Reactivation of immobilized acetyl cholinesterase in an amperometric biosensor for organophosphorus pesticide

Gulla, K.C.; Gouda, M.D.; Thakur, M. S.; Karanth, N. G.

doi:10.1016/s0167-4838(02)00268-6

Cited by 64 publications

(38 citation statements)

References 13 publications

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“…[24,25] The activity of the immobilised enzyme on the electrode was inhibited after pesticide detection. For this reason, most enzymatic biosensors can only be used once, which leads to high preparation cost, short use span and limits the actual application of the biosensor.…”

Section: Regeneration Of Achementioning

confidence: 99%

Amperometric acetylcholinesterase biosensor for pesticides monitoring utilising iron oxide nanoparticles and poly(indole-5-carboxylic acid)

Chauhan

Narang

Jain

2015

Journal of Experimental Nanoscience

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A new electrochemical sensing device was constructed for determination of pesticides. In this report, acetylcholinesterase was bioconjugated onto hybrid nanocomposite, i.e. iron oxide nanoparticles and poly(indole-5-carboxylic acid) (Fe 3 O 4 NPs/Pin5COOH) was deposited electrochemically on glassy carbon electrode. Fe 3 O 4 NPs was showed as an amplified sensing interface at lower voltage which makes the sensor more sensitive and specific. The enzyme inhibition by pesticides was detected within concentrations ranges between 0.1À60 and 1.5À70 nM for malathion and chlorpyrifos, respectively, under optimal experimental conditions (sodium phosphate buffer, pH 7.0 and 25 C). Biosensor determined the pesticides level in water samples (spiked) with satisfactory accuracy (96%À100%). Sensor showed good storage stability and retained 50% of its initial activity within 70 days at 4 C.

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Section: Regeneration Of Achementioning

confidence: 99%

Amperometric acetylcholinesterase biosensor for pesticides monitoring utilising iron oxide nanoparticles and poly(indole-5-carboxylic acid)

Chauhan

Narang

Jain

2015

Journal of Experimental Nanoscience

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“…Substrat ilavesi ile elde edilen biyosensör cevabı (I0) ve pestisit ilavesi ile elde edilen biyosensör cevabı (I1) Şekil 1'de gösterildi. Elde edilen verilerden yararlanarak deltametrinin CAT biyosensörünü hangi ölçüde inhibe ettiği hesaplandı (% I) [14,15].…”

Section: Cat Biyosensörünün çAlışma Ilkesiunclassified

Katalaz Temelli PANI Biyosensörü ile Deltametrinin Belirlenmesi

Paluzar¹,

Özcan²

2017

SDÜ Fen Bil Enst Der

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Özet: Pestisitler, tarım alanı başta olmak üzere ürünlerde istenmeyen organizmaların yok edilmesinde yüzyıllardan beri kullanılan kimyasallardır. Özellikle tarım sektöründeki kullanım artışından dolayı, pestisitler en önemli çevresel kirleticiler arasında yer almaktadır. Pestisitlerin dünyada yaygınlaşan kullanımları, bu maddelerin çevresel analizlerine gösterilen ilgiyi arttırmaktadır. Pestisit analizleri genellikle GC ve HPLC gibi yöntemler ile gerçekleştirilmektedir. Ancak bu metodlar genellikle bir takım ön işlemleri gerektirmektedir ve yerinde analizler için kullanımları uygun değildir. Enzim inhibisyonlarını temel alan biyosensör sistemleri ise bu amaca yönelik oldukça kullanışlı sistemlerdir. Bu nedenle bu çalışmada polianilin (PANI) iletken polimer kullanılarak deltametrin tayini için katalaz (CAT) inhibisyonu esaslı bir biyosensör geliştirildi. Geliştirilen biyosensörün optimizasyonu ve karakterizasyonuna ilişkin parametreler incelenerek, deltametrin varlığında lineer inhibisyon aralığı belirlendi. Deltametrin için biyosensörün standart sapma ve varyasyon katsayıları sırasıyla ± 0,0353 ve % 2,33 olarak hesaplandı. Geliştirilen biyosensörün deltametrin tayin sınırı 0,5-2,5 µM olarak belirlendi. Çalışmanın son kısmında, CAT temelli PANI biyosensörü ile atık su örneklerinde deltametrin tayini gerçekleştirilerek, elde edilen sonuçlar spektrofotometre ile elde edilen verilerle kıyaslandı. Catalase Immobilized PANI Biosensor for Detetion of DeltamethrineKeywords Deltamethrine, Pesticide, Catalase, Biosensor Abstract: Pesticides are chemicals that have been used for centuries in the destruction of unwanted organisms in products, especially in agriculture. Pesticides are among the most important environmental pollutants, especially due to the increased use in the agricultural sector. The widespread use of pesticides in the world, increases the interest for environmental analysis of these substances. Pesticide analyzes are usually carried out by methods such as GC and HPLC. However, these methods often require some pretreatments and are not suitable for on-site analysis. Biosensor systems based on enzyme inhibition are quite useful systems for this purpose. For this reason, a biosensor based on catalase (CAT) inhibition was developed for deltamethrin using polyaniline (PANI) conductive polymer. By examining the parameters for the optimization and characterization of the developed biosensor, the linear inhibition interval in the presence of deltamethrin was determined. The standard deviation and variation coefficients of the biosensor were calculated as ± 0.0353 and 2.33%, respectively, for deltamethrin. Detection limit of deltamethrin in the developed biosensor was determined as 0.5-2.5 μM. In the last part of the study, the results obtained for determination of deltamethrine in waste water samples by using CAT-PANI biosensor were compared with spectrophotometer results.

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“…Two oximes viz., 1,1-trimethylene bis 4 -formylpyridinium bromide dioxime (TMB-4) and pyridine 2-aldoxime methiodide (2-PAM) were compared for the reactivation of the immobilized AChE. TMB-4 was found to be a more efficient reactivator under repeated use retaining more than 60 % of initial activity (Gulla et al 2002). ii.…”

Section: Free Radicals and Antioxidantsmentioning

confidence: 99%

Biosensors in food processing

2012

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Optical based sensing systems that measure luminescence, fluorescence, reflectance and absorbance, etc., are some of the areas of applications of optical immunosensors. Immunological methods rely on specific binding of an antibody (monoclonal, polyclonal or engineered) to an antigen. Detection of specific microorganisms and microbial toxins requires immobilization of specific antibodies onto a given transducer that can produce signal upon attachment of typical microbe/microbial toxins. Inherent features of immunosensors such as specificity, sensitivity, speed, ease and on-site analysis can be made use for various applications. Safety of food and environment has been a major concern of food technologists and health scientists in recent years. There exists a strong need for rapid and sensitive detection of different components of foods and beverages along with the food borne and water borne pathogens, toxins and pesticide residues with high specificity. Biosensors present attractive, efficient alternative techniques by providing quick and reliable performances. There is a very good potential for application of biosensors for monitoring food quality and safety in food and bioprocessing industries in India.

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Reactivation of immobilized acetyl cholinesterase in an amperometric biosensor for organophosphorus pesticide

Cited by 64 publications

References 13 publications

Amperometric acetylcholinesterase biosensor for pesticides monitoring utilising iron oxide nanoparticles and poly(indole-5-carboxylic acid)

Amperometric acetylcholinesterase biosensor for pesticides monitoring utilising iron oxide nanoparticles and poly(indole-5-carboxylic acid)

Katalaz Temelli PANI Biyosensörü ile Deltametrinin Belirlenmesi

Biosensors in food processing

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