Electric vehicles (EVs) have been receiving greater attention as a tool for frequency control due to their fast regulation capability. The proliferation of EVs for primary frequency regulation is hampered by the need to simultaneously maintain industrial microgrids dispatch and EV state of charge levels. The current research aims to examine the operative and dominating role of the charging station operator, along with a vehicle to grid strategy; where, indeterminate tasks are executed in the microgrid without the EVs charging/discharging statistics. The role of the charging station operator in regulation is the assignment of the job inside the primary frequency control capacity of electric vehicles. Real-time rectification of programmed vehicle to grid (V2G) power ensures electric vehicles’ state of charge at the desired levels. The proposed V2G strategy for primary frequency control is validated through the application of a two-area interconnected industrial micro-grid and another microgrids with renewable resources. Regulation specifications are communicated to electric vehicles and charging station operators through an electric vehicle aggregator in the proposed strategy. At the charging station operator, V2G power at the present time is utilized for frequency regulation capacity calculation. Subsequently, the V2G power is dispatched in light of the charging demand and the frequency regulation. Furthermore, V2G control strategies for distribution of regulation requirement to individual EVs are also developed. In summary, the article presents a novel primary frequency control through V2G strategy in an industrial microgrid, involving effective coordination of the charging station operator, EV aggregator, and EV operator.