Reactive oxygen species production, induced by atmospheric modification, alter conidial quality of<i>Beauveria bassiana</i>

Pérez‐Guzmán, D.; Montesinos-Matías, Roberto; Arce-Cervantes, Oscar; Gómez-Quiroz, Luís Enrique; Loera, Octavio; Garza-López, Paul Misael

doi:10.1111/jam.13156

Cited by 9 publications

(6 citation statements)

References 34 publications

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“…This differentiation process is preceded by oxidant states in which reactive oxygen species (ROS) accumulate, which lead to the appearance of protein oxidation (Toledo and Hansberg ). In B. bassiana Bb 882·5, a 26% × 2 treatment led to oxidant states in which lipoperoxidation occurred (Garza‐López et al ) and ROS accumulated (Pérez‐Guzmán et al ). However, a headspace volume:gds ratio of 15 ml per gds was used for those experiments, which is more likely to represent stress by hypoxia‐anoxia.…”

Section: Resultsmentioning

confidence: 99%

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Improved conidiation from entomopathogenic fungi through 26% oxygen pulses in solid‐state culture depends on a balance between headspace volume and substrate amounts

Muñiz-Paredes

Sánchez-García

Garza-López

et al. 2019

Lett Appl Microbiol

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Oxygen‐enriched atmospheres applied as periodic pulses increased conidia production from entomopathogenic fungi in agar surface cultures. However, this advantage has not been obtained in solid‐state cultures (SSC), probably as a result of different biomass production between both culture systems. In this work, the biomass formation from two Isaria strains was limited in SSC using 5, 2·5 and 1 initial grams of substrate (gds). In the system with 5 gds, conidia production decreased in 26% oxygen‐enriched pulses compared to the normal atmosphere. Conversely, 26% oxygen pulses increased conidiation up to one order magnitude in systems with 2·5 and 1 gds, respective to the normal atmosphere. These results were explained by oxygen depletion and high CO2 accumulation in the 5 gds system. Whereas in systems with 2·5 or 1 gds, oxygen levels remained high enough to stimulate conidiation. These results were attributed to the headspace volume:gds ratio, which is suggested to be ≥48 ml per gds. This ratio is proposed as a scaling‐up criterion for bioreactor design when oxygen‐enriched pulses are used in SSC for improvement of conidia production. Significance and Impact of the Study Oxygen‐enriched atmospheres applied as periodic pulses increase conidiation in entomopathogenic fungi (EF). However, this remained restricted to agar surface cultures, since conidiation decreased when carried out in solid‐state culture (SSC) which is used as large‐scale production system. We identified that in SSC the ratio between the headspace volume containing 26% oxygen‐enriched pulses and the grams of substrate determines the conidiation response to oxygen‐enriched pulses. For the first time, oxygen‐enriched pulses increased conidiation in SSC respective to the normal atmosphere in four EF. This ratio is proposed as a bioreactor criterion design for large‐scale conidia production of EF using oxygen‐enriched pulses.

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Section: Resultsmentioning

confidence: 99%

“…Hyphal differentiation leading to conidiation is a critical factor for entomopathogenic fungi whose conidia are the infective units for the control of insect plagues (Pérez‐Guzmán et al ). Many variables affect the conidiation process, including the oxygen level in the atmosphere.…”

Section: Introductionmentioning

confidence: 99%

Improved conidiation from entomopathogenic fungi through 26% oxygen pulses in solid‐state culture depends on a balance between headspace volume and substrate amounts

Muñiz-Paredes

Sánchez-García

Garza-López

et al. 2019

Lett Appl Microbiol

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“…Second, according to a previous report in C. albicans [2], NDT80 deletion mutants also showed sensitivity to heat stress in B. bassiana. Heat stress could promote cellular ROS generation, thereby damaging certain biomolecules, such as DNA, lipids or proteins [33,36]. Antioxidant enzymes can scavenge diverse intracellular ROS [27,37].…”

Section: Plos Onementioning

confidence: 99%

A p53-like transcription factor, BbTFO1, contributes to virulence and oxidative and thermal stress tolerances in the insect pathogenic fungus, Beauveria bassiana

et al. 2021

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The p53-like transcription factor (TF) NDT80 plays a vital role in the regulation of pathogenic mechanisms and meiosis in certain fungi. However, the effects of NDT80 on entomopathogenic fungi are still unknown. In this paper, the NDT80 orthologue BbTFO1 was examined in Beauveria bassiana, a filamentous entomopathogenic fungus, to explore the role of an NDT80-like protein for fungal pest control potential. Disruption of BbTFO1 resulted in impaired resistance to oxidative stress (OS) in a growth assay under OS and a 50% minimum inhibitory concentration experiment. Intriguingly, the oxidation resistance changes were accompanied by transcriptional repression of the two key antioxidant enzyme genes cat2 and cat5. ΔBbTFO1 also displayed defective conidial germination, virulence and heat resistance. The specific supplementation of BbTFO1 reversed these phenotypic changes. As revealed by this work, BbTFO1 can affect the transcription of catalase genes and play vital roles in the maintenance of phenotypes associated with the biological control ability of B. bassiana.

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“…To apply Trichoderma as biopesticide on the field crops is necessary to do it in form of spores because they are the most infective units. It is demonstrated that spores produced by solid-state fermentation (SSF) are more resistant to the real conditions found in nature [9]. SSF is a microbial process carried out on solid materials surface on the absence of free water.…”

Section: Introductionmentioning

confidence: 99%

Production of a Biological Control Agent: Effect of a Drying Process of Solid-State Fermentation on Viability of Trichoderma Spores

Aguilar

2018

Int. J. Green Technol.

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Biological control is an environmentally effective technology for the reduction of pests through the use of natural enemies called biological control agents (BCA). Among BCA, Trichoderma is an important biopesticide fungus against several plant pathogens. The present study, shown the evaluation of a solid-state fermentation process in order to produce spores by Trichoderma harzianum. Sugarcane bagasse, wheat bran, and potato flour were evaluated as a mix of substrates to produce fungal spores. CO 2 released during mycelial growth and sporulation was monitoring by respirometry. Effect of dry air was evaluated on the decrease stage of fungal metabolism as an inducer of sporulation, and also the effect of drying on the viability of the spores produced. Under these conditions, the maximum concentration of spores was 8.4x10 9 spores/g carbon source (CS) at 96 h. Results demonstrated that dry air applied on SSF did not have influence on the increase of spore produced, but it can accelerate this process (at least 24 hours) and also to increase the spore viability (1.3 x10 9 spore/ g carbon source, with 24.7 % of viability, respectively). SSF without application of dry air showed a production of 8.9x109 spore/g CS. In parallel, the endoglucanase and exoglucanase activities were evaluated obtaining 11.6 and 0.86 U/g CS, respectively.

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Reactive oxygen species production, induced by atmospheric modification, alter conidial quality ofBeauveria bassiana

Abstract: This is the first study in which conidial infectivity and ROS production in B. bassiana has been related, enhancing the knowledge of the effect of O2 pulses in B. bassiana.

Cited by 9 publications

References 34 publications

Improved conidiation from entomopathogenic fungi through 26% oxygen pulses in solid‐state culture depends on a balance between headspace volume and substrate amounts

Improved conidiation from entomopathogenic fungi through 26% oxygen pulses in solid‐state culture depends on a balance between headspace volume and substrate amounts

A p53-like transcription factor, BbTFO1, contributes to virulence and oxidative and thermal stress tolerances in the insect pathogenic fungus, Beauveria bassiana

Production of a Biological Control Agent: Effect of a Drying Process of Solid-State Fermentation on Viability of Trichoderma Spores

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