Reactive oxygen species and nitric oxide regulate mitochondria-dependent apoptosis and autophagy in evodiamine-treated human cervix carcinoma HeLa cells

Yang, Jia; Li-jun, WU; Tashino, Shin-Ichi; Onodera, Satoshi; Ikejima, Takashi

doi:10.1080/10715760802112791

Cited by 109 publications

(87 citation statements)

References 44 publications

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“…2). These results are in line with previous reports that evodiamine-induced cell death is associated with apoptosis in different cancer cell types such as breast (8), thyroid (9), liver (10), prostate (11-14), leukemic T-lymphocyte (5,15), melanoma (6,(16)(17)(18), cervical (19,20), colon (21)(22)(23)(24), colorectal (25) and lung cancer cells (26).…”

Section: Resultssupporting

confidence: 92%

“…5B). This increase in the level of Beclin-1 gene expression is similar with the previously reported induced autophagy by evodiamine in cervical cancer carcinoma HeLa cells (20). The results indicate that Beclin-1 is involved in evodiamine-induced autophagy in SGC-7901 cells.…”

Section: Resultssupporting

confidence: 91%

“…Previously, it was reported that evodiamine induced autophagy in human cervical carcinoma HeLa cells (20). We hypothesized that evodiamine can induce the autophagy in gastric adenocarcinoma SGC-7901 cells.…”

Section: Resultsmentioning

confidence: 88%

“…Evodiamine is a naturally occurring quinolone alkaloid found in the fruit of Evodia rutaecarpa. The data of several studies concerning its cytotoxic activity in cancer cells demonstrated that evodiamine inhibited the growth of a wide variety of tumor cells, including breast (8), thyroid (9), liver (10), prostate (11)(12)(13)(14), leukemic T-lymphocyte (5,15), melanoma (6,(16)(17)(18) cervical (19,20), colon (21)(22)(23)(24), colorectal (25) and lung cells (26) through induction of apoptosis, regulation of cell cycle and reduction of invasion and metastasis.…”

Section: Introductionmentioning

confidence: 99%

“…Currently inducing autophagic cell death has been studied as a potential method for cancer therapy. Previously it was reported that evodiamine induced autophagy in human cervical carcinoma HeLa cells (20). Although, many reports elucidated the cytotoxic effects of evodiamine, yet little is known about the mechanism of evodiamine-induced cytotoxic activity in gastric cancer.…”

Section: Introductionmentioning

confidence: 99%

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Cytotoxic effect of evodiamine in SGC-7901 human gastric adenocarcinoma cells via simultaneous induction of apoptosis and autophagy

2012

Oncol Rep

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Abstract. Evodiamine, an alkaloid isolated from Evodia rutaecarpa, possesses potent anticancer activity. Although many reports have elucidated the cytotoxic effects of evodiamine in a variety of cancer cells, little is known about the mechanism of evodiamine-induced cytotoxic activity in gastric cancer cells. To date, no report has addressed the synchronized role of autophagy and apoptosis in evodiamineinduced cytotoxic activity. This study was conducted to investigate the synchronized role of autophagy and apoptosis in evodiamine-induced cytotoxic activity on SGC-7901 human gastric adenocarcinoma cells and further to elucidate the underlying molecular mechanisms. The MTT assay was used to examine the cytotoxicity of evodiamine against SGC-7901 gastric adenocarcinoma cells. The effects of evodiamine on the cell cycle and apoptosis were measured by flow cytometry and cellular morphology was observed under a phase contrast microscope. Acridine orange (AO) staining was used to detect autophagy. The expression levels of Bcl-2 and Bax were detected by Western blotting. The expression level of Beclin-1 in SGC-7901 cells was monitored by reverse transcription-polymerase chain reaction (RT-PCR). Here, we found that evodiamine significantly inhibited the proliferation of SGC-7901 cells and induced G2/M phase cell cycle arrest. Furthermore, both autophagy and apoptosis were activated during the evodiamine-induced death of SGC-7901 cells. Evodiamine-induced autophagy is partially involved in the death of SGC-7901 cells which was confirmed by using the autophagy inhibitor 3-methyladenine (3-MA). Additionally, Beclin-1 is involved in evodiamine-induced autophagy and the pro-apoptotic mechanisms of evodiamine may be associated with down-regulation of Bcl-2 and up-regulation of Bax expression. The inhibitory effects on SGC-7901 cells were associated with apoptosis, autophagy and cell cycle arrest at the G2/M phase in a dose-dependent manner. These results suggest that evodiamine is an effective natural compound for the treatment of gastric cancer and may represent a candidate for in vivo studies of monotherapies or combined antitumor therapies.

