Reactive oxygen species (ROS) activity of fine particulate matter health impacts in Addis Ababa, Ethiopia

Sricharoenvech, Piyaporn; Lai, Alexandra; Tefera, Worku; Kumie, Abera; Berhane, Kiros; Gilliland, Frank D.; Samet, Jonathan M.; Patz, Jonathan A.; Schauer, James J.

doi:10.1016/j.apr.2021.101149

Cited by 3 publications

(2 citation statements)

References 33 publications

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“…ROS can participate in the oxygen metabolism in organisms and has positive physiological effects within a certain limit . Excessive levels of ROS, including superoxide free radicals (O 2– ) and hydrogen peroxide (H 2 O 2 ) can cause peroxidation of unsaturated fatty acids and damage the structure of cell membranes.…”

Section: Resultsmentioning

confidence: 99%

“…ROS can participate in the oxygen metabolism in organisms and has positive physiological effects within a certain limit. 41 Excessive levels of ROS, including superoxide free radicals (O 2− ) and hydrogen peroxide (H 2 O 2 ) can cause peroxidation of unsaturated fatty acids and damage the structure of cell membranes. The staining results showed weak ROS fluorescence signals in the blank control (Figure 5a), whereas strong fluorescent signals were present in the treated group (Figure 5b).…”

Section: N T N Inhibition Rate (%) (mentioning

confidence: 99%

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Design and Synthesis of Antifungal Candidates Containing Triazole Scaffold from Natural Rosin against Valsa mali for Crop Protection

Chen

et al. 2023

J. Agric. Food Chem.

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Two series of dehydroabietyl-1,2,4-triazole-4-Schiff-based derivatives were synthesized from rosin to control plant fungal diseases. In vitro evaluation and screening of the antifungal activity were performed using Valsa mali, Colletotrichum orbiculare, Fusarium graminearum, Sclerotinia sclerotiorum, and Gaeumannomyces graminis. Compound 3f showed excellent fungicidal activity against V. mali (EC50 = 0.537 μg/mL), which was significantly more effective than the positive control fluconazole (EC50 = 4.707 μg/mL). Compound 3f also had a considerable protective effect against V. mali (61.57%–92.16%), which was slightly lower than that of fluconazole (85.17–100%) at 25–100 μg/mL. Through physiological and biochemical analyses, the preliminary mode of action of compound 3f against V. mali was explored. Ultrastructural observation of mycelia showed that compound 3f hindered the growth of the mycelium and destroyed the ultrastructure of V. mali seriously. Conductivity analysis and laser scanning confocal microscope staining showed that compound 3f changed cell-membrane permeability and caused accumulation of reactive oxygen species. The enzyme activity results showed that compound 3f significantly inhibited the activity of CYP51 (59.70%), SOD (76.9%), and CAT (67.86%). Molecular docking identified strong interaction energy between compound 3f and crystal structures of CYP51 (−11.18 kcal/mol), SOD (−9.25 kcal/mol), and CAT (−8.79 kcal/mol). These results provide guidance for the discovery of natural product-based antifungal pesticide candidates.

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Section: Resultsmentioning

confidence: 99%

Section: N T N Inhibition Rate (%) (mentioning

confidence: 99%

Design and Synthesis of Antifungal Candidates Containing Triazole Scaffold from Natural Rosin against Valsa mali for Crop Protection

Chen

et al. 2023

J. Agric. Food Chem.

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Detailed evaluation of a cellular in vitro method to assess oxidative potential of atmospheric aerosols as performed by two independent laboratories

Fang

Antkiewicz

Huang

et al. 2022

Atmospheric Pollution Research

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Characteristics and Oxidative Potential of Ambient PM2.5 in the Yangtze River Delta Region: Pollution Level and Source Apportionment

et al. 2023

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Fine particulate matter (PM2.5) is a major contributor to the degree of air pollution, and it is associated with a range of adverse health impacts. Moreover, the oxidative potential (OP, as a tracer of oxidative stress) of PM2.5 has been thought to be a possible determinant of its health impact. In this study, the OP of 136 fine aerosol filter samples collected in Changzhou in two seasons (spring and summer) were determined using a dithiothreitol (DTT) assay. Source apportionments of the PM2.5 and DTT activity were further performed. Our results showed that the daily average ± standard deviation of the DTTv (volume-normalized DTT activity) in the PM2.5 was 1.16 ± 0.58 nmol/min/m3 and 0.85 ± 0.16 nmol/min/m3 in the spring and summer, respectively, and the DTTm (mass-normalized DTT activity) was 13.56 ± 5.45 pmol/min/μg and 19.97 ± 6.54 pmol/min/μg in the spring and summer, respectively. The DTTv was higher in the spring compared to the summer while the opposite was true for the DTTm. Most of the detected components (including the organic component, element component, NH4+, Mn, Cu, Zn, etc.) exhibited a moderately positive correlation with the DTTv, but the opposite was found with the DTTm. An aerodyne high-resolution aerosol mass spectrometer (HP-AMS) was deployed to probe the chemical properties of the water-soluble organic matter (WSOA). Positive matrix factorization (PMF) coupled with multiple linear regression was used to obtain the relative source contributions to the DTT activity for the WSOA in the PM2.5. The results showed that the sensitivity sequences of the DTTv to the WSOA sources were oxygenated organic aerosol (OOA) > biomass burning OA (BBOA) > hydrocarbon-like OA (HOA) in the spring and HOA > nitrogen-enriched OA (NOA) > OOA in the summer. The PMF suggested the highest contribution from traffic emissions to the DTTv of the PM2.5 in both seasons. Our findings point to the importance of both organic components from secondary formation and transition metals to adverse health effects in this region. This study can provide an important reference for adopting appropriate public health policies regarding the detrimental outcomes of exposure to PM2.5.

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Reactive oxygen species (ROS) activity of fine particulate matter health impacts in Addis Ababa, Ethiopia

Cited by 3 publications

References 33 publications

Design and Synthesis of Antifungal Candidates Containing Triazole Scaffold from Natural Rosin against Valsa mali for Crop Protection

Design and Synthesis of Antifungal Candidates Containing Triazole Scaffold from Natural Rosin against Valsa mali for Crop Protection

Detailed evaluation of a cellular in vitro method to assess oxidative potential of atmospheric aerosols as performed by two independent laboratories

Characteristics and Oxidative Potential of Ambient PM2.5 in the Yangtze River Delta Region: Pollution Level and Source Apportionment

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