Reactive nitrogen species induced catalases promote a novel nitrosative stress tolerance mechanism in Vibrio cholerae

Patra, Sourav Kumar; Samaddar, Sourabh; Sinha, Nilanjan; Ghosh, Sudakshina

doi:10.1016/j.niox.2019.04.002

Cited by 13 publications

(9 citation statements)

References 40 publications

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“…Genes for those proteins are also found in some environmental bacteria, including ammonium-oxidizing bacteria (Casciotti and Ward, 2001), some Rhodocyclacles and some Burkholderiales (Figure 2 and Supplementary Figure S1). NnrS is a cuproprotein linked to nitrosative stress (Patra et al, 2019). It is present in most β proteobacteria, at several copies in Rhodocyclales, Nitrosomonadales, Neisseriales, and some Burkholderiales.…”

Section: Resultsmentioning

confidence: 99%

Relationships Between Copper-Related Proteomes and Lifestyles in β Proteobacteria

et al. 2019

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Copper is an essential transition metal whose redox properties are used for a variety of enzymatic oxido-reductions and in electron transfer chains. It is also toxic to living beings, and therefore its cellular concentration must be strictly controlled. We have performed in silico analyses of the predicted proteomes of more than one hundred species of β proteobacteria to characterize their copper-related proteomes, including cuproproteins, i.e., proteins with active-site copper ions, copper chaperones, and copper-homeostasis systems. Copper-related proteomes represent between 0 and 1.48% of the total proteomes of β proteobacteria. The numbers of cuproproteins are globally proportional to the proteome sizes in all phylogenetic groups and strongly linked to aerobic respiration. In contrast, environmental bacteria have considerably larger proportions of copper-homeostasis systems than the other groups of bacteria, irrespective of their proteome sizes. Evolution toward commensalism, obligate, host-restricted pathogenesis or symbiosis is globally reflected in the loss of copper-homeostasis systems. In endosymbionts, defense systems and copper chaperones have disappeared, whereas residual cuproenzymes are electron transfer proteins for aerobic respiration. Lifestyle is thus a major determinant of the size and composition of the copper-related proteome, and it is particularly reflected in systems involved in copper homeostasis. Analyses of the copper-related proteomes of a number of species belonging to the Burkholderia, Bordetella, and Neisseria genera indicates that commensals are in the process of shedding their copper-homeostasis systems and chaperones to greater extents yet than pathogens.

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Section: Resultsmentioning

confidence: 99%

Relationships Between Copper-Related Proteomes and Lifestyles in β Proteobacteria

et al. 2019

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“…From these facts, we propose that in a nitrate deprived anaerobic growth condition, in-vivo nitration and de-nitration is a cyclical phenomenon which is governed by controlled oxidative condition of cell and can perform as a sole source of respiration just to keep cells viable. Apart from this, in our previous studies we showed the role of S-nitrosoglutathione Reductase (GSNOR) 21 and Catalases (KatB & KatG) 22 in combating nitrosative stress mediated by GSNO and peroxynitrite respectively. Hence, in-vivo nitration might also be an advanced evolutionary adapted mechanism of nitrosative stress tolerance found in V. cholerae , as it restricts proteome to unnecessary PTN in nitroso-oxidative stress prevailing environment such as inside human gut.…”

Section: Discussionmentioning

confidence: 91%

In-vivo protein nitration and de-nitration facilitateVibrio choleraecell survival under anaerobic condition: Consequences of Nitrite induced protein nitration

