Reactive oxygen species detection-approaches in plants: Insights into genetically encoded FRET-based sensors

Anjum, Naser A.; Amreen,; Tantray, Aadil Yousuf; Khan, Nafees A.; Ahmad, Altaf

doi:10.1016/j.jbiotec.2019.12.003

Cited by 19 publications

(13 citation statements)

References 110 publications

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“…While fluorescent dyes provide beneficial spatial information for ROS levels, limitations such as dye irreversibility, working concentration, and incubation time for uptake restrict insights into the temporal dynamics of ROS regulation in guard cells. Moving forward, it will be valuable to apply new tools such as organelle localized, genetically encoded ROS sensors to fully elucidate this information (Anjum et al, 2020). Mutants defective in ROS synthesis and signaling and antioxidant synthesis highlight roles for each organelle in ROS-dependent stomatal closure.…”

Section: Discussionmentioning

confidence: 99%

The Role of ROS Homeostasis in ABA-Induced Guard Cell Signaling

2020

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The hormonal and environmental regulation of stomatal aperture is mediated by a complex signaling pathway found within the guard cells that surround stomata. Abscisic acid (ABA) induces stomatal closure in response to drought stress by binding to its guard cell localized receptor, initiating a signaling cascade that includes synthesis of reactive oxygen species (ROS). Genetic evidence in Arabidopsis indicates that ROS produced by plasma membrane respiratory burst oxidase homolog (RBOH) enzymes RBOHD and RBOHF modulate guard cell signaling and stomatal closure. However, ABAinduced ROS accumulates in many locations such as the cytoplasm, chloroplasts, nucleus, and endomembranes, some of which do not coincide with plasma membrane localized RBOHs. ABA-induced guard cell ROS accumulation has distinct spatial and temporal patterns that drive stomatal closure. Productive ROS signaling requires both rapid increases in ROS, as well as the ability of cells to prevent ROS from reaching damaging levels through synthesis of antioxidants, including flavonols. The relationship between locations of ROS accumulation and ABA signaling and the role of enzymatic and small molecule ROS scavengers in maintaining ROS homeostasis in guard cells are summarized in this review. Understanding the mechanisms of ROS production and homeostasis and the role of ROS in guard cell signaling can provide a better understanding of plant response to stress and could provide an avenue for the development of crop plants with increased stress tolerance.

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Section: Discussionmentioning

confidence: 99%

The Role of ROS Homeostasis in ABA-Induced Guard Cell Signaling

2020

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“…In vegetables, the tolerance to oxidative damage induced by stress depends largely on the rapid and efficient detoxification of ROS, a mechanism made by their defense system, comprised of enzymatic and non‐enzymatic antioxidants as the flavonoids, and polyphenols some of the most prominent agents. (Anjum, Tantray, Khan, & Ahmad, 2020). The ability to eliminate ROS in these compounds is related with the hydroxylation, methylation, glycosylation and/or acylation in their main chains (Chapman, Muhlemann, Gayomba, & Muday, 2019).…”

Section: Biological Activities From Phytochemicals In Brassicamentioning

confidence: 99%

The value of bioactive compounds of cruciferous vegetables (Brassica) as antimicrobials and antioxidants: A review

