased antioxidant activity, thus intensifying lipid peroxidation in the liver. Blockade of the transcription factor κB during chronic alcohol intoxication despite an increase in antioxidant activity and decrease of reactive oxygen and nitrogen species production did not ameliorate oxidative damage to the liver. Blockade of activation of nuclear transcription factor κB in rat liver by PDTC reduced the risk of oxidative damage to hepatocytes, but did not reduce the risk of developing nitrosative damage to hepatocytes. Keywords: chronic alcohol intoxication, NF-κB, oxidative-nitrosative stress, PDTC, rat liver

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