Reactive oxygen species as glucose signaling molecules in mesangial cells cultured under high glucose

Ha, Hunjoo; Lee, Hi Bahl

doi:10.1046/j.1523-1755.2000.07704.x

Cited by 277 publications

(218 citation statements)

References 61 publications

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“…Reactive oxygen species (ROS) and oxidative stress play a crucial role in diabetic pathophysiology [41] and play important role in high glucose-induced renal injury [42,43]. The present study demonstrated enhanced redox status within the kidney tissues of type 2 diabetic rats.…”

Section: Nc Group (N=10) Dm Group (N=4) Dm+afe Group (N=8) Dm+mfe Gromentioning

confidence: 58%

Aqueous and Methanolic Extracts of Palm Date Seeds and Fruits (Phoenix dactylifera) Protects against Diabetic Nephropathy in Type II Diabetic Rats

Mousalamy¹,

Al-Shatri²

2016

Biochemistry & Physiology: Open Access

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Objective: The present study examined the effects of aqueous and methanolic extracts of palm dates (fruits and seeds) on diabetic nephropathy in a rat model of type 2 DM.Methods: Sixty male albino rats subdivided into a) normal control (NC) group, b) diabetic control (DM) group, c) aqueous fruit extract (AFE) group, d) methanolic fruit extract (MFE) group, e) aqueous seed extract (ASE) group and f) methanolic seed extract (ASE) group. Treatment with both extracts done for 15 weeks after induction of type 2 DM. by the end of experiment fasting blood glucose, serum lipid profile, creatinine, urea, uric acid, urinary albumin excretion, kidney tissue MDA, catalase, SOD, GSH were measured and kidney histopathological examination was done.Results: Diabetic untreated rats showed significant hyperglycemia and hyperlipidemia and deterioration in kidney functions and morphology with enhanced oxidative stress in kidney tissues. On other hand, rats treated with either aqueous or methanolic fruit or seed extracts showed significant improvement in all studied parameters compared to DM group (p<0.05). However, the effects of fruit extracts especially aqueous extract were more powerful than seed extracts. Conclusion:Aqueous and methanolic fruit and seed extracts of palm date protect kidneys from diabetic nephropathy in rats which might be due to their antioxidant properties.Citation: El-Mousalamy AMD, Hussein AAM, Mahmoud SA, Abdelaziz A, Shaker G (2016) Aqueous and Methanolic Extracts of Palm Date Seeds and Fruits (Phoenix dactylifera) Protects against Diabetic Nephropathy in Type II Diabetic Rats. Biochem Physiol 5: 205.

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Section: Nc Group (N=10) Dm Group (N=4) Dm+afe Group (N=8) Dm+mfe Gromentioning

confidence: 58%

Aqueous and Methanolic Extracts of Palm Date Seeds and Fruits (Phoenix dactylifera) Protects against Diabetic Nephropathy in Type II Diabetic Rats

Mousalamy¹,

Al-Shatri²

2016

Biochemistry & Physiology: Open Access

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“…Early activation of glomerular ERK1/2 in diabetes is suggested to play an important role in the progression of DN (33,35) and is consistent with a combined effect of high glucose and various growth factors, including IGF-1, PDGF-BB, and VEGF (23,54). Moreover, oxidative stress may play an important role in the progression of DN and emphasize the phosphorylation of ERK1/2 (27,28).…”

Section: Discussionmentioning

confidence: 75%

Bradykinin reduces growth factor-induced glomerular ERK1/2 phosphorylation

Cellier

Mage

Duchêne

et al. 2003

American Journal of Physiology-Renal Physiology

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Several experimental data report both mitogenic and antimitogenic effects of bradykinin (BK). To conciliate these apparent opposite effects, we hypothesized that, depending on cell context activation, BK could reduce the mitogenic effect of growth factors. Therefore, in the present study we assessed the existence of possible negative cross talk between BK and potential pathogenic growth factors in freshly isolated rat glomeruli (IG). Next, we determined whether this cross talk could be pharmacologically recruited during angiotensin-converting enzyme (ACE) inhibition in the diabetic rat. In IG from normal rats, BK, via activation of the B2 kinin receptor (B2R), causes a transient stimulation of ERK1/2 phosphorylation, whereas it inhibits ERK1/2 phosphorylation induced by IGF-1, PDGF-BB, VEGF, or basic FGF. The reduction of growth factor-induced ERK1/2 phosphorylation is abolished by an inhibitor of tyrosine phosphatase. In glomeruli from diabetic rats, hyperglycemia increased the phosphorylation level of ERK-1/2 as well as oxidative stress. The reversal of these events by ACE inhibition is mediated via B2R activation. These observations are consistent with a potential therapeutic role of BK and B2R during glomerulosclerosis.

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“…16 ROS have been shown to function as signaling molecules to activate several cellular stress-sensitive pathways that cause mesangial cell damage. 17 Besides, the oxidative stress resulting from increased ROS production can cause apoptosis in proximal tubular epithelial cells through pathways mediated by multiple caspases, such as caspase-3. 18 It has been shown that an increase in oxidative stress can induce apoptotic changes in HK2 cells, a novel human renal proximal tubular cell line, after being incubated in a high glucose concentration of 30 mmol/L for 18-48 h. 19 In contrast, decreased antioxidant activities are common events in diabetes.…”

Section: Discussionmentioning

confidence: 99%

Downregulation of uncoupling protein-2 by genipin exacerbates diabetes-induced kidney proximal tubular cells apoptosis

Chen

Tang

et al. 2014

Renal Failure

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Renal tubular epithelial cell injury is a major pathological event that contributes to the development of diabetic kidney disease (DKD). Uncoupling protein-2 (UCP2), a mitochondrial membrane protein, has been reported to participate in the regulation of reactive oxygen species (ROS) generation, which contributes to tubular cell apoptosis induced by hyperglycemia. In this study, we found that genipin, a UCP2 inhibitor, dramatically boosted oxidative stress, attenuated antioxidative capacity, and exacerbated cell apoptosis accompanied with caspase-3 activation in rat renal proximal tubular cells (NRK-52E) incubated with high glucose. The present study results suggest that manipulation of UCP2 could be important in the prevention of oxidative stress damage in renal tubular epithelial cells induced by hyperglycemia in vitro.

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Reactive oxygen species as glucose signaling molecules in mesangial cells cultured under high glucose

Cited by 277 publications

References 61 publications

Aqueous and Methanolic Extracts of Palm Date Seeds and Fruits (Phoenix dactylifera) Protects against Diabetic Nephropathy in Type II Diabetic Rats

Aqueous and Methanolic Extracts of Palm Date Seeds and Fruits (Phoenix dactylifera) Protects against Diabetic Nephropathy in Type II Diabetic Rats

Bradykinin reduces growth factor-induced glomerular ERK1/2 phosphorylation

Downregulation of uncoupling protein-2 by genipin exacerbates diabetes-induced kidney proximal tubular cells apoptosis

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