Reactive oxygen species (ROS) constitute an additional player in regulating epithelial development

Hebbar, Sarita; Knust, Elisabeth

doi:10.1002/bies.202100096

Cited by 16 publications

(10 citation statements)

References 131 publications

(182 reference statements)

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“…Intracellular ROS consist of a class of highly reactive chemicals, including singlet oxygen ( 1 O 2 ), hydrogen peroxide (H 2 O 2 ), superoxide (O 2 − ), hypochlorite (CIO − ), hydroxyl radical (•OH), and peroxynitrite (ONOO − ) [18]. ROS play an important role in normal physiology and maintaining homeostasis in cells; however, excessive ROS can induce oxidative stress, which is defined as the disequilibrium of the pro-/antioxidant balance and causes mitochondrial damage [19][20][21]. According to various studies, oxidative stress is related to the pathophysiology of inflammation, cancer, fibrosis, apoptosis, and reperfusion injury [22].…”

Section: Introductionmentioning

confidence: 99%

Pyroptosis-Mediated Damage Mechanism by Deoxynivalenol in Porcine Small Intestinal Epithelial Cells

Kang

Shin

Park

et al. 2023

Toxins

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Deoxynivalenol (DON) is known as a vomitoxin, which frequently contaminates feedstuffs, such as corn, wheat, and barley. Intake of DON-contaminated feed has been known to cause undesirable effects, including diarrhea, emesis, reduced feed intake, nutrient malabsorption, weight loss, and delay in growth, in livestock. However, the molecular mechanism of DON-induced damage of the intestinal epithelium requires further investigation. Treatment with DON triggered ROS in IPEC-J2 cells and increased the mRNA and protein expression levels of thioredoxin interacting protein (TXNIP). To investigate the activation of the inflammasome, we confirmed the mRNA and protein expression levels of the NLR family pyrin domain containing 3 (NLRP3), apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC), and caspase-1 (CASP-1). Moreover, we confirmed that caspase mediates the mature form of interleukin-18, and the cleaved form of Gasdermin D (GSDMD) was increased. Based on these results, our study suggests that DON can induce damage through oxidative stress and pyroptosis in the epithelial cells of the porcine small intestine via NLRP3 inflammasome.

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Section: Introductionmentioning

confidence: 99%

Pyroptosis-Mediated Damage Mechanism by Deoxynivalenol in Porcine Small Intestinal Epithelial Cells

Kang

Shin

Park

et al. 2023

Toxins

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“…•− ), hydroxyl radical (•OH), and singlet oxygen ( 1 O 2 ), are closely related to various cellular activities, such as physiological regulation and tissue growth. 98 During wound healing, low concentrations of ROS can act as intracellular messengers to regulate relevant pathways and promote wound repair. However, excessive ROS can cause oxidative damage to cells, increase the release of proinflammatory factors, and induce inflammation, thereby delaying wound healing.…”

Section: Pod-like Activity Ros Mainly Hydrogen Peroxide Superoxide An...mentioning

confidence: 99%

Nanozymes for the Therapeutic Treatment of Diabetic Foot Ulcers

Xiao,

Zhao,

DuBois

et al. 2024

ACS Biomater. Sci. Eng.

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Diabetic foot ulcers (DFU) are chronic, refractory wounds caused by diabetic neuropathy, vascular disease, and bacterial infection, and have become one of the most serious and persistent complications of diabetes mellitus because of their high incidence and difficulty in healing. Its malignancy results from a complex microenvironment that includes a series of unfriendly physiological states secondary to hyperglycemia, such as recurrent infections, excessive oxidative stress, persistent inflammation, and ischemia and hypoxia. However, current common clinical treatments, such as antibiotic therapy, insulin therapy, surgical debridement, and conventional wound dressings all have drawbacks, and suboptimal outcomes exacerbate the financial and physical burdens of diabetic patients. Therefore, development of new, effective and affordable treatments for DFU represents a top priority to improve the quality of life of diabetic patients. In recent years, nanozymes-based diabetic wound therapy systems have been attracting extensive interest by integrating the unique advantages of nanomaterials and natural enzymes. Compared with natural enzymes, nanozymes possess more stable catalytic activity, lower production cost and greater maneuverability. Remarkably, many nanozymes possess multienzyme activities that can cascade multiple enzymecatalyzed reactions simultaneously throughout the recovery process of DFU. Additionally, their favorable photothermal-acoustic properties can be exploited for further enhancement of the therapeutic effects. In this review we first describe the characteristic pathological microenvironment of DFU, then discuss the therapeutic mechanisms and applications of nanozymes in DFU healing, and finally, highlight the challenges and perspectives of nanozyme development for DFU treatment.

