Reactive Oxygen Species and Colorectal Cancer

Sreevalsan, Sandeep; Safe, Stephen

doi:10.1007/s11888-013-0190-5

Cited by 106 publications

(99 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…To investigate whether the microbes present in the breast ducts have a metabolic activity related to malignancy or health, we examined the gene sequences associated with 18 selected KEGG metabolic pathways that have been implicated in colon cancer2021222324252627 using the PICRUSt package28. None of the 18 selected KEGG pathways were statistically significant at 5% alpha level after correcting for multiple hypothesis testing.…”

Section: Resultsmentioning

confidence: 99%

Characterization of the microbiome of nipple aspirate fluid of breast cancer survivors

Chan

Bashir

Rivas

et al. 2016

Sci Rep

179

166

View full text Add to dashboard Cite

The microbiome impacts human health and disease. Until recently, human breast tissue and milk were presumed to be sterile. Here, we investigated the presence of microbes in the nipple aspirate fluid (NAF) and their potential association with breast cancer. We compared the NAF microbiome between women with a history of breast cancer (BC) and healthy control women (HC) using 16S rRNA gene amplicon sequencing. The NAF microbiome from BC and HC showed significant differences in community composition. Two Operational Taxonomic Units (OTUs) showed differences in relative abundances between NAF collected from BC and HC. In NAF collected from BC, there was relatively higher incidence of the genus Alistipes. By contrast, an unclassified genus from the Sphingomonadaceae family was relatively more abundant in NAF from HC. These findings reflect the ductal source DNA since there were no differences between areolar skin samples collected from BC and HC. Furthermore, the microbes associated with BC share an enzymatic activity, Beta-Glucuronidase, which may promote breast cancer. This is the first report of bacterial DNA in human breast ductal fluid and the differences between NAF from HC and BC. Further investigation of the ductal microbiome and its potential role in breast cancer are warranted.

show abstract

Section: Resultsmentioning

confidence: 99%

Characterization of the microbiome of nipple aspirate fluid of breast cancer survivors

Chan

Bashir

Rivas

et al. 2016

Sci Rep

179

166

View full text Add to dashboard Cite

show abstract

“…Our results show that 3c induces apoptosis in human colorectal cancer cells by increased ROS production. ROS, which includes hydrogen peroxide, hydroxyl radical, and superoxides, are chemically active prooxidant molecules generated by incomplete reduction of oxygen [26]. Reactive oxygen species are involved in a variety of cellular physiology and pathology [27].…”

Section: Discussionmentioning

confidence: 99%

Novel derivative of aminobenzenesulfonamide (3c) induces apoptosis in colorectal cancer cells through ROS generation and inhibits cell migration

et al. 2017

View full text Add to dashboard Cite

BackgroundColorectal cancer (CRC) is the 3rd most common type of cancer worldwide. New anti-cancer agents are needed for treating late stage colorectal cancer as most of the deaths occur due to cancer metastasis. A recently developed compound, 3c has shown to have potent antitumor effect; however the mechanism underlying the antitumor effect remains unknown.Methods3c-induced inhibition of proliferation was measured in the absence and presence NAC using MTT in HT-29 and SW620 cells and xCELLigence RTCA DP instrument. 3c-induced apoptotic studies were performed using flow cytometry. 3c-induced redox alterations were measured by ROS production using fluorescence plate reader and flow cytometry and mitochondrial membrane potential by flow cytometry; NADPH and GSH levels were determined by colorimetric assays. Bcl2 family protein expression and cytochrome c release and PARP activation was done by western blotting. Caspase activation was measured by ELISA. Cell migration assay was done using the real time xCELLigence RTCA DP system in SW620 cells and wound healing assay in HT-29.ResultsMany anticancer therapeutics exert their effects by inducing reactive oxygen species (ROS). In this study, we demonstrate that 3c-induced inhibition of cell proliferation is reversed by the antioxidant, N-acetylcysteine, suggesting that 3c acts via increased production of ROS in HT-29 cells. This was confirmed by the direct measurement of ROS in 3c-treated colorectal cancer cells. Additionally, treatment with 3c resulted in decreased NADPH and glutathione levels in HT-29 cells. Further, investigation of the apoptotic pathway showed increased release of cytochrome c resulting in the activation of caspase-9, which in turn activated caspase-3 and −6. 3c also (i) increased p53 and Bax expression, (ii) decreased Bcl2 and BclxL expression and (iii) induced PARP cleavage in human colorectal cancer cells. Confirming our observations, NAC significantly inhibited induction of apoptosis, ROS production, cytochrome c release and PARP cleavage. The results further demonstrate that 3c inhibits cell migration by modulating EMT markers and inhibiting TGFβ-induced phosphorylation of Smad2 and Samd3.ConclusionsOur findings thus demonstrate that 3c disrupts redox balance in colorectal cancer cells and support the notion that this agent may be effective for the treatment of colorectal cancer.Electronic supplementary materialThe online version of this article (doi:10.1186/s12885-016-3005-7) contains supplementary material, which is available to authorized users.

show abstract

“…An elevated level of ROS in cancer cells has been shown to induce apoptosis through induction of both extrinsic as well as intrinsic pathways. Moreover, majority of natural and synthetic anticancer agents have been shown to induce apoptosis in CRC through elevation of ROS levels (Gupta et al, 2012;Sreevalsan and Safe, 2013). IR-SF significantly increased intracellular ROS levels in treated cells suggesting that it may be responsible for the activation of the death receptor pathway in HCT 116 (Zhang et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Isoledene from Mesua ferrea oleo-gum resin induces apoptosis in HCT 116 cells through ROS-mediated modulation of multiple proteins in the apoptotic pathways: A mechanistic study

et al. 2016

View full text Add to dashboard Cite

Reactive Oxygen Species and Colorectal Cancer

Cited by 106 publications

References 65 publications

Characterization of the microbiome of nipple aspirate fluid of breast cancer survivors

Characterization of the microbiome of nipple aspirate fluid of breast cancer survivors

Novel derivative of aminobenzenesulfonamide (3c) induces apoptosis in colorectal cancer cells through ROS generation and inhibits cell migration

Isoledene from Mesua ferrea oleo-gum resin induces apoptosis in HCT 116 cells through ROS-mediated modulation of multiple proteins in the apoptotic pathways: A mechanistic study

Contact Info

Product

Resources

About