Reactive oxygen species and antioxidants: Relationships in green cells

Alscher, Ruth G.; Donahue, Janet L.; Cramer, Carole L.

doi:10.1034/j.1399-3054.1997.1000203.x

Cited by 307 publications

(378 citation statements)

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“…Grudarzi and Pakniyal (2009) opined that the plants defend against ROS by inducing the activities of certain antioxidative enzymes like catalase, peroxidase, glutathione reductase and superoxide dismutase. Alscher et al (2002) reported that the scavenging of ROS by increased activation of antioxidant enzymes can be attributed to efficient adaptability against abiotic stress. Apart from the enzymatic system, several epidemiological studies have suggested the importance of secondary plant metabolites, the phenolic compounds; those are consumed in reducing the incidence of ROS mediated cellular apoptosis in oxidative stress (Kinselia et al 1993;Schlesier et al 2002).…”

Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Antioxidants and ROS scavenging ability in ten Darjeeling tea clones may serve as markers for selection of potentially adapted clones against abiotic stress

Dasgupta

Biswas

Kumar

et al. 2013

Physiol Mol Biol Plants

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Ten Darjeeling tea clones (BT15/263, RR17/144, B777, T253, B157, Sundaram, HV39, AV2, K1/1 and TTV1) were collected from the experimental garden of Darjeeling Tea Research and Development Centre, Kurseong. Total phenol, flavonoids and two antioxidating enzymes (peroxidase and superoxide dismutase) were estimated. The total phenol ranged between 241 and 28 GAE mg g −1 of leaf dry weight. The highest amount obtained in four clones, B15/263 (241.47), RR17/144 (221.2), B777 (154.54) and B157 (140.23 mg g −1 ). Flavonoids were estimated as Catechin equivalent (CE) and ranged between 56.88 and 20.81 CE mg g −1 leaf dry weight. Higher amounts occurred in BT15/263 (56.88 mg g −1 ), B777 (56.69) and RR17/144 (48.63). Antioxidant activities were measured following DPPH and ABTS free radicle scavenging procedures and the results were well according to total polyphenol content among the clones (in total phenols, ranges of correlation in DPPH assay were r 2 =0.990-0.989, p≤0.05; in flavonoids r 2 =0.954, p≤0.01-0.987, p≤0.05). Similarly, ABTS percent scavenging results were quiet significant. The IC 50 values were determined for both DPPH and ABTS assay. PAGE expressions of isoforms in two antioxidative enzymes and quantification of them also varied much among the investigated clones. The incidence of total phenols, flavonoids, PRX and SOD and ROS scavenging assay in in-situ condition, might be used as biochemical markers towards the superior adaptability against abiotic stress. In the present work, four clones (B15/263, B777, RR17/144 and B157) would be designated as comparatively better suited to the predicted abiotic stress.

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Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: Antioxidants and ROS scavenging ability in ten Darjeeling tea clones may serve as markers for selection of potentially adapted clones against abiotic stress

Dasgupta

Biswas

Kumar

et al. 2013

Physiol Mol Biol Plants

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“…High Na + concentration in soils can also inhibit the absorption of potassium ions (K + ), an essential nutrient, by the roots (Wang and Han 2007). Furthermore, increased production of reactive oxygen species (ROS), known as oxidative stress, occurs as a secondary effect of high salinity (Alscher et al 1997). Because water deficits induce stomatal closure in plants, photosynthetic carbon dioxide (CO 2 ) fixation is reduced by CO 2 starvation (Ueda et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Comparative physiological analysis of salinity tolerance in rice

Ueda

Yahagi

Fujikawa

et al. 2013

Soil Science and Plant Nutrition

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Comparative physiological analysis was performed to understand the differences in the mechanisms of salinity tolerance in two rice (Oryza sativa L.) cultivars (the tolerant cultivar CFX18 and the susceptible cultivar Juma67). It was found that growth was significantly decreased in Juma67, but not in CFX18, during 10 d of salinity treatment. Under high salinity conditions, CFX18 maintained a better physiological status as determined by higher leaf water potential and a lower electrolyte leakage ratio compared to Juma67. Analysis of photosynthesis-intercellular carbon dioxide (CO 2 ) concentration response curves revealed decreases in both carboxylation efficiency and maximum photosynthetic CO 2 fixation rate in Juma67 in response to salinity stress, suggesting reduced photosynthetic capacity in this cultivar. In addition, it was observed that under high salinity conditions, Juma67 accumulated 3.5 times more sodium ions (Na + ) in the leaves compared to CFX18. To corroborate this observation, quantitative expression analysis was conducted to examine changes in the transcript levels of OsHKT1;5, which is responsible for the regulation of Na + accumulation in shoots. It was found that salinity stress repressed expression of the OsHKT1;5 gene in the leaves of Juma67, but not CFX18. These results suggest that CFX18 and Juma67 differ in their profiles of Na + accumulation under salinity stress because of differences in OsHKT1;5 expression. Thus, the mechanism of salinity tolerance seen in CFX18 relies on regulating Na + accumulation in leaves and this trait would be useful for improving salinity tolerance in rice.

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“…Hydrogen peroxide is reduced to H 2 O and destroyed by catalase (CAT) and different peroxidase such as guaiacol peroxidase (POD) and ascorbate peroxidase (APX). The decreased formation of glutathione reduces the ascorbate oxidized by APX and the glutathione is again reduced by the glutathione reductase (GR) (Alscher et al, 1997). Therefore, exogenous application of some free radical scavengers could decrease the membrane damage and increase the enzymatic defence systems against AOS.…”

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confidence: 99%