Reactivity of fungal organisms in tissue sections using anti‐mycobacteria antibodies

Wiley, Elizabeth L.; Beck, Billie; Freeman, Robert G.

doi:10.1111/j.1600-0560.1991.tb00154.x

Cited by 23 publications

(19 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Septate hyphae were revealed by both periodic acid-Schiff staining (PAS) and Gomori methamine staining (GMS) inside and outside the macrophages. Immunohistochemistry was performed by the avidin-biotin-peroxidase method (3,22). Antibodies are listed in Table 1.…”

Section: Case Reportmentioning

confidence: 99%

Deep Infection by Trichophyton rubrum in an Immunocompromised Patient

Nir-Paz¹,

Elinav²,

Pierard

et al. 2003

J Clin Microbiol

View full text Add to dashboard Cite

Dermatophytes are common pathogens of skin but rarely cause invasive disease. We present a case of deep infection by Trichophyton rubrum in an immunocompromised patient. T. rubrum was identified by morphological characteristics and confirmed by PCR. Invasiveness was apparent by histopathology and immunohistochemistry. The patient was treated successfully with itraconazole. CASE REPORTA 56-year-old male was admitted for evaluation of elevated erythrocyte sedimentation rate (ESR), anemia, and multiple subcutaneous nodules on both legs. The patient was under close medical supervision due to an autoimmune disease with liver, cardiac, and lung involvement.His medical history included pericarditis (27 years before admission), and a profound jaundice after a respiratory infection treated with cefuroxime (3 years before admission). Serology was negative for hepatitis viruses and positive for antiparietal, antinuclear, and antineutrophil cytoplasmic antibodies. Liver biopsy revealed a profound destruction of liver architecture, fibrosis, active inflammation, and cholestasis. Treatment with steroids was followed by a good clinical response and normalization of liver enzymes. Attempts to wean the patient from steroids including administration of azathioprine and cyclosporine failed, and during the 2 years prior to admission he received both prednisone and cyclosporine. Several weeks before admission, he was evaluated for a chronic cough. He had undergone a transbronchial biopsy that disclosed chronic inflammation and thickening of the basement membrane, without any specific diagnosis, but responded to an increase of the dose of steroids. A few weeks later, while trying to taper the steroids, he developed multiple, hard cutaneous nodules, distributed mainly on the lower limbs. Some later softened and discharged caseous material spontaneously.Other underlying conditions included glucose-6-phosphate dehydrogenase deficiency, nephrolithiasis, benign prostate hypertrophy, bilateral inguinal hernia repair, and osteoporosis.On admission, medications included prednisone (10 mg once a day [QD]), cyclosporine (100 mg QD), ursodeoxycholic acid (300 mg twice a day), alendronate (10 mg QD), omeprazole (20 mg QD), and calcium (600 mg) and vitamin D (0.25 g) once daily. On physical examination, rhonchi were heard over both lungs. A few hard, mobile subcutaneous nodules were present on both lower limbs, mainly around the knees and thighs (Fig. 1a), and the sacral area. Some of these nodules were purplish and soft. Onychomycosis was present on the feet and both hands (Fig. 1b and c).Laboratory test results were as follows: ESR, 80; hemoglobin level, 10.8 g/dl; leukocyte count, 7,900/l (42% granulocytes); albumin level, 34 g/liter; total protein level, 83 g/liter; liver enzyme levels, normal.Two of the patient's nodules were excised. A granulomatous inflammatory reaction was present in the dermis and hypodermis. It was composed of monocytes, macrophages, multinucleated giant cells, and rare neutrophils (Fig. 2). Septate hyphae were revealed...

show abstract

Section: Case Reportmentioning

confidence: 99%

Deep Infection by Trichophyton rubrum in an Immunocompromised Patient

Nir-Paz¹,

Elinav²,

Pierard

et al. 2003

J Clin Microbiol

View full text Add to dashboard Cite

show abstract

“…The parallel use of different histochemical staining methods (HSM) is time consuming and expensive. There are some reports about the successful application of rabbit polyclonal antibody directed against the Bacillus Calmette-Guérin (BCG) strain of Mycobacterium bovis in the immunohistochemical detection (IHC) of various bacteria and fungi in skin biopsies (Wiley et al, 1991;Kutzner et al, 1998;Bonenberger et al, 2001). Studies in human medicine have shown that polyclonal anti-BCG antibodies cross-react with a broad spectrum of bacteria, fungi and also with a few parasites (Minden et al, 1972;Harboe et al, 1979;Thorns and Morris, 1986).…”

