Reactive oxygen species and heavy metal stress in plants: Impact on the cell wall and secondary metabolism

Berni, Roberto; Luyckx, Marie; Xuan, Xuenan; Legay, Sylvain; Sergeant, Kjell; Hausman, Jean-François; Lutts, Stanley; Cai, Giampiero; Guerriero, Gea

doi:10.1016/j.envexpbot.2018.10.017

Cited by 393 publications

(167 citation statements)

References 104 publications

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“…The JA-related processes are linked with the response to wounding, an important aspect when considering actively elongating internodes. Bast fibres indeed show a typical intrusive/ invasive mode of growth (Ageeva et al, 2005;Gorshkova et al, 2012;Guerriero et al, 2013;Lev-Yadun, 2010;Snegireva et al, 2010) which likely involves the perception of signals by neighboring parenchymatic cells (Berni, Luyckx, et al, 2018). The penetration of the middle lamellas is an event triggering wounding, but no callose deposition was observed in flax (Snegireva et al, 2010).…”

Section: Goe Analysis Of the Internodes At The Top And Middlementioning

confidence: 99%

Cell wall composition and transcriptomics in stem tissues of stinging nettle (Urtica dioica L.): Spotlight on a neglected fibre crop

et al. 2019

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Stinging nettle ( Urtica dioica L.) produces silky cellulosic fibres, as well as bioactive molecules. To improve the knowledge on nettle and enhance its opportunities of exploitation, a draft transcriptome of the “clone 13” (a fibre clone) is here presented. The transcriptome of whole internodes sampled at the top and middle of the stem is then compared with the core and cortical tissues sampled at the bottom. Young internodes show an enrichment in genes involved in the biosynthesis of phytohormones (auxins and jasmonic acid) and secondary metabolites (flavonoids). The core of internodes collected at the bottom of the stem is enriched in genes partaking in different aspects of secondary cell wall formation (cellulose, hemicellulose, lignin biosynthesis), while the cortical tissues reveal the presence of a C starvation signal probably due to the UDP‐glucose demand necessary for the thickening phase of bast fibres. Cell wall analysis indicates a difference in rhamnogalacturonan structure/composition of mature bast fibres, as evidenced by the higher levels of galactose measured, as well as the occurrence of more water‐soluble pectins in elongating internodes. The targeted quantification of phenolics shows that the middle internode and the cortical tissues at the bottom have higher contents than top internodes. Ultrastructural analyses reveal the presence of a gelatinous layer in bast fibres with a lamellar structure. The data presented will be an important resource and reference for future molecular studies on a neglected fibre crop.

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Section: Goe Analysis Of the Internodes At The Top And Middlementioning

confidence: 99%

Cell wall composition and transcriptomics in stem tissues of stinging nettle (Urtica dioica L.): Spotlight on a neglected fibre crop

et al. 2019

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“…The major impact of ROS accumulation in plants is the disruption of the integrity of cell membranes (Berni et al, ; Zhao et al, ). In our case, the electrolyte leakage was stimulated by Fe deficiency.…”

Section: Discussionmentioning

confidence: 99%

“…The major impact of ROS accumulation in plants is the disruption of the integrity of cell membranes (Berni et al, 2018;Zhao et al, 2018). In our case, the electrolyte leakage F I G U R E 5 Electrolyte leakage (a), and leave (b) and root (c) malondialdehyde content (MDA), of four genotypes of Medicago truncatula grown in the presence of Fe (C), under iron deficiency (DD) and under induced iron deficiency (ID) during the treatment period (21 days).…”

Section: Discussionmentioning

confidence: 99%

Biodiversity within Medicago truncatula genotypes toward response to iron deficiency: Investigation of main tolerance mechanisms

