Reactor scale up for biological conversion of cellulosic biomass to ethanol

Shao, Xiongjun; Lynd, Lee R.; Bakker, A.; LaRoche, Richard D.; Wyman, Charles E.

doi:10.1007/s00449-009-0357-2

Cited by 15 publications

(4 citation statements)

References 21 publications

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“…However, largescale enzyme production from white-rot fungi is limited mainly owing to the lack of suitable bioreactor configurations for fungal growth and their metabolism [27]. Although several studies on the development of innovative bioreactor systems have already been carried out [7,25,32], an ideal bioreactor for ligninolytic enzyme production from white-rot fungi has not yet been shown [6,8]. Furthermore, the high production cost is another barrier to the commercialization of these enzymes.…”

Section: Introductionmentioning

confidence: 99%

Fungal Growth and Manganese Peroxidase Production in a Deep Tray Solid-State Bioreactor, and In Vitro Decolorization of Poly R-478 by MnP

Zhao¹,

Huang²,

Yao³

et al. 2015

Journal of Microbiology and Biotechnology

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The growth of Irpex lacteus F17 and manganese peroxidase (MnP) production in a selfdesigned tray bioreactor, operating in solid-state conditions at a laboratory scale, were studied. The bioreactor was divided into three layers by three perforated trays. Agroindustrial residues were used both as the carrier of bound mycelia and as a nutrient medium for the growth of I. lacteus F17. The maximum biomass production in the bioreactor was detected at 60 h of fermentation, which was consistent with the CO2 releasing rate by the fungus. During the stationary phase of fungal growth, the maximum MnP activity was observed, reaching 950 U/l at 84 h. Scanning electron microscopy images clearly showed the growth situation of mycelia on the support matrix. Furthermore, the MnP produced by I. lacteus F17 in the bioreactor was isolated and purified, and the internal peptide sequences were also identified with mass spectrometry. The optimal activity of the enzyme was detected at pH 7 and 25 °C, with a long half-life time of 9 days. In addition, the MnP exhibited significant stability within a broad pH range of 4-7 and at temperature up to 55 °C. Besides this, the MnP showed the ability to decolorize the polymeric model dye Poly R-478 in vitro.

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Section: Introductionmentioning

confidence: 99%

Fungal Growth and Manganese Peroxidase Production in a Deep Tray Solid-State Bioreactor, and In Vitro Decolorization of Poly R-478 by MnP

Zhao¹,

Huang²,

Yao³

et al. 2015

Journal of Microbiology and Biotechnology

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“…Standard scale-up metrics for two-phase solid-liquid mixing tanks include maintaining constant tip speed or constant power per unit volume (PV -1 ) [2]. One paper [3] studied scale-up of enzymatic hydrolysis to 2.4 million L and determined that maintaining just suspended speed (N js ) 1) is the most efficient scale-up metric, since constant tip speed does not provide sufficient agitation, while constant PV -1 consumes far too much power. This study, however, utilized waste paper sludge, uncommon in bioethanol applications, which will significantly affect rheology, and more significantly it looked at low solids concentrations of < 1 %.…”

Section: Introductionmentioning

confidence: 99%

Predicting Power for a Scaled‐up Non‐Newtonian Biomass Slurry

et al. 2014

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High-solids biomass slurries exhibit non-Newtonian behavior with a yield stress and require high power input for mixing. The goals were to determine the effect of scale and geometry on power number P 0 , and estimate the power for mixing a pretreated biomass slurry in a 3.8 million L hydrolysis reactor of conventional design. A lab-scale computational fluid dynamics model was validated against experimental data and then scaled up. A pitched-blade turbine and A310 hydrofoil were tested for various geometric arrangements. Flow was transitional; laminar and turbulence models resulted in equivalent P 0 which increased with scale. The ratio of impeller diameter to tank diameter affected P 0 for both impellers, but impeller clearance to tank diameter affected P 0 only for the A310. At least 2 MW is required to operate at this scale.

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“…CFD tools were applied to perform stirred solidliquid reactors [3]. The study of suspension of solid particles and the influence of them in the flow were considered by Shao et al [4].…”

Section: Introductionmentioning

confidence: 99%

Design optimization of a bioreactor for ethanol production using CFD simulation and genetic algorithms

Góis

Seleghim

2011

Computational Methods and Experimental Measurements XV

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The increase of the use of biofuels, like bioethanol, has presented new challenges to engineering problems. The optimization of bioreactors is crucial to improve the bioethanol extraction and to avoid stagnant zones in the flow that can compromise the chemical reactions involved in the process. This paper presents a solution using Computational Fluid Dynamics tools coupled with Genetics Algorithm to find an improved reactor structure. The preliminary results show that the influence of the height outlet tube alone is able to modify completely the flow pattern inside the reactor, improving the efficiency of the reactor.

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Reactor scale up for biological conversion of cellulosic biomass to ethanol

Cited by 15 publications

References 21 publications

Fungal Growth and Manganese Peroxidase Production in a Deep Tray Solid-State Bioreactor, and In Vitro Decolorization of Poly R-478 by MnP

Fungal Growth and Manganese Peroxidase Production in a Deep Tray Solid-State Bioreactor, and In Vitro Decolorization of Poly R-478 by MnP

Predicting Power for a Scaled‐up Non‐Newtonian Biomass Slurry

Design optimization of a bioreactor for ethanol production using CFD simulation and genetic algorithms

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