Reagent for the .alpha.,.beta. reduction of conjugated nitriles

Identification and characterization of a novel “thermophilic-like” Old Yellow Enzyme from the genome of Chryseobacterium sp. CA49

“…Chemicals and solvents were of analytical grade or better. Substrate 4 and corresponding racemic reduction product were synthesized as reported previously [23][24][25][26][27]. Other substrates and racemic reduction products were purchased from Sigma-Aldrich (St. Louis, MO, USA) and Alfa Aesar (Tianjin, China).…”

Synthetic Approaches to Hydroazulenes and Guaianes through [4 + 3] Cycloadditions of Oxyallyl Intermediates

“…Finally, hydrogenation of the α,β-unsaturated carbonitrile moiety was effected with magnesium in methanol (74 % yield). [24] Conversion of the saturated nitrile 8 into the methyl ketone 1 was achieved in a 59 % yield with methylmagnesium iodide in the presence of Ni(acac) 2 . [25,26] For the [4 + 3] cycloaddition of the functionalized furan 1 we generated the oxyallyl intermediate from pentachloroacetone and sodium 2,2,3,3-tetrafluoropropoxide in 2,2,3,3-tetrafluoropropan-1-ol.…”

Two “classical” Old Yellow Enzymes from Chryseobacterium sp. CA49: Broad substrate specificity of Chr-OYE1 and limited activity of Chr-OYE2

“…Chemicals and solvents were of analytical grade or better. Substrate 15a and corresponding racemic reduction product, and the racemic reduction products of 12a, 13a, and 14a were synthesized as reported previously [21][22][23][24][25][26]. Other substrates and racemic reduction products were purchased from Sigma-Aldrich (St. Louis, MO, USA) and Alfa Aesar (Tianjin, China).…”