Sinha²,

Chakraborty³

et al. 2021

Preprint

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Protein tyrosine nitration (PTN), a highly selective post translational modification, occurs in both prokaryotic and eukaryotic cells under nitrosative stress1. It is reported that the activities of many proteins are altered due to PTN2. PTN is found to be associated with many pathophysiological conditions like neurodegenerative and cardiac diseases etc.3. However, its physiological function is not yet clear. Like all other gut pathogens Vibrio cholerae also faces nitrosative stress in the gut environment which makes its proteome more vulnerable to PTN. Here, we report for the first time in-vivo PTN in V. cholerae. We show that in-vivo protein nitration is nitrite dependent and nitration-denitration phenomenon actually facilitates V. cholerae cell survival in anaerobic or hypoxic condition. In our study, we found that the extent of in-vivo nitration is negatively correlated with the intracellular nitrite content and maximum nitration occurs during log phase of V. cholerae. Most interestingly, a significant denitration was associated with increase in intracellular nitrate content during anaerobic incubation of aerobically grown late log phase cultures. In-vivo nitration could provide an avenue for toxic nitrite storage and nitrosative stress tolerance mechanism in many gut pathogens, whereas denitration could supply nitrate for cell survival in anaerobic nitrate deficient environment.

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“…stress response enzyme (Patra et al, 2019), was also increased in the treated cells, and this might be involved to detoxify reactive species that were generated by the action of ac.NaNO 2 (Bhattacharjee et al, 2010;Gebicka & Didik, 2009;Navarro et al, 2020;Sahoo et al, 2009). In S. cerevisiae, ADH1, ADH3, ADH4 and ADH5 produce ethanol from acetaldehyde, whereas ADH2 is involved in the reverse reaction i.e.…”

Section: Discussionmentioning

confidence: 97%

“…Reactive nitrogen species are formed during the reaction between reactive oxygen species (ROS) and nitric oxide (NO) (Peluffo & Radi, 2007;Ridnour et al, 2004). Alteration of the redox homeostasis along with the modification of macromolecules like DNA, proteins and lipids are considered to be the major consequences of nitrosative stress that leads to the modification of physicochemical properties of the cell (Carballal et al, 2014;Kurutas, 2016;Patra et al, 2017Patra et al, , 2019. Under nitrosative stress, this structural modification of proteins and enzymes by protein tyrosine nitration (PTN) and S-nitrosylation may result in the alteration of their activity.…”

Section: Introductionmentioning

confidence: 99%

Variation in glucose metabolism under acidified sodium nitrite mediated nitrosative stress in Saccharomyces cerevisiae

Sengupta

Nath

Bhuyan

et al. 2022

Journal of Applied Microbiology

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Aims:The work aimed to understand the important changes during glucose metabolism in Saccharomyces cerevisiae under acidified sodium nitrite (ac.NaNO 2 ) mediated nitrosative stress. Methods and Results:Confocal microscopy and fluorescence-activated cell sorting analysis were performed to investigate the generation of reactive nitrogen and oxygen species, and redox homeostasis under nitrosative stress was also characterized.Quantitative PCR analysis revealed that the expression of ADH genes was upregulated under such condition, whereas the ACO2 gene was downregulated. Some of the enzymes of the tricarboxylic acid cycle were partially inhibited, whereas malate metabolism and alcoholic fermentation were increased under nitrosative stress. Kinetics of ethanol production was also characterized. A network analysis was conducted to validate our findings. In the presence of ac.NaNO 2 , in vitro protein tyrosine nitration formation was checked by western blotting using pure alcohol dehydrogenase and aconitase.Conclusions: Alcoholic fermentation rate was increased under stress condition and this altered metabolism might be conjoined with the defence machinery to overcome the nitrosative stress.Significance and Impact of the Study: This is the first work of this kind where the role of metabolism under nitrosative stress has been characterized in S. cerevisiae and it will provide a base to develop an alternative method of industrial ethanol production.

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Reactive nitrogen species induced catalases promote a novel nitrosative stress tolerance mechanism in Vibrio cholerae

Cited by 13 publications

References 40 publications

Relationships Between Copper-Related Proteomes and Lifestyles in β Proteobacteria

Relationships Between Copper-Related Proteomes and Lifestyles in β Proteobacteria

In-vivo protein nitration and de-nitration facilitateVibrio choleraecell survival under anaerobic condition: Consequences of Nitrite induced protein nitration

Variation in glucose metabolism under acidified sodium nitrite mediated nitrosative stress in Saccharomyces cerevisiae

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