2020

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Nowadays, consumers are demanding nutrient-rich products for health optimal benefits. In this regard, Brassicaceae family plants, previously named cruciferous, group a large number of widely consumed species around the world. The popularity of Brassica is increasing due to their nutritional value and pharmacological effects. The group includes a large number of vegetable foods such as cabbages, broccoli, cauliflower, mustards as well as, oilseed rapeseed, canola, among others. In recent years, the phytochemical composition of Brassicaceae has been studied deeply because they contain many valuable metabolites, which are directly linked to different recognized biological activities. The scientific evidence confirms diverse medical properties for the treatment of chronic diseases such as obesity, type-2 diabetes, cardiovascular diseases (hypertension, stroke), cancer, and osteoporosis. The unique features of Brassicaceae family plants conferred by their phytochemicals, have extended future prospects about their use for beneficial effects on human nutrition and health worldwide. Practical applications For years, the Brassicaceae plants have been a fascinating research topic, due to their chemical composition characterized by rich in bioactive compounds. The implementation of extracts of these vegetables, causes various beneficial effects of high biological value in the treatment of diseases, owing to their bioactive properties (anti-obesity, anticancer, antimicrobial, antioxidant, hepatoprotective, cardioprotective, gastroprotective, anti-inflammatory, antianemic, and immunomodulator). Therefore, this review summarizes the chemical composition, describes the bioactive compounds isolated in the plant extracts, and highlights diverse biological activities, mainly the antimicrobial and antioxidant capacity. Brassica plants, as source of natural bioactive agents, have a great potential application to improve the human nutrition and health.

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“…Such change in Fv/Fm when plants are exposed to stressful environments is well-documented in various crops, such as maize [ 81 ], rice [ 82 ], beans [ 83 ] and tomatoes [ 84 ]. It has been concluded that the PSII reaction center may have suffered some sort of impairment by photoinactivation that is associated with oxidative damage [ 42 , 70 , 85 ]. Nevertheless, the mutant maintains an unusually high rate of photosynthesis during the onset of the water deficit stress period (Days 7 and 14) when compared with the WT, which may suggest that either structural modification protects components of the photosynthetic apparatus due to mutations, or alternatively, there is the active involvement of protective enzymes in the process.…”

Section: Discussionmentioning

confidence: 99%

EMS Derived Wheat Mutant BIG8-1 (Triticum aestivum L.)—A New Drought Tolerant Mutant Wheat Line

Roux

Burger

Vlok

et al. 2021

IJMS

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Drought response in wheat is considered a highly complex process, since it is a multigenic trait; nevertheless, breeding programs are continuously searching for new wheat varieties with characteristics for drought tolerance. In a previous study, we demonstrated the effectiveness of a mutant known as RYNO3936 that could survive 14 days without water. In this study, we reveal another mutant known as BIG8-1 that can endure severe water deficit stress (21 days without water) with superior drought response characteristics. Phenotypically, the mutant plants had broader leaves, including a densely packed fibrous root architecture that was not visible in the WT parent plants. During mild (day 7) drought stress, the mutant could maintain its relative water content, chlorophyll content, maximum quantum yield of PSII (Fv/Fm) and stomatal conductance, with no phenotypic symptoms such as wilting or senescence despite a decrease in soil moisture content. It was only during moderate (day 14) and severe (day 21) water deficit stress that a decline in those variables was evident. Furthermore, the mutant plants also displayed a unique preservation of metabolic activity, which was confirmed by assessing the accumulation of free amino acids and increase of antioxidative enzymes (peroxidases and glutathione S-transferase). Proteome reshuffling was also observed, allowing slow degradation of essential proteins such as RuBisCO during water deficit stress. The LC-MS/MS data revealed a high abundance of proteins involved in energy and photosynthesis under well-watered conditions, particularly Serpin-Z2A and Z2B, SGT1 and Calnexin-like protein. However, after 21 days of water stress, the mutants expressed ABC transporter permeases and xylanase inhibitor protein, which are involved in the transport of amino acids and protecting cells, respectively. This study characterizes a new mutant BIG8-1 with drought-tolerant characteristics suited for breeding programs.

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Reactive oxygen species detection-approaches in plants: Insights into genetically encoded FRET-based sensors

Cited by 19 publications

References 110 publications

The Role of ROS Homeostasis in ABA-Induced Guard Cell Signaling

The Role of ROS Homeostasis in ABA-Induced Guard Cell Signaling

The value of bioactive compounds of cruciferous vegetables (Brassica) as antimicrobials and antioxidants: A review

EMS Derived Wheat Mutant BIG8-1 (Triticum aestivum L.)—A New Drought Tolerant Mutant Wheat Line

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