Reaction of sugar beet S1 lines and cultivars to different isolates of Macrophomina phaseolina and Rhizoctonia solani AG-2-2IIIB

Mahmoudi, S B; Ghashghaie, Sara

doi:10.1007/s10681-012-0832-8

Cited by 8 publications

(5 citation statements)

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“…Phenotypic as well as genetic variation in the pathogen population, even from the same geographical region, has been documented, adding difficulties to the implementation of successful management strategies (Almeida et al 2003;Purkayastha et al 2006;Kaur et al 2012;Mahmoudi and Ghashghaie 2013;Almeida et al 2014). The use of highly aggressive isolates is recommended to optimize the results of screening assays (Mahmoudi and Ghashghaie 2013). In the current study, the use of the toothpick method allowed us the selection of the highly aggressive Brazilian isolate Me 248 to be used in further screenings.…”

Section: Discussionmentioning

confidence: 99%

Screening a variable germplasm collection of Cucumis melo L. for seedling resistance to Macrophomina phaseolina

et al. 2015

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reported to be useful for predicting shoot resistance, were performed, and plants were scored using a scale of disease severity. The average disease severity was calculated for each accession and was used to cluster the accession in five reaction classes. The screening revealed that sources of natural resistance to this fungus are limited. However, seedlings of seven accessions of different botanic groups displayed a resistant response to the stem inoculation, one cantaloup from Israel, one conomon accession from Korea, two wild agrestis and one acidulus from Africa, and two dudaim accessions from Middle East. The response of the F1 progenies varied from susceptibility to high resistance, the latter in progenies from the two agrestis wild types. These results suggest differences in the genetic basis of the resistance in the different selected sources. The resistant accessions are suggested to be screened under field conditions to confirm the level of resistance at adult plant stage and under stressful conditions.

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Section: Discussionmentioning

confidence: 99%

Screening a variable germplasm collection of Cucumis melo L. for seedling resistance to Macrophomina phaseolina

et al. 2015

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“…O conhecimento da variabilidade fitopatogênica é fundamental porque permite o estudo da estabilidade da resistência de genótipos do hospedeiro frente aos isolados do patógeno. Por outro lado, a falta de informações sobre a estrutura populacional do patógeno é um dos fatores limitantes para o sucesso de um programa, uma vez que não permite o estudo de uma possível interação entre genótipos e isolados (16).…”

Section: Resultsunclassified

Métodos de inoculação de Rhizoctonia solani e Macrophomina phaseolina em meloeiro (Cucumis melo)

et al. 2015

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RESUMOA utilização de métodos de inoculação constitui uma medida estratégica no estudo de resistência das cucurbitáceas. O objetivo deste trabalho foi avaliar dois métodos de inoculação de Rhizoctonia solani e Macrophomina phaseolina em meloeiro, visando o estudo de resistência. O experimento foi conduzido em casa de vegetação na Universidade Federal Rural do Semiárido, em Mossoró-RN, Brasil. Foram avaliados 05 acessos: A-09, A-16, A-18, A-22 e A-33 para R. solani e A-09, A-16, A-24, MR-1 e 'Olimpic' para M. phaseolina. Foram estudados os métodos areno-orgânico e palito de dente. Utilizou-se o delineamento inteiramente casualizado, com 5 repetições. Os isolados utilizados foram: Me 242, Me 243, Me 244 de R. Solani e I-248, I-249 e I-250 de M. phaseolina. Os acessos de meloeiro foram avaliados quanto à severidade da doença por uma escala de nota de 1 a 5. O método do palito de dente foi o mais eficiente em discriminar acessos de melão resistentes e suscetíveis e os isolados de R. solani e M. phaseolina quanto à virulência.

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“…As a result, greenhouse evaluations using colonizedbarley-grains are usually performed as a complement to field evaluations (Ruppel et al 1979;Behn et al 2012). Colonized-barley-grain preparation requires time and space, technical knowhow to produce on a large scale, as well as protection from other air-borne pathogens (Paulitz 2002;Paulitz and Schroeder 2005;Mahmoudi and Ghashghaie 2013;Webb and Calderon 2015).…”

Section: Introductionmentioning

confidence: 99%

“…As a result, greenhouse evaluations using colonized-barley-grains are usually performed as a complement to field evaluations (Ruppel et al 1979; Behn et al 2012). Colonized-barley-grain preparation requires time and space, technical know-how to produce on a large scale, as well as protection from other air-borne pathogens (Paulitz 2002; Paulitz and Schroeder 2005; Mahmoudi and Ghashghaie 2013; Webb and Calderon 2015). It can also be difficult to relate disease severities obtained with colonized-barley-grains inocula in greenhouse studies to those results with colonized-barley-grains inocula in evaluation of sugar beet cultivars in the fields.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Three Forms of Rhizoctonia solani Mediated Pathogenicity to Sugar Beet Cultivars in Greenhouse Studies

Haque

Parvin

2022

Preprint

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Rhizoctonia solani causes damping-off, as well as crown and root rot of sugar beet (Beta vulgaris L). This pathogen overwinters as sclerotia or melanized mycelia. Traditionally, the resistance of cultivars to R. solani is evaluated by scoring disease reactions of the crowns and roots of older seedlings, instead of evaluating at seed germination. Most studies that have evaluated cultivar resistance to R. solani have used colonized whole barley grains as artificial inocula. Colonized grains are prone to contamination with other pathogens and are often lost to rodents/birds when applied in the field. Considering those limitations, a study was undertaken (1) to develop in vitro methods to generate natural sclerotia of R. solani on a large scale, (2) to compare pathogenic potentials of R. solani sclerotia, mycelia, and colonized barley grains for optimization of damping-off assays, and (3) to evaluate the resistance of selected commercial cultivars to R. solani. Of the six-culture media, amended clarified V8 (ACV8) was the most suitable culture medium to grow and produce sclerotia on a large scale and 10 percent PDA was the least suitable. Three testing sizes of sclerotia were found to be equally effective in causing plant losses. Sclerotia inocula were comparable with mycelial discs and colonized barley grains in causing pre-emergence damping-off under aseptic in vitro conditions. Sclerotia also were equally or more effective than mycelia plug or barley grain inocula in reducing seedling emergence, inducing damping off, and increased root rot under greenhouse in vivo conditions. To conclude, sclerotia can be prepared on a feasible scale and used as natural inocula to screen response to R. solani on sugar beet.

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Reaction of sugar beet S1 lines and cultivars to different isolates of Macrophomina phaseolina and Rhizoctonia solani AG-2-2IIIB

Cited by 8 publications

References 20 publications

Screening a variable germplasm collection of Cucumis melo L. for seedling resistance to Macrophomina phaseolina

Screening a variable germplasm collection of Cucumis melo L. for seedling resistance to Macrophomina phaseolina

Métodos de inoculação de Rhizoctonia solani e Macrophomina phaseolina em meloeiro (Cucumis melo)

Evaluation of Three Forms of Rhizoctonia solani Mediated Pathogenicity to Sugar Beet Cultivars in Greenhouse Studies

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