Reactive oxygen species: rapid fire in inflammation

Ingram, Sonia; Diotallevi, Marina

doi:10.1042/bio03904030

Cited by 8 publications

(12 citation statements)

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“…34,35 Likewise, ROS exert diverse pro-inflammatory effects. 36 Furthermore, our present findings indicate that both adipocytokines affect mRNA expression of various cytokines/chemokines. In particular, these factors up-regulate the mRNA level of IL-1b, TNF, and CCL3, which all, in different ways, promote the development of inflammation.…”

Section: Discussionsupporting

confidence: 58%

“…Similarly, cysLTs and chemokine CCL2 are factors that noticeably contribute to supporting and maintaining inflammatory responses, as they stimulate various cells to the production of pro‐inflammatory mediators, augment vascular permeability, enhance cell adhesion to the vascular epithelium, and may recruit immune cells into inflamed tissues . Likewise, ROS exert diverse pro‐inflammatory effects …”

Section: Discussionmentioning

confidence: 99%

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Adipocytokines leptin and adiponectin function as mast cell activity modulators

et al. 2019

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Summary A growing body of data indicates that adipocytokines, including leptin and adiponectin, are critical components not only of metabolic regulation but also of the immune system, mainly by influencing the activity of cells participating in immunological and inflammatory processes. As mast cells (MCs) are the key players in the course of those mechanisms, this study aimed to evaluate the impact of leptin and adiponectin on some aspects of MC activity. We documented that in vivo differentiated mature tissue MCs from the rat peritoneal cavity express a receptor for leptin (OB‐R), as well as receptors for adiponectin (AdipoR1 and AdipoR2). We established that leptin, but not adiponectin, stimulates MCs to release of histamine as well as to generation of cysteinyl leukotrienes (cysLTs) and chemokine CCL2. We also found that both adipocytokines affect mRNA expression of various cytokines/chemokines. Leptin and adiponectin also activate MCs to produce reactive oxygen species. Moreover, we documented that leptin significantly augments the surface expression of receptors for cysLTs, i.e. CYSLTR1, CYSLTR2, and GPR17 on MCs, while adiponectin increases only GPR17 expression, and decreases CYSLTR2. Finally, we showed that both adipocytokines serve as potent chemoattractants for MCs. In intracellular signaling in MCs activated by leptin Janus‐activated kinase 2, phospholipase C, phosphatidylinositol 3‐kinase (PI3K), extracellular signal‐regulated kinase (ERK1/2), and p38 molecules play a part whereas the adiponectin‐induced activity of MCs is mediated through PI3K, p38, and ERK1/2 pathways. Our observations that leptin and adiponectin regulate MC activity might indicate that adipocytokines modulate the different processes in which MCs are involved.

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Section: Discussionsupporting

confidence: 58%

Section: Discussionmentioning

confidence: 99%

Adipocytokines leptin and adiponectin function as mast cell activity modulators

et al. 2019

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“…In this particular study, the stimuli for the overexpression of this protein was the high ROS levels in semen of ROS+ men. In fact, there is a direct link between high ROS levels and inflammation [36]. A previous proteomic study also identified the overexpression of S100A9 in the seminal plasma of smoking men [37], which also reflects an environment with high ROS levels.…”

Section: Discussionmentioning

confidence: 99%

Proteomic Signatures Reveal Differences in Stress Response, Antioxidant Defense and Proteasomal Activity in Fertile Men with High Seminal ROS Levels

