Reactive oxygen species inhibit hyposmotic stress-dependent volume regulation in cultured rat cardiomyocytes

Díaz-Elizondo, Jessica; Chiong, Mario; Rojas‐Rivera, Diego; Olea‐Azar, Claudio; Kwon, Hyug Moo; Lavandero, Sergio

doi:10.1016/j.bbrc.2006.10.004

Cited by 18 publications

(32 citation statements)

References 27 publications

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“…It should be noted that we cannot exclude that the remaining p47 phox expression following p47 phox silencing could be sufficient to maintain the regulation of the oxidase activity. Rac1 has been shown to facilitate the stabilization of the p67 phox at the membrane bound catalytic subunits of the NADPH oxidase complex (7,12,16). However, we found that overexpression of Rac1 had no effect on swelling-induced ROS production nor the concomitant taurine release.…”

Section: Regulation Of the Nadph Oxidase In Nih3t3 Fibroblastscontrasting

confidence: 53%

“…implicated in ligand/receptor signaling, stress responses, apoptosis, proliferation, and cell volume regulation (9,16,37,59,63).…”

Section: Discussionmentioning

confidence: 99%

“…It is suggested that a NOX4 isotype plus p22 phox account for the swelling-induced increase in the ROS production in NIH3T3 cells and that the oxidase activity is potentiated by PKC and LPA but not by Ca 2ϩ . organic osmolytes; NOX4; lysophospholipids; arachidonic acid mobilization; adenosine triphosphate; calcium REACTIVE OXYGEN SPECIES (ROS), e.g., superoxides, hydroxyl radicals, and H 2 O 2 , are produced in a variety of cells upon ligand stimulation, during stress, cell proliferation, and apoptosis (9,16,59,62,63). Within recent years, it has been shown that ROS are produced also upon osmotic stress in various cell types (35,51,71) and that ROS are involved in the swellinginduced activation and inactivation of the volume-sensitive release pathway for the organic osmolyte taurine in, e.g., NIH3T3 fibroblasts (35, 38) and in the activity of the volumesensitive, outwardly rectifying Cl Ϫ channel in liver cells (71).…”

mentioning

confidence: 99%

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Volume-sensitive NADPH oxidase activity and taurine efflux in NIH3T3 mouse fibroblasts

Friis

Vorum²,

Lambert

2008

American Journal of Physiology-Cell Physiology

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Friis MB, Vorum KG, Lambert IH. Volume-sensitive NADPH oxidase activity and taurine efflux in NIH3T3 mouse fibroblasts. Am J Physiol Cell Physiol 294: C1552-C1565, 2008. First published April 16, 2008 doi:10.1152/ajpcell.00571.2007.-Reactive oxygen species (ROS) are produced in NIH3T3 fibroblasts during hypotonic stress, and H2O2 potentiates the concomitant release of the organic osmolyte taurine (Lambert IH. J Membr Biol 192: 19 -32, 2003). The increase in ROS production [5-(and-6)-carboxy-2Ј, 7Ј-dichlorodihydrofluorescein diacetate fluorescence] is detectable after a reduction in the extracellular osmolarity from 335 mosM (isotonic) to 300 mosM and reaches a maximal value after a reduction to 260 mosM. The swelling-induced ROS production is reduced by the flavoprotein inhibitor diphenylene iodonium chloride (25 M) but is unaffected by the nitric oxide synthase inhibitor N -nitro-L-arginine methyl ester, indicating that the volume-sensitive ROS production is NADPH oxidase dependent. NIH3T3 cells express the NADPH oxidase components: p22 phox , a NOX4 isotype; p47 phox ; and p67 phox (real-time PCR). Exposure to the Ca 2ϩ -mobilizing agonist ATP (10 M) potentiates the release of taurine but has no effect on ROS production under hypotonic conditions. On the other hand, addition of the protein kinase C (PKC) activator phorbol 12-myristate 13-acetate (PMA, 100 nM) or the lipid messenger lysophosphatidic acid (LPA, 10 nM) potentiates the swelling-induced taurine release as well as the ROS production. Overexpression of Rac1 or p47 phox or p47 phox knockdown [small interfering (si)RNA] had no effect on the swelling-induced ROS production or taurine release. NOX4 knockdown (siRNA) impairs the increase in the ROS production and the concomitant taurine release following osmotic exposure. It is suggested that a NOX4 isotype plus p22 phox account for the swelling-induced increase in the ROS production in NIH3T3 cells and that the oxidase activity is potentiated by PKC and LPA but not by Ca 2ϩ . organic osmolytes; NOX4; lysophospholipids; arachidonic acid mobilization; adenosine triphosphate; calcium REACTIVE OXYGEN SPECIES (ROS), e.g., superoxides, hydroxyl radicals, and H 2 O 2 , are produced in a variety of cells upon ligand stimulation, during stress, cell proliferation, and apoptosis (9,16,59,62,63). Within recent years, it has been shown that ROS are produced also upon osmotic stress in various cell types (35,51,71) and that ROS are involved in the swellinginduced activation and inactivation of the volume-sensitive release pathway for the organic osmolyte taurine in, e.g., NIH3T3 fibroblasts (35, 38) and in the activity of the volumesensitive, outwardly rectifying Cl Ϫ channel in liver cells (71). Activation of the volume-sensitive taurine release pathway in NIH3T3 fibroblasts involves specific phospholipase A 2 (PLA 2 ) (40,41), and it appears that the swelling-induced ROS production involves a NADPH oxidase, which is activated at a step downstream to the PLA 2 activation (35). Colston and coworkers (13) similarly ...