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“…Reactive oxygen species (ROS) play a pivotal role in biological processes through the regulation of various cellular signaling pathways. Continuous ROS production in normal cells is tightly regulated by antioxidants [30]. ROS exist in two forms: The first comprises free oxygen radicals (e.g., superoxide, hydroxyl radicals, nitric oxide, alkoxyl radicals, and peroxyl radicals).…”

Section: Source Of Hybrid Moleculesmentioning

confidence: 99%

Colorectal Cancer Chemoprevention by S-Allyl Cysteine–Caffeic Acid Hybrids: In Vitro Biological Activity and In Silico Studies

et al. 2022

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Conventional chemotherapy for colorectal cancer (CRC) gives only a small increase in patient survival, since it is often diagnosed at late stages, when the tumor has disseminated to other organs. Moreover, it is common to observe that malignant cells may acquire resistance to conventional chemotherapies through different mechanisms, including reducing drug activation or accumulation (by enhancing efflux), inducing alterations in molecular targets, and inhibiting the DNA damage response, among other strategies. Considering these facts, the discovery of new molecules with therapeutic potential has become an invaluable tool in chemoprevention. In this context, we previously evaluated two hybrids (SAC-CAFA-MET and SAC-CAFA-PENT) that exhibited selective cytotoxicity against SW480 cells, with better results than the conventional chemotherapeutic agent (5-fluorouracil; 5-FU). Here, we investigated the possible mechanisms of these molecules in greater depth, to identify whether they could be valuable therapeutic scaffolds in the search for new molecules with chemopreventive potential for the treatment of CRC. Both compounds reduced ROS formation, which could be related to antioxidant effects. Further evaluations showed that SAC-CAFA-MET induces cell death independent of caspases and the tumor-suppressor protein p53, but probably mediated by the negative regulation of the pro-apoptotic Bcl-2. In addition, the lack of activation of caspase-8 and the positive regulation of caspase-3 induced by SAC-CAFA-PENT suggest that this compound acts through an apoptotic mechanism, probably initiated by intrinsic pathways. Furthermore, the downregulation of IL-6 by SAC-CAFA-PENT suggests that it also induces a significant anti-inflammatory process. In addition, docking studies would suggest caspase-3 modulation as the primary mechanism by which SAC-CAFA-PENT elicits apoptosis in SW480human colorectal adenocarcinoma cells. Meanwhile, density functional theory (DFT) calculations suggest that both hybrids would produce effects in the modulation of ROS in SW480 cells via the hydrogen atom transfer (HAT) pathway. The present work notes that SAC-CAFA-MET and SAC-CAFA-PENT could be potential candidates for further investigations in the search for potential chemopreventive agents.

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Reactive oxygen species (ROS) constitute an additional player in regulating epithelial development

Cited by 16 publications

References 131 publications

Pyroptosis-Mediated Damage Mechanism by Deoxynivalenol in Porcine Small Intestinal Epithelial Cells

Pyroptosis-Mediated Damage Mechanism by Deoxynivalenol in Porcine Small Intestinal Epithelial Cells

Nanozymes for the Therapeutic Treatment of Diabetic Foot Ulcers

Colorectal Cancer Chemoprevention by S-Allyl Cysteine–Caffeic Acid Hybrids: In Vitro Biological Activity and In Silico Studies

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