Reaction of human non-collagenous polypeptides with coeliac disease autoantibodies

Detection and Characterisation of Anti-Endomysial Antibody in Coeliac Disease Using Human Umbilical Cord

“…However, a study using immunogold staining and electron microscopy has shown that binding sites of AEA are closely associated with fine collagenous-reticulin fibres [18], and studies of such fibres in human tissues show them to be composed of type I and III collagen [19], Alternative ly, the position of jejunal IgA deposits on the epithelial basement membrane suggests that they might be reactive against a type IV collagen. Our inhibition assays using anti collagen antibodies do not support the hypothesis of a colla genous epitope and arc therefore consistent with the find ings o f Maki et al [16]. However, these experiments are un able to exclude a collagenous nature of the epitope since the anticollagen antibodies and AEA might have different epi topes on the same collagenous macromolecule.…”

“…Our tissue adsorption results have confirmed this con clusion and importantly have found no such difference between the behaviour of antibodies detected on HUC and those detected on primate tissue. Recently, Maki et al [16] have purified the putative anti gen from fetal lung tissue, this material being able to extract anti-connective tissue antibodies from patient sera, and analysis of this material has suggested that it is non-collagenous [17]. However, a study using immunogold staining and electron microscopy has shown that binding sites of AEA are closely associated with fine collagenous-reticulin fibres [18], and studies of such fibres in human tissues show them to be composed of type I and III collagen [19], Alternative ly, the position of jejunal IgA deposits on the epithelial basement membrane suggests that they might be reactive against a type IV collagen.…”

Antibodies in Relation to Gluten Intake

“…extracellular matrix of most tissues [20]. We identified and purified extracellular matrix non-collagenous protein molecules in human fetal lung tissue which bound specifically to serum reticulin and endomysial IgA from patients with coeliac disease [21]. We further showed the auto-antigen to be expressed by human fibroblasts [22], and human umbilical cord Wharton's jelly-derived fibroblasts could be used as antigen in a novel whole-cell enzyme-linked immunosorbent assay (ELISA) [13].…”

Celiac Disease: a model autoimmune disease with gene therapy applications

“…21,22 Although the four key factors (HLA alleles, triggering antigen, presence of infiltrating reactive T cells and a ready availability of tissue) to perform a proper study of the T-cell adaptive immune response were at hand and well known already several years ago an essential 'ingredient' in the cooking pot of CD was missing ( Figure 1). For many years it had been reported the presence of an autoantibody response in CD which was defined as antireticulin and then antiendomysium (EMA) [23][24][25] to characterize an 'ill'-defined reactivity to extracellular, matrix component of the small intestine. This reactivity was not only observed in small intestine, but also in other sites and importantly in tissues from other species indicating that the 'antigen or antigens' was widely distributed and well preserved among different species.…”