Reactivity in the FTA-ABS test of rabbits hyperimmunized with nonpathogenic treponemes.

Tringali, Giuseppe; Cox, Patricia M.

doi:10.1136/sti.46.4.313

Cited by 4 publications

(3 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Animals inoculated subcutaneously with the supernatant antigen complex produced antibodies reactive in the VDRL and FTA-ABS tests. This finding is an agreement with that of Deacon and Hunter (1962), Kiraly, JobbaIgy and Kovats (1967), Meyer and Hunter (1967), and Tringali and Cox (1970), who have reported the existence of common antigens between cultivatable and pathogenic treponemes. The fact that the VDRL antibodies were induced both in animals immunized with the supernatant and in animals immunized with the medium suggests the presence of a nonspecific antigen in the medium.…”

Section: Discussionsupporting

confidence: 92%

Immunological studies on treponemal antigens. II. Serological changes and resistance to infection in rabbits immunized with culture supernatant of avirulent Treponema pallidum.

Izzat¹,

Smith²,

Jackson³

et al. 1971

Sexually Transmitted Infections

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 92%

Immunological studies on treponemal antigens. II. Serological changes and resistance to infection in rabbits immunized with culture supernatant of avirulent Treponema pallidum.

Izzat¹,

Smith²,

Jackson³

et al. 1971

Sexually Transmitted Infections

View full text Add to dashboard Cite

“…The FTA-ABS test is a standard confirmatory test for treponemal infection, but there is no doubt that the common group antigen which occurs in a variety of spirochaetes (Deacon and Hunter, 1962) can produce antibody in experimental animals which is detected by the FTA test (Tringali and Cox, 1970). Therefore, antibody to commensal spirochaetes could account for some anomalous false positive results in human sera.…”

Section: Discussionmentioning

confidence: 99%

Syphilitic immunofluorescence in experimental relapsing fever.

Wright¹,

Ginger²

1973

Sexually Transmitted Infections

View full text Add to dashboard Cite

Immunological cross-reactivity has been shown between related genera of the family Treponemataceae. Extracts of Borrelia cross-react with antibodies in syphilitic serum (Saurino and De Lamater, 1952) and produce positive skin tests in the later stages of syphilis (Ranque, Quilici, and Assadourian, 1967 (1962). Both techniques involved preliminary fixation in acetone and then through graded alcohols with final fixation in methanol. Sera were taken in 5 ,ul. amounts, either undiluted or as reciprocal dilutions of 5, 25, 125, and 625 in phosphate buffered saline. Specific goat anti-mouse globulin conjugated with fluorescein isothiocyanate (Hyland) was used at a dilution of 1/80. Results were recorded as negative (-), equivocal (±), weak positive (wk.+), and positive (+). There was no differentiation between + + + and + + + + as used in the routine FTA-ABS test. The microscope used was a 'Zetopan' (Reichert) with an HB 200 high pressure mercury vapour lamp. All tests were carried out in duplicate, and read independently by two observers, concordance being required for assessment of results as positive. ABSORPTION TESTSThe effects of absorption with the 'sorbent' (Burroughs Wellcome) used in the FTA absorption test and Reiter protein (Burroughs Wellcome) were tested on sera from mice experimentally infected with B. duttoni and T. pallidum. Indirect immunofluorescence was then carried out on Borrelia and T. pallidum substrates. The absorbing potency of the sorbent was assessed using human nonspecific positive control sera. These were tested at dilutions of 1 in 5, 1 in 25, and 1 in 125 in saline and in sorbent against T. pallidum and Reiter antigens.

show abstract

“…It has been suggested (Tringali and Cox, 1970) that low-titre fluorescent treponemal antibodies found in normal sera could be due to commensal spirochaetes. In relapsing fever in man (Alline and Marx, 1966) and experimental animals (Wright and Ginger, 1973) a positive fluorescent treponemal antibody is produced.…”

Section: Discussionmentioning

confidence: 99%

The significance of the fluorescent treponemal antibody (FTA-ABS) test in collagen disorders and leprosy

Wright

1973

J Clin Pathol

View full text Add to dashboard Cite

SYNOPSIS A test is described to distinguish the immunofluorescence seen on T. pallidum in syphilis from that due to antibodies against DNA.The method consists of comparing the results of the fluorescent treponemal antibody (FTA-ABS) test with the staining obtained with T. gondii and T. cruzi using an indirect fluorescent technique. The ability of a particular serum to stain other organisms, in addition to T. pallidum, at a significant titre indicates that the treponemal fluorescence is not specific, and the loss of this staining property when organisms are pretreated with deoxyribonuclease suggests that the common microbial antigen is DNA. The isolated treponemal fluorescence found in syphilis is unaffected by pretreatment of T. pallidum.When 123 sera from patients with collagen disorders were examined, non-specific bacterial fluorescence was observed in six sera, while in 95 sera from patients with lepromatous leprosy, a positive FTA-ABS reaction was obtained in 14. There was no accompanying immunofluorescence on other protozoa. Therefore the antibody reacting in the FTA-ABS test in leprosy is likely to be treponemal in origin. A mitochondrial antibody was found in seven out of the 15 sera from patients with lepromatous leprosy.Positive fluorescent treponemal antibody tests may rarely occur in diseases other than syphilis, in particular, in collagen disorders (Kraus, Haserick, and Lantz, 1970). Scotti, Mackey, and Trautman (1970) have suggested that the immunofluorescent treponemal reaction seen in leprosy is akin to that found in collagen disorders and may be caused by a common aetiological factor. Origin of Test SeraTwo hundred and seventy-eight sera were studied;

show abstract

Reactivity in the FTA-ABS test of rabbits hyperimmunized with nonpathogenic treponemes.

Cited by 4 publications

References 8 publications

Immunological studies on treponemal antigens. II. Serological changes and resistance to infection in rabbits immunized with culture supernatant of avirulent Treponema pallidum.

Immunological studies on treponemal antigens. II. Serological changes and resistance to infection in rabbits immunized with culture supernatant of avirulent Treponema pallidum.

Syphilitic immunofluorescence in experimental relapsing fever.

The significance of the fluorescent treponemal antibody (FTA-ABS) test in collagen disorders and leprosy

Contact Info

Product

Resources

About