Reactive oxygen species as an independent marker of male factor infertility

Agarwal, Ashok; Sharma, Rakesh K.; Nallella, Kiran P.; Thomas, Anthony J.; Álvarez, Juan G.; Sikka, Suresh C.

doi:10.1016/j.fertnstert.2006.02.111

Cited by 302 publications

(222 citation statements)

References 54 publications

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“…These non-invasive infertility biomarkers would help improve management of the infertile man, which is currently based only on physical and seminal analyses, rendering more specific and individualized diagnostics and treatments [40]. This is even more important in idiopathic male infertility, which affects 25 % of infertile men, and is possibly related to functional alterations in sperm [41].…”

Section: Discussionmentioning

confidence: 99%

Unraveling the sperm proteome and post-genomic pathways associated with sperm nuclear DNA fragmentation

Intasqui

Camargo

Giudice

et al. 2013

J Assist Reprod Genet

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Purpose Sperm DNA fragmentation has been suggested as a marker for infertility diagnosis and prognosis. Hence, understanding its impact on male physiology and post-genomic pathways would be clinically important. We performed the proteomics and functional enrichment analyses of viable spermatozoa from ejaculates with low and high sperm DNA fragmentation to identify protein expression and pathways altered in association with sperm DNA fragmentation. Methods Sperm DNA fragmentation using the Comet assay and the Komet 6.0.1 software was assessed in raw samples from 89 subjects from a human reproduction service. The Low and High sperm DNA fragmentation groups were formed according to the Olive Tail Moment variable. Spermatozoa proteins from these groups were pooled and analyzed by a shotgun proteomic approach (2D nanoUPLC-ESI-MS E ). Differentially expressed proteins were used for a functional enrichment study. Results Two hundred and fifty-seven proteins were identified or quantified in sperm from the Low and High sperm DNA fragmentation groups. Of these, seventy-one proteins were exclusively or overexpressed in the Low group, whereas twenty-three proteins were exclusively or overexpressed in the High group. One hundred and sixty-three proteins were conserved between these groups. We also functionally related the differentially expressed proteins in viable spermatozoa from the groups. Processes such as triacylglycerol metabolism, energy production, protein folding, response to unfolded proteins, and cellular detoxification were found to be altered in these cells. Conclusions Sperm DNA fragmentation is associated with differential protein expression in viable spermatozoa. These proteins may potentially be used as biomarkers for sperm DNA integrity.

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Section: Discussionmentioning

confidence: 99%

Unraveling the sperm proteome and post-genomic pathways associated with sperm nuclear DNA fragmentation

Intasqui

Camargo

Giudice

et al. 2013

J Assist Reprod Genet

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“…Male germ cell membranes are sensitive to oxygen-induced damage mediated by lipid peroxidation due to their membranes are rich in polyunsaturated fatty acids [40,41]. Therefore, some antioxidants (e.g., vitamin C, vitamin E, glutathione, and coenzyme Q10) have been demonstrated to help treat male infertility [42][43][44]. The testicular cytotoxicity of DXR appears to be primarily due to oxygen-related free-radical production, and thus the administration of GTE, which reduce and/or oppose ROS, may inhibit the toxic effects of DXR.…”

Section: Discussionmentioning

confidence: 99%

Green tea extracts attenuate doxorubicin-induced spermatogenic disorders in conjunction with higher telomerase activity in mice

Sakai

Sueoka

Tanigaki

et al. 2010

J Assist Reprod Genet

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Purpose The aim of this study was to investigate the protective effect of green tea extracts against doxorubicininduced damage in the mouse testes correlating with telomerase activity. Methods Green tea extracts were administered orally. Doxorubicin was coadministered intraperitoneally. These testes were evaluated histologically and the telomerase activity was analyzed. Additional immunostaining was carried out. Results Both the sperm density and sperm motility were significantly increased in green tea extracts coadministration groups as compared to the doxorubicin-treated groups. By histological analysis, germ cell damage was greatly attenuated by green tea extracts coadministration. Telomerase activity significantly increased in association with the coadministration of green tea extracts as compared to that of doxorubicin-only groups. In all groups, human telomerase reverse transcriptase signals were mainly observed in the spermatocytes and spermatids.Conclusions These findings suggest that green tea extracts exert protective effects against doxorubicin-induced spermatogenic disorders in conjunction with higher telomerase activity levels.

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“…Furthermore, seminal oxidative stress has been reported to have a positive correlation with sperm DNA damage (19) and impairment of sperm fertilization capacity (20). Higher levels of ROS in infertile men semen compared to fertile counterparts support the in vitro data (21).…”

Section: Discussionmentioning

confidence: 52%

The Effect of Grape Seed Extract on Semen Oxidative Stress Markers in Men with Idiopathic Infertility: A Cross-Sectional Before-After Study

Soleimani

Masoumi

2017

Nephro-Urol Mon

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Background: Assisted reproductive technique is the last treatment resort for idiopathic male infertility (IMI). In recent years, antioxidants have received vast attention to treat IMI by decreasing seminal oxidative stress. Methods: In this cross-sectional before-after study, we evaluated the effects of grape seed extract (GSE) as an antioxidant on seminal chemistry. Twenty-nine patients with IMI were treated with GSE 600 mg per day for 3 months. Seminal catalase, superoxide dismutase and malondialdehyde, and serum FSH, LH, and testosterone levels were evaluated both at the beginning and at the end of the study.

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Reactive oxygen species as an independent marker of male factor infertility

Cited by 302 publications

References 54 publications

Unraveling the sperm proteome and post-genomic pathways associated with sperm nuclear DNA fragmentation

Unraveling the sperm proteome and post-genomic pathways associated with sperm nuclear DNA fragmentation

Green tea extracts attenuate doxorubicin-induced spermatogenic disorders in conjunction with higher telomerase activity in mice

The Effect of Grape Seed Extract on Semen Oxidative Stress Markers in Men with Idiopathic Infertility: A Cross-Sectional Before-After Study

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