Reactivity with monoclonal antibodies of viruses from an episode of foot-and-mouth disease

Mateu, Mauricio G.; Rocha, E; Vicente, Óscar; Vayreda, F.; C, Navalpotro; Andreu, David; Pedroso, Enrique; Giralt, Ernest; Enjuanes, Luis; Domingo, Esteban

doi:10.1016/0168-1702(87)90020-7

Cited by 112 publications

(103 citation statements)

References 30 publications

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“…5b; green lines). As expected, neither DMEM with FCS nor the neutralizing activity of mAb SD6 (specific for FMDV; described in Mateu et al, 1987) was affected by the same doses of UV irradiation. These results exclude proteins or small molecules as the interfering component.…”

Section: Stochastic Lcmv Extinction In Bhk-21 Cells Interference Exementioning

confidence: 77%

An interfering activity against lymphocytic choriomeningitis virus replication associated with enhanced mutagenesis

Martı́n

Abia

Domingo

et al. 2009

Journal of General Virology

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Previous studies have documented that, in the presence of the mutagenic base analogue 5-fluorouracil (FU), lymphocytic choriomeningitis virus (LCMV) that persisted in BHK-21 cells decreased its infectivity to a larger extent than intracellular viral RNA levels, prior to virus extinction. This observation, together with in silico simulations, led to the proposal of the lethal defection model of virus extinction. This model suggests the participation of defective-interfering genomes in the loss of infectivity by increased mutagenesis. Since LCMV naturally produces defective-interfering particles, it was important to show that a capacity to interfere is produced in association with FU treatment. Here, we document that BHK-21 cells persistently infected with LCMV grown in the presence of FU, but not in its absence, generated an interfering activity that suppressed LCMV infectivity. Interference was specific for LCMV and was sensitive to UV irradiation and its activity was dose-and time-dependent. The interfering preparations produced positive LCMV immunofluorescence and viral particles seen by electron microscopy when used to infect cells, despite some preparations being devoid of detectable infectivity. Interference did not involve significant increases of mutant spectrum complexity, as predicted by the lethal defection model. The results provide support for a specific interference associated with LCMV when the virus replicates in the presence of FU. The excess of interference relative to that observed in the absence of FU is necessary for LCMV extinction.

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Section: Stochastic Lcmv Extinction In Bhk-21 Cells Interference Exementioning

confidence: 77%

An interfering activity against lymphocytic choriomeningitis virus replication associated with enhanced mutagenesis

Martı́n

Abia

Domingo

et al. 2009

Journal of General Virology

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“…Using the crystallographic coordinates of FMDV C-S8c1 (17), the substitutions found in the capsid of FMDV C-S8c1p100, mutant (Asp-143-Gly) (see Table 2), have been modeled with the program TURBO (32) by placing the side chain atoms of the substituted amino acids in their standard conformations. The model was then optimized by energy minimization using the program X-PLOR (33).…”

Section: Methodsmentioning

confidence: 99%

“…mAb SD6, which recognizes a continuous epitope located within amino acids 136-147 of VP1 of FMDV C-S8c1 (23,29,32,33), was used to isolate resistant mutants. Viruses C-S8c1 and its derivative C-S8c1p100, obtained by propagating C-S8c1 in cell culture (see Materials and Methods) were incubated with mAb SD6 and resistant mutants were selected.…”

Section: Overcoming Restrictions To Variation: a New Repertoire Ofmentioning

confidence: 99%

Evolution subverting essentiality: Dispensability of the cell attachment Arg-Gly-Asp motif in multiply passaged foot-and-mouth disease virus

Martı́nez

Verdaguer

Mateu

et al. 1997

Proc. Natl. Acad. Sci. U.S.A.

119

117

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Aphthoviruses use a conserved Arg-Gly-Asp triplet for attachment to host cells and this motif is believed to be essential for virus viability. Here we report that this triplet-which is also a widespread motif involved in cell-tocell adhesion-can become dispensable upon short-term evolution of the virus harboring it. Foot-and-mouth disease virus (FMDV), which was multiply passaged in cell culture, showed an altered repertoire of antigenic variants resistant to a neutralizing monoclonal antibody. The altered repertoire includes variants with substitutions at the Arg-Gly-Asp motif. Mutants lacking this sequence replicated normally in cell culture and were indistinguishable from the parental virus. Studies with individual FMDV clones indicate that amino acid replacements on the capsid surface located around the loop harboring the Arg-Gly-Asp triplet may mediate in the dispensability of this motif. The results show that FMDV quasispecies evolving in a constant biological environment have the capability of rendering totally dispensable a receptor recognition motif previously invariant, and to ensure an alternative pathway for normal viral replication. Thus, variability of highly conserved motifs, even those that viruses have adapted from functional cellular motifs, can contribute to phenotypic f lexibility of RNA viruses in nature.

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“…FMDV proteins were detected using mouse MAb 6F2, which recognizes capsid protein VP2, MAb 6C2, which recognizes capsid protein VP3, MAb SD6 which recognizes capsid protein VP1 (Mateu et al, 1987), MAb 1C8 which recognizes protein 2C (kindly provided by E. Brocchi), MAb 2D2, which recognizes protein 3C (kindly provided by E. Brocchi), and rabbit polyclonal serum anti-3D (polymerase), raised against purified recombinant 3D protein, expressed in E. coli (Arias et al, 2005;Ferrer-Orta et al, 2004). Proteins were separated electrophoretically by SDS-PAGE, and transferred to a nitrocellulose membrane (Bio-Rad) in transfer buffer pH 8.3,190 mM glycine,20 % methanol,0.1 % SDS] at 200 mA for 15 h. The membrane was saturated with a solution of uncreamed powdered skimmed milk (5 %, w/v) in PBS for 1 h with gentle stirring.…”

Section: Methodsmentioning

confidence: 99%

Distance effects during polyprotein processing in the complementation between defective FMDV RNAs

Moreno

Perales

2016

Journal of General Virology

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Passage of foot-and-mouth disease virus (FMDV) in BHK-21 cells resulted in the segmentation of the viral genome into two defective RNAs lacking part of either the L-or the capsid-coding region. The two RNAs are infectious by complementation. Electroporation of L-defective RNA in BHK-21 cells resulted in the accumulation of the precursor P3 located away from the deleted sequence. Expression of L in trans led to the processing of P3, indicating that there is a connection between L protease activity and the secondary cleavages carried out by 3C protease within P3. These results suggest that the complementation mechanism between defective RNAs is not restricted to supplying the L and capsid proteins but that distance effects on polyprotein processing events are also implicated.

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Reactivity with monoclonal antibodies of viruses from an episode of foot-and-mouth disease

Cited by 112 publications

References 30 publications

An interfering activity against lymphocytic choriomeningitis virus replication associated with enhanced mutagenesis

An interfering activity against lymphocytic choriomeningitis virus replication associated with enhanced mutagenesis

Evolution subverting essentiality: Dispensability of the cell attachment Arg-Gly-Asp motif in multiply passaged foot-and-mouth disease virus

Distance effects during polyprotein processing in the complementation between defective FMDV RNAs

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