Reactive Oxygen Species and Cryopreservation Promote DNA Fragmentation in Equine Spermatozoa

Baumber, Julie; Ball, Barry A.; Linfor, Jennifer J.; Meyers, Stuart A.

doi:10.1002/j.1939-4640.2003.tb02714.x

Cited by 261 publications

(148 citation statements)

References 86 publications

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“…[73][74][75] In contrast to superoxide anion, the more stable H 2 O 2 readily crosses the plasma membrane and is responsible for DNA oxidative damage. 76 Because DNA integrity is essential for achieving fertilisation and enabling embryo development in assisted reproductive technologies, the preservation of DNA during cryostorage is of extreme importance. Several studies have examined antioxidative compounds that can protect sperm DNA from cryopreservation and thawing injuries.…”

Section: Gangliosides and Spermatozoamentioning

confidence: 99%

Protective effects of exogenous gangliosides on ROS-induced changes in human spermatozoa

Gavella¹,

Lipovac²

2013

Asian J Androl

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This article summarizes the available evidence on the efficacy of gangliosides to reduce the degree of reactive oxygen species (ROS)-mediated damage. The antioxidative efficacy of exogenous gangliosides in protecting different cells encouraged us to examine their ability to protect human spermatozoa. Gangliosides are sialic acid-containing glycosphingolipids with strong amphiphilic character due to the bulky headgroup made of several sugar rings with sialic acid residues and the double-tailed hydrophobic lipid moiety. The amphiphilicity of gangliosides allows them to exist as micelles in aqueous media when they are present at a concentration above their critical micellar concentration. The protective effect of ganglioside micelles on spermatozoa is believed to stem from their ability to scavenge free radicals and prevent their damaging effects. In our study, we particularly focused our attention on the protective effect of ganglioside micelles on DNA in human spermatozoa exposed to cryopreservation. The results indicate that ganglioside micelles can modulate the hydrophobic properties of the sperm membrane to increase tolerance to DNA fragmentation, thus protecting the DNA from cryopreservation-induced damage. Further actions of ganglioside micelles, which were documented by biochemical and biophysical studies, included (i) the modulation of superoxide anion generation by increasing the diffusion barrier for membrane events responsible for signal translocation to the interior of the cell; (ii) the inhibition of iron-catalysed hydroxyl radical formation due to the iron chelation potential of gangliosides; and (iii) inhibition of hydrogen peroxide diffusion across the sperm membrane.

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Section: Gangliosides and Spermatozoamentioning

confidence: 99%

Protective effects of exogenous gangliosides on ROS-induced changes in human spermatozoa

Gavella¹,

Lipovac²

2013

Asian J Androl

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“…In addition to membrane effects, lipid peroxidation can also damage DNA. Baumber et al (2003) examined the effect of reactive oxygen species and cryopreservation on DNA of equine sperm. They demonstrated that reactive oxygen species and cryopreservation promotes DNA fragmentation in equine sperm.…”

Section: Advances In Freezing Semenmentioning

confidence: 99%

Integration of future biotechnologies into the equine industry

Squires¹

2005

Animal Reproduction Science

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There has and will continue to be reproductive techniques available that have a positive impact upon the equine breeding industry. This review focuses on semen technologies that have been developed or are in the process of being developed. The use of fluorescent dyes and flow cytometry has provided the researcher and clinician with powerful tools to evaluate several sperm attributes. These procedures have been utilized to evaluate sperm viability, acrosome status, mitochondrial status, DNA integrity and stages of capacitation. Flow cytometry allows several sperm attributes to be evaluated on thousands of spermatozoa in a matter of seconds. Development of procedures for insemination of mares with relatively small numbers of spermatozoa has the potential to change how stallions and their semen are managed. This review discusses the use of insemination of fresh, frozen and sex-sorted spermatozoa in relatively small numbers compared with conventional insemination technologies. The recent acceptance of frozen-thawed semen by many of the major breed registries has stimulated an increase in research on frozen semen. Many of the studies have focused on identifying damage during the freezing and thawing process. Numerous studies also have been conducted to modify freezing extenders so that the sperm are protected during the freezing and thawing process. The production of in vitro-produced embryos is extremely limited in the horse due to the failure of in vitro fertilization. However, intracytoplasmic sperm injection (ICSI) has been used for the production of foals from stallions that have less than typical sperm numbers or from stallions that have died and a limited quantity of frozen semen is available. This technique has been used by several laboratories to produce embryos in vitro. The breeder and veterinarian now have access to techniques that allow assessment of semen quality, improvement of procedures for freezing and thawing and insemination of mares with fewer numbers of spermatozoa. It is likely that the next decade will also produce tremendous advances in semen technologies that can be utilized in the horse industry.

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“…The most important is the production of Reactive Oxygen Species (ROS), which is excited by excessive stress, competitive sports, alcohol and drug abuse or nicotine. If produced in abundance, ROS can enter the cell nucleus, bind to the DNA and cause its fragmentation [32][33][34][35]. Several studies have reported that redox balance is deregulated in the ejaculates from infertile males, with glutathione peroxidase 4 as one of the main enzymes involved in this issue [36].…”

Section: Discussionmentioning

confidence: 99%

Sperm DNA Fragmentation is Significantly Increased in Those Men with Morphologically Abnormal Spermatozoa

Ferreyra¹

2014

JFIV Reprod Med Genet

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Purpose: To evaluate the levels of DNA fragmentation regarding to spermatozoa morphology in male infertility patients attending Andrology Laboratory at fertility center.Methods: Semen samples of 196 patients were analyzed using computer-assisted semen analysis (CASA). Sperm DNA fragmentation was measured by the sperm chromatin dispersion test. According to the values of sperm morphology, the groups were conformed by teratozoospermic (105 patients) and normozoospermic (91 patients) men.Results: Subjects' ages ranged from 21 to 68 years. Teratozoospermic men were older that the normozoospermic men (39.72 ± 7.86 vs. 36.57 ± 6.29 years; P<0.05). Values of pH were similar in both evaluated groups (P:NS). Parameters of volume, concentration, motility and vitality in teratozoospermic men were significantly lower compared to those normozoospermic patients (P<0.05). High levels of DNA fragmentation were observed in those patients with abnormal sperm morphology compared to normozoospermic men (44.31 ± 7.52 vs. 34.92 ± 5.89; P<0.05). Conclusions:Men with abnormal spermatozoa morphology showed high levels of DNA fragmentation. These significantly high percentages of sperm DNA damage will be an additional factor that drastically reduces the possibility of success in these infertile men.

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Reactive Oxygen Species and Cryopreservation Promote DNA Fragmentation in Equine Spermatozoa

Cited by 261 publications

References 86 publications

Protective effects of exogenous gangliosides on ROS-induced changes in human spermatozoa

Protective effects of exogenous gangliosides on ROS-induced changes in human spermatozoa

Integration of future biotechnologies into the equine industry

Sperm DNA Fragmentation is Significantly Increased in Those Men with Morphologically Abnormal Spermatozoa

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