Reactive Oxygen Species—(ROS) Pathogens or Sources of Vital Energy? Part 1. ROS in Normal and Pathologic Physiology of Living Systems

Воейков, В.Л.

doi:10.1089/acm.2006.12.111

Cited by 29 publications

(19 citation statements)

References 59 publications

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“…Recent estimations have shown that in adults >15% of oxygen consumed is turned into ROS under resting conditions and much more when their activity is high [8]. The process of one-electron oxygen reduction, in which energy of electronic excitation is generated, continuously occurs in human blood [9].…”

Section: Discussionmentioning

confidence: 99%

Changes in chemiluminescence of whole blood of COPD patients treated with Hypoxen® and effects of C60 fullerenes on blood chemiluminescence

et al. 2012

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SummaryBackgroundChronic obstructive pulmonary disease (COPD) is an inflammatory disease associated with reactive oxygen species (ROS) production. The aim of this study was to evaluate the effect of Hypoxen® treatment and the effect of HyFnC60 on ROS production in patients’ blood.Material/MethodsROS production in blood was estimated using chemiluminescence (CL) measurement with CL-amplifiers: luminol (LM), LM + zymosan (ZM) or lucigenin (LC) in the presence or absence of hydrated fullerenes (HyFnC60) added to blood in low concentrations.ResultsIn all the patients with COPD in remission phase with Hypoxen® prescription, the LM-dependent CL (LM-CL) with ZM and LC-enhanced CL (LC-CL) decreased after the treatment. Parameters of CL and effects of HyFnC60 upon them depended on blood state. Addition of HyFnC60 to blood decreased data scattering and helped to improve discrimination between different groups of patients. Using the discriminator analysis, we found the most important time-points in the kinetic curves of CL for classification of patients into groups (eg, COPD patients before and after treatment with Hypoxen®; patients’ blood with different sensitivity to HyFnC60 concentration).ConclusionsMonitoring of CL of non-diluted whole blood in COPD patients can be used for the estimation of the Hypoxen® efficiency in complex therapy. Addition of HyFnC60 to blood increases sensitivity of the method.

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Section: Discussionmentioning

confidence: 99%

Changes in chemiluminescence of whole blood of COPD patients treated with Hypoxen® and effects of C60 fullerenes on blood chemiluminescence

et al. 2012

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“…However, besides the negative effects, ROS are also actively produced and required for many physiological functions in cells (Stone and Yang, 2006;Voeikov, 2006). In addition to the role of ROS in pathogen killing and angiogenesis (Segal, 2005;Ushio-Fukai, 2006), the production of ROS has also been reported to be important in cellular stress conditions.…”

Section: Discussionmentioning

confidence: 99%

The Rac activator Tiam1 prevents keratinocyte apoptosis by controlling ROS-mediated ERK phosphorylation

Rygiel

Mertens

Strumane

et al. 2008

Journal of Cell Science

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Tiam1 is a ubiquitously expressed activator of the small GTPase Rac. Previously, we found that Tiam1 knockout (KO) mice are resistant to DMBA-induced skin tumorigenicity, which correlated with increased apoptosis in keratinocytes of the skin epidermis. Here, we have studied the mechanisms by which Tiam1 protects against apoptosis. We found that Tiam1-KO keratinocytes show increased apoptosis in response to apoptotic stimuli, including growth factor deprivation and heat-shock treatment. Expression of catalytically active Tiam1, but not inactive Tiam1, rescues the apoptosis susceptibility of Tiam1-KO keratinocytes, indicating that this defect is caused by impaired Tiam1-mediated Rac activation. Apoptosis induced by growth factor starvation correlates with impaired ERK phosphorylation in Tiam1-KO keratinocytes. Moreover, Tiam1-KO keratinocytes contain lower levels of intracellular reactive oxygen species (ROS) when compared with wild-type cells. The ROS content of keratinocytes is dependent on both Tiam1 and the activity of NADPH oxidase (Nox), and is required for ERKmediated survival signaling. Indeed, Tiam1 deficiency or the inhibition of intracellular ROS production blocks ERK phosphorylation and sensitizes wild-type keratinocytes to apoptotic stimuli. Our results indicate that the Rac activator Tiam1 controls the intracellular redox balance by Nox-mediated ROS production, which regulates ERK phosphorylation and the susceptibility of keratinocytes to apoptotic signaling.

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“…But in the last forty years, evidences indicated increasingly Janus-faced behaviors of this element1-3 for the following reasons: Under certain conditions, oxygen may produce reactive species, even free radicals responsible for different molecular cell response like cellular stress4,5. Despite all undesired consequences provoked by these oxygen's properties, these facts were not yet in the focus of the scientific discussion and still poorly understood during the last few years as illustrated comprehensively6.…”

Section: Introductionmentioning

confidence: 99%

Autofluorescent Proteins as Photosensitizer in Eukaryontes

Waldeck¹,

Mueller²,

Wießler³

et al. 2009

Int. J. Med. Sci.

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Since the discovery of the green fluorescent green protein (GFP) in 1961 many variants of fluorescent proteins (FP) were detected. The importance was underlined by the Nobel price award in chemistry 2008 for the invention, application, and development of the GFP by Shimomura, Chalfie and Tsien. GFP, first described by Shimomura now is indispensible in the scientific daily life.Since then and also in future fluorescent proteins will lead to new applications as reporters in cell biology. Such FPs can absorb visible day-light and predominantly one variant of the red fluorescent protein, the KillerRed protein (KRED) emits active electrons producing reactive oxygen species (ROS) leading to photokilling processes in eukaryotes. KRED can be activated by daylight as a photosensitizing agent. It is quite obvious that the KRED's expression and localization is critical with respect to damage, mutation and finally killing of eukaryotic cells. We found evidence that the KRED's cytotoxicity is ascendantly location-dependent from the cell membrane over the nuclear lamina to the chromatin in the cell nucleus. Daylight illumination of cells harbouring the KRED protein fused with the histone H2A, a DNA-binding protein which is critical for the formation of the chromatin structure results in cell killing. Therefore the H2A-KRED fusion protein can be considered as an appropriate candidate for the photodynamic therapy (PDT). This finding can be transferred to current photodynamic approaches and can enhance their therapeutic outcome.

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Reactive Oxygen Species—(ROS) Pathogens or Sources of Vital Energy? Part 1. ROS in Normal and Pathologic Physiology of Living Systems

Cited by 29 publications

References 59 publications

Changes in chemiluminescence of whole blood of COPD patients treated with Hypoxen® and effects of C60 fullerenes on blood chemiluminescence

Changes in chemiluminescence of whole blood of COPD patients treated with Hypoxen® and effects of C60 fullerenes on blood chemiluminescence

The Rac activator Tiam1 prevents keratinocyte apoptosis by controlling ROS-mediated ERK phosphorylation

Autofluorescent Proteins as Photosensitizer in Eukaryontes

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