Reactions to a bovine collagen implant

Charrière, G; Bejot, Marie; Schnitzler, Liliane; Ville, G.; Hartmann, Daniel

doi:10.1016/s0190-9622(89)70330-3

Cited by 146 publications

(34 citation statements)

References 14 publications

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“…In addition, control over their mechanical properties and degradability appears to vary. On the other hand, other biodegradable synthetic polymers, such as poly L-lactic acid (PLLA), could be a good choice as the base for producing scaffolds using electrospinning techniques, because they elicit low immunogenic response [5]. It has been reported that electrospun fibers developed using collagen exhibit osteoconductivity and osteoinductivity in tissue engineering applications.…”

Section: Introductionmentioning

confidence: 99%

A Comparative Study on In Vitro Osteogenic Priming Potential of Electron Spun Scaffold PLLA/HA/Col, PLLA/HA, and PLLA/Col for Tissue Engineering Application

et al. 2014

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A comparative study on the in vitro osteogenic potential of electrospun poly-L-lactide/hydroxyapatite/collagen (PLLA/HA/Col, PLLA/HA, and PLLA/Col) scaffolds was conducted. The morphology, chemical composition, and surface roughness of the fibrous scaffolds were examined. Furthermore, cell attachment, distribution, morphology, mineralization, extracellular matrix protein localization, and gene expression of human mesenchymal stromal cells (hMSCs) differentiated on the fibrous scaffolds PLLA/Col/HA, PLLA/Col, and PLLA/HA were also analyzed. The electrospun scaffolds with a diameter of 200–950 nm demonstrated well-formed interconnected fibrous network structure, which supported the growth of hMSCs. When compared with PLLA/H%A and PLLA/Col scaffolds, PLLA/Col/HA scaffolds presented a higher density of viable cells and significant upregulation of genes associated with osteogenic lineage, which were achieved without the use of specific medium or growth factors. These results were supported by the elevated levels of calcium, osteocalcin, and mineralization (P<0.05) observed at different time points (0, 7, 14, and 21 days). Furthermore, electron microscopic observations and fibronectin localization revealed that PLLA/Col/HA scaffolds exhibited superior osteoinductivity, when compared with PLLA/Col or PLLA/HA scaffolds. These findings indicated that the fibrous structure and synergistic action of Col and nano-HA with high-molecular-weight PLLA played a vital role in inducing osteogenic differentiation of hMSCs. The data obtained in this study demonstrated that the developed fibrous PLLA/Col/HA biocomposite scaffold may be supportive for stem cell based therapies for bone repair, when compared with the other two scaffolds.

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Section: Introductionmentioning

confidence: 99%

A Comparative Study on In Vitro Osteogenic Priming Potential of Electron Spun Scaffold PLLA/HA/Col, PLLA/HA, and PLLA/Col for Tissue Engineering Application

et al. 2014

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“…Collagen molecules are stable in acidic solutions at low temperatures and are capable of forming nanofibrils that respond to body temperature and neutral pH [6, 7], permitting use of collagen as an injectable hydrogel [8, 9]. Among biocompatible polymers, collagen is one of the most preferred for gel systems because of its many advantages, including biocompatibility, bioabsorbability, and cell adhesiveness [10].…”

Section: Introductionmentioning

confidence: 99%

Temperature-Responsive Gelation of Type I Collagen Solutions Involving Fibril Formation and Genipin Crosslinking as a Potential Injectable Hydrogel

Yunoki

Ohyabu

Hatayama

2013

International Journal of Biomaterials

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We investigated the temperature-responsive gelation of collagen/genipin solutions using pepsin-solubilized collagen (PSC) and acid-solubilized collagen (ASC) as substrates. Gelation occurred in the PSC/genipin solutions at genipin concentrations 0–2 mM under moderate change in temperature from 25 to 37°C. The PSC/genipin solutions exhibited fluidity at room temperature for at least 30 min, whereas the ASC/genipin solutions rapidly reached gel points. In specific cases PSC would be preferred over ASC as an injectable gel system. The temperature-responsive gelation of PSC/genipin solutions was due to temperature responses to genipin crosslinking and collagen fibril formation. The elastic modulus of the 0.5% PSC/genipin gel system could be adjusted in a range of 2.5 to 50 kPa by the PSC and genipin concentrations, suggesting that a PSC/genipin solution is a potential injectable gel system for drug and cell carriers, with mechanical properties matching those of living tissues.

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“…The extensive use of collagen in medicine, dentistry, and pharmacology is also related to its natural properties, including hemostatic activity, biodegradability, low allergenicity with high antigenicity and biocompatibility [8]. Furthermore, it is an efficient porous matrix (scaffold) with specific physical, chemical, and biological features that are of interest for multiple bioengineering and cell culture applications [9][10][11][12][13][14][15]. Type I collagen is currently the gold standard in the field of tissue-engineering [14].…”

Section: Introductionmentioning

confidence: 99%

Extraction and characterization of highly purified collagen from bovine pericardium for potential bioengineering applications

Santos

Menezes-Silva²,

Dumont³

et al. 2013

Materials Science and Engineering: C

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Reactions to a bovine collagen implant

Cited by 146 publications

References 14 publications

A Comparative Study on In Vitro Osteogenic Priming Potential of Electron Spun Scaffold PLLA/HA/Col, PLLA/HA, and PLLA/Col for Tissue Engineering Application

A Comparative Study on In Vitro Osteogenic Priming Potential of Electron Spun Scaffold PLLA/HA/Col, PLLA/HA, and PLLA/Col for Tissue Engineering Application

Temperature-Responsive Gelation of Type I Collagen Solutions Involving Fibril Formation and Genipin Crosslinking as a Potential Injectable Hydrogel

Extraction and characterization of highly purified collagen from bovine pericardium for potential bioengineering applications

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