Reactivity of Monoclonal Antibodies Specific for H‐2 Antigenic Determinants with Cells of Wild Mice

Tracheal transplants were performed on inbred strains of rats. Monoclonal antibodies were used in combination with the biotin-avidin technique for demonstrating donor-specific RT1 Ac transplantation antigen. Transplantation antigens were predominantly contained in the tracheal mucosal cells. No donor-specific antigens could be found in the mucosa of transplants which survived orthotopic tracheal transplantation for longer than 210 days. This suggests that the mucosa of the transplant is rejected and replaced by a mucosa from the recipient.

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Hybridomas: A new dimension in biological analyses

Kennett

1981

In Vitro

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Since the first report of hybridomas producing monoclonal antibodies by Kohler and Milstein in 1975, this technique has spread to nearly all areas of biological, biochemical, and biomedical research. Watching the use of these methods spread from immunologists to cell biologists, developmental biologists, biochemists and to other biological disciplines and observing the nearly logarithmic increase in publications using these reagents has been in itself fascinating and informative. An overview of the development of this technology and its applications is presented including the use of monoclonal antibodies to study cell surface molecules, differentiation antigens, receptors, and histocompatibility antigens. The use of these antibodies to analyze microorganisms and parasitic antigens as well as their use in the genetic analysis of human cell surface antigens and the detection of polymorphic variation in enzymes and other proteins is discussed. Examples of the application of monoclonal reagents to the study of tumor cell biology including the labeling of metastatic tumor cells and the detection of cell surface molecules implicated in the regulation of growth control and cell division are provided.

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Reactivity of Monoclonal Antibodies Specific for H‐2 Antigenic Determinants with Cells of Wild Mice

Cited by 4 publications

References 17 publications

Trachealtransplantation beim Tier

Trachealtransplantation beim Tier

Tracheal transplantation. III. Demonstration of transplantation antigens on the tracheal mucosa of inbred rat strains

Hybridomas: A new dimension in biological analyses

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