Kumluca has an important place in terms of greenhouse vegetable cultivation. Melon is among one of the main vegetables grown in the district on about 3200 decare of land. Various diseases cause decrease in the yield and quality of melons grown undercover in Türkiye. Among them, Fusarium wilt and gummy stem blight diseases especially have caused significant losses in recent years. In this research, the incidence and severity of the root and stem rot disease in Kumluca were determined by surveys made in 72 melon greenhouses in this area. Plant and soil samples were taken to the laboratory and isolations were made. As a result, Fusarium oxysporum and Didymella bryoniae were the most frequently isolated pathogens from the plant samples, followed by other Fusarium species. Fungi with the highest isolation frequency from the soil samples were Fusarium spp., Rhizoctonia solani and Macrophomina phaseolina. In the pathogenicity test, F. oxysporum, F. solani, F. verticillioides, M. phaseolina and R. solani isolates caused severe symptoms on melon seedlings. Virulence of the F. oxysporum isolates on different cucurbit species was also investigated and it was determined that they caused severe wilting on melon and watermelon seedlings, while symptoms on squash and cucumber were moderate or slight. Additionally, reactions of five melon cultivars (Yusufbey, Çıtırex, Niovi, Ferdevs and Memory) commonly grown in the region against D. bryoniae were investigated using randomly selected four pathogen isolates. All the cultivars were susceptible to the disease.