Reactivation in UV inactivated E. coli K12 by cell-free extracts

Korgaonkar, K. S.; Raut, Smita

doi:10.1016/0303-2647(67)90009-3

Cited by 3 publications

(2 citation statements)

References 15 publications

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“…The first investigations concerning this problem were performed in the 1960s to the early 1970s on filament strains of E coli as a target for irradiation and different strains of E coli as a source of AM substances. Partial reactivation of E coli wild-type (filament) cells was possible by the treatment with ce"-free extract or its nucleoprotein fraction obtained from nonirradiated isogenic cells (Korgaonkar and Raut, 1967). X-irradiated E coli 8, a filament-forming strain, showed an improved survival of X rays after treatment with its own extract.…”

Section: Introductionmentioning

confidence: 99%

Antimutagenic and reactivative activities of dairy propionibacteria

Vorobjeva

Khodjaev

Cherdinceva

1995

Lait

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Summary -Cells of Propionibacterium freudenreichii subsp shermanii lower the mutagenicity of 4-nitroquinoline-1-oxide in the tester strain Salmonella typhimurium TA 100 in the modified Ames test. The bacterial adaptation to the mutagen presence in the medium is demonstrated. It is possibly Iinked to induction of an antimutagenic activity. A dialysed fraction of soluble proteins of the extract (dialysate) lowers the mutagenicity of UV-C light in Salmonella typhimurium and manifests protective and reactivative effects on UV-B-and UV-C-irradiated cells of S typhimurium, Escherichia coli and the yeast Candida guilliermondii. The reactivation does not occur alter the cell irradiation with a light of total optical spectrum and visible light. (The last was studied with the yeasts.) These data serve as an indirect indication of the dialysate proteins participation in the repair of DNA injured by UV-B and UV-C irradiation. The reactivation by dialysate occurs in E coli and the yeasts Saccharomyces cerevisiae and C guilliermondii, inactivated by heating. The reactivative activity of the dialysate was localized in its 2 subfractions that manifests in E coli inactivated not only by UV light but also by heating.

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Section: Introductionmentioning

confidence: 99%

Antimutagenic and reactivative activities of dairy propionibacteria

Vorobjeva

Khodjaev

Cherdinceva

1995

Lait

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“…Korgaonkal et al isolated the active substance from cell lysate by treatment with EDTA-SDS. 23) This substance appears to be . a nucleoprotein.…”

Section: Di~cussionmentioning

confidence: 99%

Characterization of Substances that Restore Impaired Cell Division of UV-IrradiatedE. coliB

Yoshiyama

Shimoii

Tamura

1981

Agricultural and Biological Chemistry

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Effect of Incubation with Bacterial Extract on the Survival of X-irradiated Bacteria

Mukherjee¹,

Bhattacharjee²

1971

Journal of General Microbiology

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SUMMARYX-irradiated Escherichia coli B, a filament-forming strain, showed improved survival of X-rays after treatment with its own extract, either in liquid or on solid media. Extract-promoted recovery was not shown by a non-filamentforming strain E. coli B,-~. We failed to observe liquid holding recovery of X-irradiated bacteria in the absence of extract. Most experiments were performed in liquid medium where the increased number of survivors depended on the incubation period and the extract concentration. In about 30 min. the increase reached a saturation value; the maximum number of survivors was obtained using a certain (optimal) concentration of extract which was then employed in all subsequent experiments. The activity of the extract was completely destroyed by heating at 52' for 40 min. No activity was detected in the DNA, RNA or ribosomal fractions of the extract. The soluble remainder, however, was fully active, suggesting that soluble protein was the active principle.

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Reactivation in UV inactivated E. coli K12 by cell-free extracts

Cited by 3 publications

References 15 publications

Antimutagenic and reactivative activities of dairy propionibacteria

Antimutagenic and reactivative activities of dairy propionibacteria

Characterization of Substances that Restore Impaired Cell Division of UV-IrradiatedE. coliB

Effect of Incubation with Bacterial Extract on the Survival of X-irradiated Bacteria

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