Kallala

M’sehli

Jelali

et al. 2019

Plant Species Biology

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Iron (Fe) deficiency is one of the major environmental stresses affecting plant production in the world. The selection of tolerant genotypes is considered an effective remediation strategy for this stress. The present study was carried out in order to investigate the biodiversity within Medicago truncatula plants in response to Fe deficiency, to identify tolerant genotypes and to assess the main tolerance mechanisms. To do this, a screening test was performed on 20 M. truncatula genotypes cultivated in minimal medium. Biometric and physiological markers were analyzed, including plant biomass, chlorophyll and root architecture. Results showed a biodiversity among the 20 genotypes. Interestingly, Fe deficiency tolerance was highest in TN8.20 and A17 genotypes. However, the lowest tolerance behavior was observed in TN1.11 and TN6.18. In order to investigate the main tolerance mechanisms, an experiment was conducted in the hydroponic system on already selected genotypes. Assessment of Fe deficiency tolerance was performed mainly on plant growth parameters, Fe (III)‐chelate‐reductase activity, rhizosphere acidification and antioxidant system defense. The relative better tolerance of A17 and TN8.20 to Fe deficiency was positively correlated with their capacity to maintain higher Fe‐acquisition efficiency in roots via rhizosphere acidification and the stimulation of Fe (III)‐chelate‐reductase activity. Moreover, tolerant genotypes showed the lowest decreases in chlorophyll content and photosynthetic activity (CO2 assimilation) compared to the sensitive ones. The efficiency of antioxidant capacity of the tolerant genotypes was revealed in stimulation of catalase (CAT) and peroxidase (POX) activities as well as accumulation of polyphenols, leading to the maintenance of cell integrity under Fe deficiency.

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“…Moreover, biomolecules such as proteins, nucleic acids, lipids and hormones are also affected by the Pb (Pourrut et al, 2011;Lanier et al, 2019). During metal stress, excess reactive oxygen species (ROS) such as superoxide anion ( • O − ), hydrogen peroxide (H 2 O 2 ), hydroxyl ion (OH • ) and malondialdehyde (MDA) are produced which cause oxidative damage to cell membranes and biomolecules (Das and Roychoudhury, 2014;Berni et al, 2019). Plants respond to excess ROS by inducing enzymatic and non-enzymatic antioxidants like superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), peroxidase (POD), glutathione reductase (GR), ascorbic acid (AsA) and reduced glutathione (GSH) that work synergistically to cope with metal toxicity (Ali et al, 2014a;Berni et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Reduced Glutathione Protects Subcellular Compartments From Pb-Induced ROS Injury in Leaves and Roots of Upland Cotton (Gossypium hirsutum L.)

et al. 2020

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Heavy metals-based changes in the plants and their alleviation through eco-friendly agents including reduced glutathione (GSH) have been widely studied. In the present experiment, we tested the alleviatory role of reduced glutathione (GSH) in seedlings of upland cotton cultivar, TM-1 under lead (Pb) toxicity. Plants were grown in the Hoagland solution containing Pb (0 µM), Pb (500 µM), GSH (50 µM), and GSH + Pb (50 µM + 500 µM). Lead exposure exacerbated hydrogen peroxide (H 2 O 2 ) and hydroxyl radical (OH • ) levels, induced lipid peroxidation (MDA), and decreased the activities of catalase (CAT) and ascorbate peroxidase (APX) in the terminal and median leaves of 28-days old cotton seedlings stressed for 10 days. However, in the primary and secondary roots, CAT activity was increased but APX decreased. Similarly, peroxidase (POD) and superoxide dismutase (SOD) activities were enhanced in the median leaves but a declining trend was observed in the terminal leaves, primary roots and secondary roots. Glutathione reductase (GR) activity, ascorbic acid (AsA) contents and GSH concentrations were increased in all parts except AsA in the median leaves. Transmission electron micrographs of Pb-treated plants exhibited deformed cell wall and cell membrane, disfigured chloroplasts and irregularly shaped mitochondria in the terminal and median leaves. Further, cell membrane, mitochondria, nucleus and other cell organelles in root cells were severely affected by the Pb. Thus their identification was little bit difficult through ultramicroscopy. External GSH stabilized leaf and root ultramorphology by stabilizing cell membranes, stimulating formation of multivesicular body vesicles, and by maintaining structural integrity of other organelles. Evidently, GSH played major alleviatory role against Pb toxicity in upland cotton.

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Reactive oxygen species and heavy metal stress in plants: Impact on the cell wall and secondary metabolism

Cited by 393 publications

References 104 publications

Cell wall composition and transcriptomics in stem tissues of stinging nettle (Urtica dioica L.): Spotlight on a neglected fibre crop

Cell wall composition and transcriptomics in stem tissues of stinging nettle (Urtica dioica L.): Spotlight on a neglected fibre crop

Biodiversity within Medicago truncatula genotypes toward response to iron deficiency: Investigation of main tolerance mechanisms

Reduced Glutathione Protects Subcellular Compartments From Pb-Induced ROS Injury in Leaves and Roots of Upland Cotton (Gossypium hirsutum L.)

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