Reactions in Droplets in Microfluidic Channels

Song, Helen; Chen, Delai L.; Ismagilov, Rustem F.

doi:10.1002/chin.200706277

Cited by 185 publications

(237 citation statements)

References 217 publications

(373 reference statements)

Supporting

Mentioning

234

Contrasting

Unclassified

Order By: Relevance

“…A more complicated, but intriguing, possibility to improve throughput would be to perform the entire assay in plugs. Several tools for manipulating plugs have been published, including mixing with streams, reagent addition, and splitting [14,30,31]. Thus, it is possible to envision a system in which a chemical library is stored as a series of plugs that are then tested and assayed by MS and by-passing the transfer from multiwell plate to tubing.…”

Section: Overall Throughputmentioning

confidence: 99%

Rapid and label-free screening of enzyme inhibitors using segmented flow electrospray ionization mass spectrometry

Pei

Kennedy

2010

J. Am. Soc. Mass Spectrom.

View full text Add to dashboard Cite

Electrospray ionization mass spectrometry (ESI-MS) is an attractive analytical tool for high-throughput screening because of its rapid scan time and ability to detect compounds without need for labels. Impediments to the use of ESI-MS for screening have been the relatively large sample consumed and slow sample introduction rates associated with commonly used flow injection analysis. We have previously shown that by segmenting nanoliter plugs of sample with air, an array of discrete samples can be delivered to a platinum-coated emitter tip for ESI-MS analysis with throughput as high as 0.8 Hz and carry-over between samples less than 0.1%. This method was applied to screening for inhibitors of acetylcholinesterase as a demonstration of the potential of segmented flow ESI-MS for such applications. Each enzyme assay consumed 10 nL of sample. At 1 L/min infusion rate, 102 samples were analyzed, corresponding to a 0.65 Hz sample analysis rate. Linear quantification of choline was achieved from 200 M to 10 mM using this method and Z= values were over 0.8 for the assay. Detailed pharmacologic dose-response curves of selected inhibitors were also measured in high-throughput fashion to validate the method. (J Am Soc

show abstract

Section: Overall Throughputmentioning

confidence: 99%

Rapid and label-free screening of enzyme inhibitors using segmented flow electrospray ionization mass spectrometry

Pei

Kennedy

2010

J. Am. Soc. Mass Spectrom.

View full text Add to dashboard Cite

show abstract

“…Droplets are natural candidates for use as microreactors, since they transport fluid with no dispersion and may be formed and manipulated using microfluidic techniques [1,2,3,4]. Indeed, a drop may be formed with a known composition and volume [5,6,7] and transported by an inert fluid without loss of the solute species and without cross-contamination [8].…”

Section: Introductionmentioning

confidence: 99%

An optical toolbox for total control of droplet microfluidics

2007

View full text Add to dashboard Cite

The use of microfluidic drops as microreactors hinges on the active control of certain fundamental operations, such as droplet formation, transport, division and fusion. Recent work has demonstrated that local heating from a focused laser can apply a thermocapillary force on a liquid interface sufficient to block the advance of a droplet in a microchannel (Baroud et al., Phys. Rev. E. V 75, p.046302). Here, we demonstrate the usefulness of this optical approach by implementing the operations mentioned above, without the need for any special microfabrication or moving parts. Building blocks such as a droplet valve, sorter, fuser, or divider are shown, as is the combination of a valve and fuser using a single laser spot.

show abstract

“…Droplet-based microfluidic systems provide many new methods to perform reactions on a small scale [1]. Applications of these methods include chemical and biochemical screening, synthesis of particles [2], dynamic behavior analysis of single cells [3], directed evolution of enzymes [4], protein crystallization, polymerase chain reaction and analysis of gene expression [5] by encapsulating cells or different reagents in droplets with their volumes ranging from the nano-to femtoliter and the droplets usually is water-in-oil droplets, in which "water" is referred as dispersed phase while "oil" is referred as continuous phase.…”

Section: Introductionmentioning

confidence: 99%

Formation and Storage of on Demand Water-in-oil Picoliter Droplets Actuated by Microfluidic Pulse Inertia Force

Wang¹,

Zhang²,

Liu³

et al. 2015

IJMMM

View full text Add to dashboard Cite

Abstract-Droplet generating in an immiscible liquid is the core technology of the droplet based biochemistry analysis. A new method, not based on micro-fluidic chip, is here proposed to produce micro scale aqueous phase droplets in oil phase liquid. The principle of the droplet generator is to actuate a hollow PZT stack by a signal generator and a voltage amplification to provide an enough pulse inertia force for a glass micro-nozzle and aqueous phase liquid inside to eject droplets. The minimum diameter of the produced spherical droplets is below 10 μm, which is below 1 pL. The range of droplets formation frequency is 2 Hz~50 Hz. A fused silica capillary is used to store the produced droplets by a designed low-pressure suction apparatus and the distance between the neighboring droplets is controlled by the low-pressure value. The proposed method in this article may be a promising approach for droplet-based single cell encapsulating and analyzing.Index Terms-Microfluids, emulsions, pulse inertia force, picoliter droplets.

show abstract

Reactions in Droplets in Microfluidic Channels

Cited by 185 publications

References 217 publications

Rapid and label-free screening of enzyme inhibitors using segmented flow electrospray ionization mass spectrometry

Rapid and label-free screening of enzyme inhibitors using segmented flow electrospray ionization mass spectrometry

An optical toolbox for total control of droplet microfluidics

Formation and Storage of on Demand Water-in-oil Picoliter Droplets Actuated by Microfluidic Pulse Inertia Force

Contact Info

Product

Resources

About