Reaction of Rape and Ethiopian Mustard Selections to Blackrot and Turnip Mosaic Virus (Tumv) in Zambia

Mingochi, D. S.; Jensen, Andrew S.

doi:10.17660/actahortic.1988.218.35

Cited by 5 publications

(2 citation statements)

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“…Other than Portugal, where it is native (Miguel, 1957), tronchuda is cultivated in contiguous areas with Portuguese influence (Msikita and Skirvin, 1989). Tronchuda has wide adaptation, producing 1.6 t seed/ha in a subtropical climate (Msikita and Mnzava, 1988); it has resistance to turnip mosaic virus, an ubiquitous pathogen (Asian Vegetable Research Center,198.5) of Brassica and other plant species (Mingochi and Jensen, 1988).…”

mentioning

confidence: 99%

Propagation of Tronchuda (Brassica oleracea var. tronchuda Bailey) from Cuttings

Msikita¹,

Wilkinson²,

Skirvin³

1992

HortSci

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A system to propagate tronchuda (Brassica oleracea var. tronchuda Bailey syn. costata L.) from main stem and side shoot cuttings was developed by removing the main stem (three to four leaves) and, later, side shoots from S-week-old plants, transplanting them into small pots, and growing them under a mist system for 4 weeks. New root growth appeared on cuttings within 3 weeks. Rooting frequency varied among cultivars and explant types. For all cultivars, side shoot cuttings rooted better than main stem cuttings (99.7% vs. 84.8%). For all cultivars, seed-propagated plants and side shoot cuttings produced leaves with significantly higher fresh weight than the main stem cuttings for three of the five cultivars. The average number of leaves per plant for four cultivars was, however, not significantly affected by propagation method. Average leaf count and fresh weight per plant were significantly higher for `Portuguesa' than for `Ana Maria'. `Couve Penca'. `Vilinda', and `Penca de Chaves' for all three propagation sources.

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mentioning

confidence: 99%

Propagation of Tronchuda (Brassica oleracea var. tronchuda Bailey) from Cuttings

Msikita¹,

Wilkinson²,

Skirvin³

1992

HortSci

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“…In fact, TuMV has been found to infect Ethiopian mustard in the field. Reports of natural infections in field crops in Zambia (Mingochi and Jensen, ) and the USA (Babu et al ., ) have been published. Unfortunately, none of these reports provided details of the strain characterization of the infecting TuMV isolates.…”

Section: Discussionmentioning

confidence: 99%

The apparent non‐host resistance of Ethiopian mustard to a radish‐infecting strain of Turnip mosaic virus is largely determined by the C‐terminal region of the P3 viral protein

Sardaru

Sinausía

López‐González

et al. 2018

Molecular Plant Pathology

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Two different isolates of Turnip mosaic virus (TuMV: UK 1 and JPN 1) belonging to different virus strains were tested on three different Brassica species, namely turnip (Brassica rapa L.), Indian mustard (Brassica juncea L.) and Ethiopian mustard (Brassica carinata A. Braun). Although all three hosts were readily infected by isolate UK 1, isolate JPN 1 was able to establish a visible systemic infection only in the first two. Ethiopian mustard plants showed no local or systemic symptoms, and no virus antigens could be detected by enzyme-linked immunosorbent assay (ELISA). Thus, this species looks like a non-host for JPN 1, an apparent situation of non-host resistance (NHR). Through an experimental approach involving chimeric viruses made by gene interchange between two infectious clones of both virus isolates, the genomic region encoding the C-terminal domain of viral protein P3 was found to bear the resistance determinant, excluding any involvement of the viral fusion proteins P3N-PIPO and P3N-ALT in the resistance. A further determinant refinement identified two adjacent positions (1099 and 1100 of the viral polyprotein) as the main determinants of resistance. Green fluorescent protein (GFP)-tagged viruses showed that the resistance of Ethiopian mustard to isolate JPN 1 is only apparent, as virus-induced fluorescence could be found in discrete areas of both inoculated and non-inoculated leaves. In comparison with other plant-virus combinations of extreme resistance, we propose that Ethiopian mustard shows an apparent NHR to TuMV JPN 1, but not complete immunity or extreme resistance.

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1999

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Reaction of Rape and Ethiopian Mustard Selections to Blackrot and Turnip Mosaic Virus (Tumv) in Zambia

Cited by 5 publications

References 0 publications

Propagation of Tronchuda (Brassica oleracea var. tronchuda Bailey) from Cuttings

Propagation of Tronchuda (Brassica oleracea var. tronchuda Bailey) from Cuttings

The apparent non‐host resistance of Ethiopian mustard to a radish‐infecting strain of Turnip mosaic virus is largely determined by the C‐terminal region of the P3 viral protein

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