Reaction of picrate with creatinine and cepha antibiotics.

Kroll, Martin H.; Hagengruber, C; Elin, R J

doi:10.1093/clinchem/30.10.1664

Cited by 23 publications

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“…The primary analytical method of measurement combines creatinine with picric acid to produce a colorimetric compound. This reaction is affected by interference from endogenous and exogenous substances common in many patient populations, such as glucose, bilirubin, plasma proteins, and antibiotics . Furthermore, serum creatinine is affected by the large biological variability associated with sex, age, ethnicity, and muscle mass.…”

Section: Accuracy and Precision Of Gfr Estimating Equations Utilizingmentioning

confidence: 99%

“…This reaction is affected by interference from endogenous and exogenous substances common in many patient populations, such as glucose, bilirubin, plasma proteins, and antibiotics. 5,6 Furthermore, serum creatinine is affected by the large biological variability associated with sex, age, ethnicity, and muscle mass. As shown in Table 1, several eGFR formulas to correct for these confounding factors in serum creatinine measurement, such as Cockcroft-Gault, Modification of Diet in Renal Disease (MDRD), and Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI), have been developed.…”

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confidence: 99%

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Cystatin C Is a More Reliable Biomarker for Determining eGFR to Support Drug Development Studies

Kar

Paglialunga

Islam

2018

The Journal of Clinical Pharma

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Glomerular filtration rate (GFR) is routinely used as a surrogate endpoint for the development of investigational drugs in clinical trials. GFR and staging of chronic kidney disease are typically assessed by measuring the concentration of endogenous serum biomarkers such as albumin and creatinine. However, creatinine is subject to high biological variability, and levels of creatinine do not rise until nearly 50% of kidney function is damaged, leading to inaccurate chronic kidney disease staging and false negatives. A newer biomarker for GFR, cystatin C, has been shown to be subject to less biological interference and more sensitive to early declines in kidney function. Cystatin C has also been shown to outperform creatinine as an indicator of true GFR and to add information about the occurrence of acute kidney injury. Comparison studies of cystatin C and creatinine continue to demonstrate its increased accuracy and sensitivity for changes in true GFR. While challenges remain for use of cystatin C, international agencies and working groups continue to validate cystatin C as a biomarker and accompanying GFR estimating equations for diagnostic and drug development use. In this review, we summarize these comparison studies, regulatory and industry guidelines, and clinical trial case studies for use of cystatin C in drug development.

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Section: Accuracy and Precision Of Gfr Estimating Equations Utilizingmentioning

confidence: 99%

mentioning

confidence: 99%

Cystatin C Is a More Reliable Biomarker for Determining eGFR to Support Drug Development Studies

Kar

Paglialunga

Islam

2018

The Journal of Clinical Pharma

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“…Para investigar a interferência das cefalosporinas nos valores de creatinina, a maioria dos estudos adotou metodologias estritamente in vitro e abordagens recentes são escassas (LETELLIER; DESJARLAIS, 1985;SWAIN;BRIGGS, 1977;HAGENGRUBER;ELIN, 1984;JIANG et al, 2016). O registro mais atualizado na literatura trata-se de um relato de caso que mensurou concentrações falsamente aumentadas de creatinina sérica, em um paciente tratado com cefoxitina, quando o analito foi dosado por ensaios químicos baseados no método de Jaffé (WANG et al, 2021).…”

Section: Introductionunclassified

Interferência in Vitro Da Administração De Cefalosporinas Nos Valores De Creatinina Sérica Em Pacientes Hospitalizados

Araújo,

Silva,

Lima

et al. 2024

Rev. Interfaces

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As cefalosporinas são antibióticos utilizados no tratamento de infecções por bactérias Gram-negativas e Gram-positivas em ambiente hospitalar e ambulatorial. Assim, o objetivo principal deste trabalho consistiu em avaliar o impacto das cefalosporinas nos níveis de creatinina sérica em pacientes de um hospital em Natal, Brasil. Para isso, um estudo observacional, prospectivo, transversal e analítico foi idealizado, submetido e aprovado pelo Comitê de Ética em Pesquisa da instituição. Foram avaliados pacientes internados entre maio de 2019 e março de 2020, maiores de idade e em uso de medicação, enquanto pacientes críticos, grávidas ou em terapia antineoplásica ou imunossupressora foram excluídos. Na admissão, coletaram-se dados clínicos, prescrições medicamentosas e amostras de sangue para avaliação da creatinina sérica pela reação de Jaffé. Os resultados foram submetidos a análise estatística e in vitro. Aproximadamente, 10% dos pacientes estavam usando cefalosporinas, o que pode justificar a elevação nos níveis de creatinina sérica. Conforme o modelo de regressão múltipla, a administração de cefalosporinas aumentou os níveis de creatinina em 0,61 mg/dL (IC 95% 0,06 -1,15) e a ceftriaxona foi especificamente associada a um aumento de 1,0 mg/dL (IC 95% 0,32 -1,69). Ademais, a análise in vitro revelou um aumento de 8,3% nos níveis de creatinina sérica na presença de ceftriaxona, sugerindo que esse parâmetro, avaliado pelo método de Jaffé, pode estar falsamente elevado em pacientes hospitalizados que recebem cefalosporinas. Portanto, o estudo contribui com o estabelecimento de correlações clínicas entre o uso de cefalosporinas e alterações laboratoriais, cujos achados são escassos na literatura.

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Deproteinization of serum: Another best approach to eliminate all forms of bilirubin interference on serum creatinine by the kinetic Jaffe reaction

Lolekha

Jaruthunyaluck

Srisawasdi

2001

Clinical Laboratory Analysis

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The negative interference of conjugated, unconjugated, and delta bilirubin on patient serum creatinine determined by the kinetic Jaffe reaction is the unresolved problem. We compared bilirubin interference on thirty patients' serum creatinine obtained from four analyzers, with and without deprotenization before the Jaffe reaction, to the Vitros dry enzymatic method. We found significant negative interference from bilirubin on serum creatinine in all samples directly applied to four wet chemical methods, except the one incorporated with serum blank rate. The negative interferences linearly related to bilirubin concentration. However, bilirubin did not interfere on serum creatinine obtained from all wet chemical methods incorporated with deproteinization process before the reaction. We conclude that deproteinized serum before the reaction is the best approach to eliminate all forms of bilirubin interference on serum creatinine determined by the kinetic Jaffe reaction.

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Reaction of picrate with creatinine and cepha antibiotics.

Cited by 23 publications

References 0 publications

Cystatin C Is a More Reliable Biomarker for Determining eGFR to Support Drug Development Studies

Cystatin C Is a More Reliable Biomarker for Determining eGFR to Support Drug Development Studies

Interferência in Vitro Da Administração De Cefalosporinas Nos Valores De Creatinina Sérica Em Pacientes Hospitalizados

Deproteinization of serum: Another best approach to eliminate all forms of bilirubin interference on serum creatinine by the kinetic Jaffe reaction

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