Reactivity of monoclonal antibodies with bovine blood mononuclear cells activated by mitogens and superantigens

Assessment of Antibody‐independent Cellular Cytotoxicity (AICC) of Porcine Neutrophilic Granulocytes by Quantitative Flow Cytometry. Lack of Modulation by Larval Products of Oesophagostomum dentatum

“…Bovine MNC were separated from heparinized blood (Schuberth et al, 1996) and labelled with a monoclonal antibody specific for bovine MHC class I molecules (mAb Bo1, Schuberth et al, 1992) and fluorescein isothiocyanate (FITC)‐conjugated goat anti‐mouse immunoglobulins (Dianova, Hamburg, Germany) according to Schuberth et al (1996). After labelling, the cells were washed in PBS and finally fixed with paraformaldehyde (4 % in PBS) for 12 h. Subsequently, the cells were washed twice in PBS and resuspended in PBS containing 5 g/l bovine serum albumin, 0.1 % NaN 3 and 2 µg/ml propidium iodide.…”

In vitro detection of antigen specific T lymphocyte subsets in cattle

“…Single color flow cytometry has been used to study changes in expression of IL2r and MHC II for mitogen activated lymphocytes from healthy cattle, as well as from animals treated with immunosuppressive doses of dexamethasone (Oldham et al, 1992) and cattle with specific infectious diseases Stone et al, 1995). Single-color analysis also has been used to study mitogen-induced changes in the percentage of ruminant lymphocyte subsets or the percentage of cells expressing surface activation markers on lymphocytes isolated from peripheral blood (Franklin et al, 1994;Davis et al, 1996b;Schuberth et al, 1996), lymph nodes (Hurley et al, 1994), and bronchoalveolar lavage fluid . Two-color flow cytometry has been used to study bovine lymphoqte subset expression of IL2r and MHC 11 in response to Con A and superantigen , and the effect of viraJ infection on bovine lymphocyte subset expression of IL2r and MHC 11 in response to mitogens (Stone et al, 1995;.…”