Dias

Samanta

Agarwal

et al. 2019

IJMS

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Elevated levels of reactive oxygen species (ROS) are a major cause of male infertility. However, some men with high seminal ROS levels are still fertile. The main objective of this study was to understand the molecular mechanism(s) responsible for the preservation of fertility in those men. Semen samples from fertile men were divided into two groups: control (n = 10, ROS < 102.2 RLU/s/106 sperm) and ROS+ (n = 10, ROS > 102.2 RLU/s/106 sperm). Proteomic analysis of seminal plasma and spermatozoa was used to identify the differentially expressed proteins (DEPs) between the experimental groups, from which some proteins were validated by Western blot (WB). A total of 44 and 371 DEPs were identified between the study groups in the seminal plasma and spermatozoa, respectively. The identified DEPs were primarily involved in oxidoreductase, endopeptidase inhibitor, and antioxidant activities. We validated by WB the underexpression of NADH:ubiquinone oxidoreductase core subunit S1 (p = 0.01), as well as the overexpression of superoxide dismutase 1 (p = 0.03) and peroxiredoxin 4 (p = 0.04) in spermatozoa of ROS+ group. Our data suggest that fertile men with high ROS levels possess an effective antioxidant defense system that protects sperm proteins, as well as an active proteasomal system for degradation of defective proteins.

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“…The outcomes of these changes can be useful for cell normal live or can lead to dysfunctions. This phenomenon is at the basis of almost all human diseases including communicable infectious diseases and non-communicable diseases such as Alzheimer, Parkinson, diabetes, autoimmune diseases and inflammatory diseases [2].…”

Section: Introduction mentioning

confidence: 99%

In Vitro Antioxidant and Anti-inflammatory Potential of the Optimized Combinations of Essential Oils from Three Cameroon Grew Ocimum L.

Fokou¹,

Pierre²,

Gisele³

et al. 2020

JPP

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In the best of our knowledge, there is no report on the antioxidant and anti-inflammatory effect of the combination of essential oils from Ocimum genus. In our previous report, the combinations of Ocimum basilicum/Ocimum gratissimum, Ocimum basilicum/Ocimum urticaefolium and Ocimum gratissimum/Ocimum urticaefolium were optimized for their antifungal effect. The present report presents the spectral profile and antioxidant and anti-inflammatory effects of those optimized combinations. The optimized combinations were prepared following our previously described approach and analyzed by Fourier Transformed infrared spectroscopy for fingerprinting of essential oils involved in the combination. The radical scavenging activity was evaluated on diphenyl-picryl-hydrazyle (DPPH) and ferric reducing antioxidant power (FRAP). The antioxidant activity was determined using the protective model of Linseed oil accelerated oxidation. The anti-inflammatory activity was assayed using cyclooxygenase (COX) 1 and 2. The results indicated that the fingerprints of the combination are different from those in the essential oil analyzed alone. This denotes the physical expression of different hydrogen bonds in the mixture. Overall, the optimized combinations exerted a maximum reduction (OD = 4) effect on the ferric ion. As well, they scavenged the DPPH efficiently (90% inhibition). These combinations also successfully inhibited the auto-oxidation of flaxseed oil by reduction of cis double bonds, aldehyde and hydroxyl. The optimized combination of Ocimum basilicum and Ocimum gratissimum showed a lower inhibitory effect in comparison to the control consisting of enzyme without inhibitor. Of note, Ocimum gratissimum alone exerted an inhibitory effect on both isoform of cyclooxygenase with IC 50 = 0.23 µL/mL and 0.27 µL/mL for cyclooxygenase 1 and 2 respectively. Also, the essential oil of Ocimum basilicum exhibited a better effect on cyclooxygenase 2 (IC 50 = 0.22 µL/mL) but showed less action on cyclooxygenase 1 (IC 50 = 0.55 µL/mL). The essential oil of Ocimum urticaefolium presented the best effect on cyclooxygenase 1 (IC 50 = 0.06 µL/mL) but appeared to be nonactive on cyclooxygenase 2. Globally, the optimized combinations inhibited efficiently all the isoforms of cyclooxygenase with percentage inhibition of ≥ 67% for cyclooxygenase 2 and ≥ 98% for cyclooxygenase 1.

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Reactive oxygen species: rapid fire in inflammation

Cited by 8 publications

References 0 publications

Adipocytokines leptin and adiponectin function as mast cell activity modulators

Adipocytokines leptin and adiponectin function as mast cell activity modulators

Proteomic Signatures Reveal Differences in Stress Response, Antioxidant Defense and Proteasomal Activity in Fertile Men with High Seminal ROS Levels

In Vitro Antioxidant and Anti-inflammatory Potential of the Optimized Combinations of Essential Oils from Three Cameroon Grew Ocimum L.

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