Ready-to-use DNA extracted with a CTAB method adapted for herbarium specimens and mucilaginous plant tissue

Cota-Sánchez, J. Hugo; Remarchuk, Kirsten; Ubayasena, Kumary

doi:10.1007/bf02914055

Cited by 230 publications

(154 citation statements)

References 16 publications

Supporting

Mentioning

150

Contrasting

Unclassified

Order By: Relevance

“…The ground material was then transferred to a 2-mL tube. The DNA isolation procedure used for the leaf material was a modification of the cetyltrimethylammonium bromide protocol by Cota-Sánchez et al (2006). Briefly, 500 μL of hexadecyltrimethylammonium bromide (CTAB) buffer was added to the 2-mL tube that contained the ground material.…”

Section: Dna Isolationmentioning

confidence: 99%

Molecular DNA identification of medicinal plants used by traditional healers in Malaysia

2015

View full text Add to dashboard Cite

ABSTRACT. Plants have been used throughout human history for food and medicine. However, many plants are toxic, and cannot easily be morphologically distinguished from non-toxic plants. DNA identification solves this problem and is widely used. Nonetheless, plant DNA barcode identification faces a number of challenges, and many studies have been conducted to find suitable barcodes. The present study was conducted to test the efficiency of commonly used primers, namely ITS2, rpoC1, and trnH-psbA, in order to find the best DNA barcode markers for the identification of medicinal plants in Malaysia. Fresh leaves from 12 medicinal plants that are commonly used by Malay traditional healers were collected from the Tropical Spice Garden, Pulau Pinang, and subjected to polymerase chain reaction amplification using ITS2, rpoC1, and trnH-psbA DNA markers. We found that trnH-psbA is the best DNA marker for the species-level identification of medicinal plants in Malaysia.

show abstract

Section: Dna Isolationmentioning

confidence: 99%

Molecular DNA identification of medicinal plants used by traditional healers in Malaysia

2015

View full text Add to dashboard Cite

show abstract

“…Three DNA extraction protocols proposed by Lodhi et al (1994), Keb-Llanes et al (2002), andCota-Sánchez et al (2006) were tested for recalcitrant species extraction because no specific extraction protocol existed for copao. Once the three protocols were evaluated, the protocol based on the use of cetyltrimethylammonium bromide (CTAB) detergent (Lodhi et al, 1994) was selected and specific modifications described by Cota-Sánchez et al (2006) were included; in the final stage, the pellet was resuspended in 50 µL TE buffer and incubated at 37 °C for 30 min and at 42 °C for 15 min.…”

Section: Molecular Characterization Of Copaomentioning

confidence: 99%

“…Once the three protocols were evaluated, the protocol based on the use of cetyltrimethylammonium bromide (CTAB) detergent (Lodhi et al, 1994) was selected and specific modifications described by Cota-Sánchez et al (2006) were included; in the final stage, the pellet was resuspended in 50 µL TE buffer and incubated at 37 °C for 30 min and at 42 °C for 15 min.…”

Section: Molecular Characterization Of Copaomentioning

confidence: 99%

Morphological and genetic characterization among wild populations of copao (Eulychnia acida Phil.), cactus endemic to Chile

Jana

Salvatierra

Diaz

et al. 2017

Chilean J. Agric. Res.

View full text Add to dashboard Cite

There are endemic cacti species in the North of Chile with edible fruits and a fledgling productive potential, with unknown degree diversity. Low diversity compromises the ability of populations to evolve and reduces their chances of survival under environmental changes. Copao (Eulychnia acida Phil.), a cactus endemic to Chile that grows in the Coquimbo Region (30º S lat) and able to bear fruit in water stress conditions, were morphologically characterized using descriptors of other columnar cacti at three different locations where it usually grows wild. Five inter simple sequence repeat (ISSR) primers, which were reported for other cacti that produced replicable and informative amplifications, were selected for the genetic characterization. Statistical analysis was performed to describe the diversity of this species and morphological and genetic results compared each other. High variation was observed between and within the evaluated populations for parameters of central tendency, dispersion, and univariate statistical analysis of the morphological traits. Principal component analysis showed that 73% of the population variation at the three locations could be explained by the first three principal components with 34%, 22%, and 17% of the observed variation, respectively. The five ISSR primers generated 7 to 11 polymorphic bands with a band range between 100 and 1517 bp. The polymorphic information content (PIC) averaged 0.75, which suggests high diversity between and within populations. Consensus of 85% existed between the selected descriptors and the ISSR markers, which points them out as suitable tools to analyze diversity in this species.K e y w o r d s : C o p a o , d e s c r i p t o r s , g e n e r a l i z e d procrustes analysis (GPA), genetic and morphological characterization, inter simple sequence repeat (ISSR).

show abstract

“…W770B and rye, were extracted from fresh leaves using the modified cetyltrimethylammonium bromide (CTAB) method (Cota-Sánchez et al, 2006). To improve the screening efficiency and identify rye chromatin in 13-2-2, we used five rye genome-specific molecular markers, i.e., AF1/AF4 (Francis et al, 1995), D15 (Liu et al, 2007), F3/R3 (Katto et al, 2004), Identification of a wheat-rye translocation line O5 (Jia et al, 2012), and P13LF/R (Jia and Yang, 2010) (Table 1).…”

Section: Dna Extraction and Rye Genome-specific Scar Markers Analysismentioning

confidence: 99%

Molecular cytogenetic identification of a novel 1AL.1RS translocation line with powdery mildew resistance

Lu¹,

Wang²,

Zhang³

et al. 2014

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT.A wheat germplasm line 13-2-2 with resistance to powdery mildew was isolated; this line was derived from common wheat cv. W770B and rye, Secale cereale L. (2n = 2x = 14, RR). The line was characterized based on cytological, genomic in situ hybridization (GISH), sequence-characterized amplified region (SCAR), and simple sequence repeat (SSR) analyses. The mitotic and meiotic investigations showed that the chromosome number and configuration of 13-2-2 were 2n = 42 = 21 II. GISH using rye genomic DNA as a probe detected a pair of R genome chromosome arms with strong hybridization signals in 13-2-2. Three 1RS chromosome-specific SCAR markers amplified 1RS specific bands in line 13-2-2. We screened 320 SSR primer pairs on the long or short arms from seven wheat homoeologous groups in the translocation line and parents. However, only three 1AS primers could not be amplified in line 13-2-2, whereas the others were amplified. Thus, these markers suggested that the line 13-2-2 was 1AL.1RS translocation line. Line 13-2-2 was immune to powdery Identification of a wheat-rye translocation line mildew after inoculation with Blumeria graminis f. sp. tritici isolates E05 and E07 during the adult plant stages. In contrast, the maternal parent W770B, Kavkaz with Pm8, and Amigo with Pm17 were heavily infected with spores and had reaction response scores of susceptible. Thus, the new wheat-rye 1AL.1RS translocation line with resistance to powdery mildew could be a new and valuable donor source for wheat improvement. The molecular markers developed in this study might also be useful tools for marker-assisted selection.

show abstract

Ready-to-use DNA extracted with a CTAB method adapted for herbarium specimens and mucilaginous plant tissue

Cited by 230 publications

References 16 publications

Molecular DNA identification of medicinal plants used by traditional healers in Malaysia

Molecular DNA identification of medicinal plants used by traditional healers in Malaysia

Morphological and genetic characterization among wild populations of copao (Eulychnia acida Phil.), cactus endemic to Chile

Molecular cytogenetic identification of a novel 1AL.1RS translocation line with powdery mildew resistance

Contact Info

Product

Resources

About