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Section: Resultssupporting

confidence: 92%

Section: Resultssupporting

confidence: 91%

Section: Resultsmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cytotoxic effect of evodiamine in SGC-7901 human gastric adenocarcinoma cells via simultaneous induction of apoptosis and autophagy

2012

Oncol Rep

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Lysosome Dysfunction Enhances Oxidative Stress‐Induced Apoptosis through Ubiquitinated Protein Accumulation in Hela Cells

Yu¹,

Huang²,

Xu³

et al. 2012

The Anatomical Record

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The role of lysosomal system in oxidative stress-induced apoptosis in cancer cells is not fully understood. Menadione is frequently used as oxidative stress model. It is indicated that menadione could induce autophagy in Hela cells. In the present study, we examined whether the lysosomal inhibitor, ammonium chloride (NH 4 Cl) could prevent the autophagy flux by inhibiting the fusion of autophagosomes with lysosomes and enhance apoptosis induced by menadione via mitochondrial pathway. The results demonstrated generation and accumulation of reactive oxygen species and increased levels of ubiquitinated proteins and GRP78 in cells treated with both menadione and NH 4 Cl. Our data indicates that lysosomal system through autophagy plays an important role in preventing menadione-induced apoptosis in Hela cells by clearing misfolded proteins, which alleviates endoplasmic reticulum stress. Anat Rec, 296:31-39,

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Rebuttal

Applegate

Stone

2010

Cathet Cardio Intervent

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Rebuttal TO THE EDITORWe read with interest the letter of Kounis et al. questioning the possible role of nickel allergy in mediating adverse events in patients with jailed side branches in the SPIRIT III study [1]. This topic has been a subject of considerable notoriety since the relationship between cutaneous allergic reactions to nickel first incited the suggestion for vascular allergic reactions to nickel-containing products contributing to stent thrombosis or restenosis. The authors are correct that the first generation paclitaxel-eluting and sirolimuseluting stents with a stainless steel stent (316L) backbone have relatively high nickel content (14% by weight) [2]. However, the author's assertion that second-generation cobalt chromium stent do not contain nickel is incorrect. L605, the cobalt chromium alloy in the everolimus-eluting Xience V/Promus drug-eluting stent has a nickel content of 10% by weight [3], and the MP35N cobalt chromium alloy of the zotarolimus-eluting endeavor drug-eluting stent has a nickel content of 35% by weight [4]. In this regard, it is worthwhile to note that animal studies evaluating the vascular responses to these two drug-eluting cobalt chromium stents have shown less inflammation than those with a 316L stainless steel backbone [5][6][7]. Because the nickel content in these two newer drug-eluting stents vary widely, the reasons(s) for their reduced inflammatory response remains to be clarified, but likely is multifactorial, due possibly to thinner stent struts, lower drug dosing, and most importantly use of less inflammatory polymers.

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Reactive oxygen species and nitric oxide regulate mitochondria-dependent apoptosis and autophagy in evodiamine-treated human cervix carcinoma HeLa cells

Cited by 109 publications

References 44 publications

Cytotoxic effect of evodiamine in SGC-7901 human gastric adenocarcinoma cells via simultaneous induction of apoptosis and autophagy

Cytotoxic effect of evodiamine in SGC-7901 human gastric adenocarcinoma cells via simultaneous induction of apoptosis and autophagy

Lysosome Dysfunction Enhances Oxidative Stress‐Induced Apoptosis through Ubiquitinated Protein Accumulation in Hela Cells

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