mentioning

confidence: 99%

“…Epitopes of these microorganisms were identified by monoclonal antibodies as being similar to epitopes from mycobacterial antigens (Thorns and Morris, 1986). In the latter investigations anti-BCG antibodies were successfully used for immunohistochemical detection of the most common cutaneous microbial pathogens in formalin-fixed and paraffin-embedded human tissue samples (Wiley et al, 1991;Kutzner et al, 1998). This method was found to be superior to conventional HSM in terms of quality and sensitivity, and it was helpful especially in those cases where only a few organisms were present, obscured by a dense overlying inflammatory infiltrate (Kutzner et al, 1998).…”

mentioning

confidence: 99%

Application of anti-BCG antibody for rapid immunohistochemical detection of bacteria, fungi and protozoa in formalin-fixed paraffin-embedded tissue samples

Szeredi

Gianni

Tenk

et al. 2008

Acta Veterinaria Hungarica

View full text Add to dashboard Cite

The applicability of an anti-Mycobacterium bovis (BCG) antibody-based immunohistochemistry (IHC) procedure was investigated using everyday veterinary pathological samples collected from 13 different animal species. Fifty-one formalin-fixed and paraffin-embedded tissue samples were selected for this study. Forty, 4 and 7 tissue samples contained different species of bacteria, fungi and protozoa, respectively. Three serial sections were prepared in each case. Two sections were pre-treated with enzyme and heat, respectively, while the last section was not pre-treated. In seven cases the sensitivity of histochemical staining (HSM), IHC and bacteriological culture were compared. Heating of the sections in a microwave oven was the most effective method in the case of almost all pathogens used. Strong or moderate positive reactions were observed for 26 bacterial species, all fungal and 2 protozoal species, while weak reactions occurred for 2 bacterial and 1 protozoal species. Only 4 protozoal and 12 bacterial species, including Leptospira and all the five Mycoplasma species examined, showed no reaction in this test. IHC had almost the same sensitivity as bacteriological culture and was more sensitive than HSM. The IHC method presented here should be preferred to HSM as a general screening tool in cases where pathological lesions suspicious for infections are evident and no microorganism can be cultured in vitro or only formalin-fixed tissue samples are available for the laboratory examination.

show abstract

“…Nesta situação, a coloração específica para bacilos álcool-ácido-resistentes (BAAR), métodos de imunohistoquímica e teste tuberculínico são exames importantes para o diagnóstico diferencial. Com freqüência observa-se em formas paucibacilares da doença, que a pesquisa de BAAR, a imunohistoquímica e as culturas são negativas (Hsu et al, 1981;Wiley et al, 1991;Taippeiner, Wolf, 1993;Kox, 1995;Kutzner et al, 1998;Bollela et al, 1999;Ulukanligil et al, 2000;Ferreira et al, 2002).…”

Section: Objetivosunclassified

“…No caso da micobactéria, desenvolveu-se anticorpo primário de imunoglobulina de coelho anti-Mycobacterium bovis (anti-BCG (Beck, Freeman, 1991;Whiley et al, 1991;Kutzner et al, 1998).…”

Section: Imunohistoquímicaunclassified

"Avaliação crítica do uso da reação em cadeia da polimerase e exames complementares no diagnóstico da tuberculose cutânea e micobacteriose atípica"

Abdalla¹

View full text Add to dashboard Cite

Reactivity of fungal organisms in tissue sections using anti‐mycobacteria antibodies

Cited by 23 publications

References 8 publications

Deep Infection by Trichophyton rubrum in an Immunocompromised Patient

Deep Infection by Trichophyton rubrum in an Immunocompromised Patient

Application of anti-BCG antibody for rapid immunohistochemical detection of bacteria, fungi and protozoa in formalin-fixed paraffin-embedded tissue samples

"Avaliação crítica do uso da reação em cadeia da polimerase e exames complementares no diagnóstico da tuberculose cutânea e micobacteriose atípica"

Contact Info

Product

Resources

About