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Section: Regulation Of the Nadph Oxidase In Nih3t3 Fibroblastscontrasting

confidence: 53%

“…implicated in ligand/receptor signaling, stress responses, apoptosis, proliferation, and cell volume regulation (9,16,37,59,63).…”

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Volume-sensitive NADPH oxidase activity and taurine efflux in NIH3T3 mouse fibroblasts

Friis

Vorum²,

Lambert

2008

American Journal of Physiology-Cell Physiology

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“…7) (18). In other cell types, hypotonic shock leads to ROS production (37)(38)(39). In T. brucei brucei, however, osmotic regulation may be disrupted by peroxides produced at endogenous levels or levels of peroxides below the limits of detection in our assays.…”

Section: Discussionmentioning

confidence: 95%

“…Similarly, in rat neonatal cardiomyocytes, endogenously produced reactive oxygen species inactivate RVD during hypotonic conditions (38). In rabbit hearts, the proposed signaling cascade linking osmotic swelling and ROS production involves peroxides activating swelling-activated chloride channels (51).…”

Section: Discussionmentioning

confidence: 99%

Trypanosome Lytic Factor-1 Initiates Oxidation-stimulated Osmotic Lysis of Trypanosoma brucei brucei

Greene

Hajduk

2016

Journal of Biological Chemistry

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Human innate immunity against the veterinary pathogenTrypanosoma brucei brucei is conferred by trypanosome lytic factors (TLFs), against which human-infective T. brucei gambiense and T. brucei rhodesiense have evolved resistance. TLF-1 is a subclass of high density lipoprotein particles defined by two primate-specific apolipoproteins: the ion channel-forming toxin ApoL1 (apolipoprotein L1) and the hemoglobin (Hb) scavenger Hpr (haptoglobin-related protein). The role of oxidative stress in the TLF-1 lytic mechanism has been controversial. Here we show that oxidative processes are involved in TLF-1 killing of T. brucei brucei. The lipophilic antioxidant N,N-diphenyl-p-phenylenediamine protected TLF-1-treated T. brucei brucei from lysis. Conversely, lysis of TLF-1-treated T. brucei brucei was increased by the addition of peroxides or thiol-conjugating agents. Previously, the Hpr-Hb complex was postulated to be a source of free radicals during TLF-1 lysis. However, we found that the iron-containing heme of the Hpr-Hb complex was not involved in TLF-1 lysis. Furthermore, neither high concentrations of transferrin nor knock-out of cytosolic lipid peroxidases prevented TLF-1 lysis. Instead, purified ApoL1 was sufficient to induce lysis, and ApoL1 lysis was inhibited by the antioxidant DPPD. Swelling of TLF-1-treated T. brucei brucei was reminiscent of swelling under hypotonic stress. Moreover, TLF-1-treated T. brucei brucei became rapidly susceptible to hypotonic lysis. T. brucei brucei cells exposed to peroxides or thiol-binding agents were also sensitized to hypotonic lysis in the absence of TLF-1. We postulate that ApoL1 initiates osmotic stress at the plasma membrane, which sensitizes T. brucei brucei to oxidation-stimulated osmotic lysis.

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Downregulation of the Taurine Transporter TauT During Hypo-Osmotic Stress in NIH3T3 Mouse Fibroblasts

et al. 2012

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The present work was initiated to investigate regulation of the taurine transporter TauT by reactive oxygen species (ROS) and the tonicity-responsive enhancer binding protein (TonEBP) in NIH3T3 mouse fibroblasts during acute and long-term (4 h) exposure to low-sodium/hypo-osmotic stress. Taurine influx is reduced following reduction in osmolarity, keeping the extracellular Na+ concentration constant. TonEBP activity is unaltered, whereas TauT transcription as well as TauT activity are significantly reduced under hypo-osmotic conditions. In contrast, TonEBP activity and TauT transcription are significantly increased following hyperosmotic exposure. Swelling-induced ROS production in NIH3T3 fibroblasts is generated by NOX4 and by increasing total ROS, by either exogenous application of H2O2 or overexpressing NOX4, we demonstrate that TonEBP activity and taurine influx are regulated negatively by ROS under hypo-osmotic, low-sodium conditions, whereas the TauT mRNA level is unaffected. Acute exposure to ROS reduces taurine uptake as a result of modulated TauT transport kinetics. Thus, swelling-induced ROS production could account for the reduced taurine uptake under low-sodium/hypo-osmotic conditions by direct modulation of TauT.Electronic supplementary materialThe online version of this article (doi:10.1007/s00232-012-9416-8) contains supplementary material, which is available to authorized users.

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Reactive oxygen species inhibit hyposmotic stress-dependent volume regulation in cultured rat cardiomyocytes

Cited by 18 publications

References 27 publications

Volume-sensitive NADPH oxidase activity and taurine efflux in NIH3T3 mouse fibroblasts

Volume-sensitive NADPH oxidase activity and taurine efflux in NIH3T3 mouse fibroblasts

Trypanosome Lytic Factor-1 Initiates Oxidation-stimulated Osmotic Lysis of Trypanosoma brucei brucei

Downregulation of the Taurine Transporter TauT During Hypo-Osmotic Stress in NIH3T3 Mouse Fibroblasts

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