Reagent-free mechanical patterning of gelatin surfaces by two-step electron irradiation treatment

Riedel, Stefanie; Bela, Katharina; Wisotzki, Emilia I.; Suckfüll, Carl; Zajadacz, Joachim; Mayr, Stefan

doi:10.1016/j.matdes.2018.04.076

Cited by 6 publications

(8 citation statements)

References 33 publications

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“…The macroradicals are extremely reactive and self-recombine to establish covalent bonds that build cross-links inside the polymer matrix. By comparison with chemical crosslinking techniques, electron irradiation at 5-20 kGy holds the prospect of high effectiveness and accurate and rapid crosslinking without causing cytotoxicity of gelatin gels [147,148]. It also instantly sterilizes the media, thereby guaranteeing biomedical usage [149].…”

Section: Electron Irradiation Crosslinkingmentioning

confidence: 99%

“…Inside the class of ionizing irradiation, electron irradiation is very beneficial for the hydrogel alteration owing to its high penetration depth [150] and high dosage rates [151], which facilitates homogeneous crosslinking. Moreover, it provides accurate global as well as on-site meshing through the utilization of a highly centered electron beam, thereby paving the way for a variety of uses spanning from mechanical texturing to actuators [147,152]. Rather, for future biomedical applications including extracellular matrix models, a characterization of electron beam crosslinked collagen gels with respect to network structure, such as pore size, rheological features, and cytocompatibility, has been performed [140].…”

Section: Electron Irradiation Crosslinkingmentioning

confidence: 99%

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Bidirectional Mechanical Response Between Cells and Their Microenvironment

Mierke

2021

Front. Phys.

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Cell migration and invasion play a role in many physiological and pathological processes and are therefore subject of intensive research efforts. Despite of the intensively investigated biochemical processes associated with the migration and invasion of cells, such as cancer cells, the contribution of mechanobiological processes to the migratory capacity of cells as well as the role of physical polymeric phase transitions is not yet clearly understood. Unfortunately, these experiments are not very informative because they completely disregard the influence of the three-dimensional cell environment. Despite this data situation, it was possible to adequately demonstrate that there exists a direct mechanical interplay between cells and their microenvironment in both directions, where both elements can be mechanically altered by one another. In line with these results, it has turned out that the mechanobiological molecular processes through which cells interact with each other and additionally sense their nearby microenvironment have an impact on cellular functions such as cellular motility. The mechanotransduction processes have become the major focus of biophysical research and thereby, diverse biophysical approaches have been developed and improved to analyze the mechanical properties of individual cells and extracellular matrix environments. Both, the cell mechanics and matrix environment mechanics regulate the cell migration types in confined microenvironments and hence it seems to be suitable to identify and subsequently present a common bidirectional interplay between cells and their matrix environment. Moreover, hallmarks of the mechanophenotype of invasive cells and extracellular matrices can be defined. This review will point out how on the one hand the intracellular cytoskeletal architecture and on the other hand the matrix architecture contribute to cellular stiffness or contractility and thereby determines the migratory phenotype and subsequently the emergence of a distinct migration mode. Finally, in this review it is discussed whether universal hallmarks of the migratory phenotype can be defined.

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Section: Electron Irradiation Crosslinkingmentioning

confidence: 99%

Section: Electron Irradiation Crosslinkingmentioning

confidence: 99%

Bidirectional Mechanical Response Between Cells and Their Microenvironment

Mierke

2021

Front. Phys.

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“…This is the case for gelatin. Therefore, in order to develop substrates with mechanically patterned hydrogels, i.e., substrates with topologically controlled variations of mechanical properties, a two-step process has been proposed which combine "localized" EBL with "global" electron beam irradiation [45]. In other words, the initial stage is based on the direct effect, where the radiation energy is absorbed by the polymer molecules, and the second stage is based on the indirect effect where water absorbs the radiation energy and the aqueous radiolysis products initiate the radical-radical crosslinking.…”

Section: Patterned Hydrogels As Advanced Interfaces With Biological Smentioning

confidence: 99%

“…(c,d) Topographical and mechanical profiles of the stripe pattern after global irradiation in the wet state: (c) height and (d) E-modulus. Adapted from[45].…”

mentioning

confidence: 99%

Micro- to Nanoscale Bio-Hybrid Hydrogels Engineered by Ionizing Radiation

2020

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Bio-hybrid hydrogels consist of a water-swollen hydrophilic polymer network encapsulating or conjugating single biomolecules, or larger and more complex biological constructs like whole cells. By modulating at least one dimension of the hydrogel system at the micro- or nanoscale, the activity of the biological component can be extremely upgraded with clear advantages for the development of therapeutic or diagnostic micro- and nano-devices. Gamma or e-beam irradiation of polymers allow a good control of the chemistry at the micro-/nanoscale with minimal recourse to toxic reactants and solvents. Another potential advantage is to obtain simultaneous sterilization when the absorbed doses are within the sterilization dose range. This short review will highlight opportunities and challenges of the radiation technologies to produce bio-hybrid nanogels as delivery devices of therapeutic biomolecules to the target cells, tissues, and organs, and to create hydrogel patterns at the nano-length and micro-length scales on surfaces.

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“…On the other hand, gelatin can be crosslinked by radiation in the presence of water, i.e., in the solution state [50]. Mayer et al focused on electron beam cross-linking technology and developed gelatin and collagen hydrogels [51][52][53]. The quantum beam cross-linking technology capable of gelation without crosslinking agent is attracting attention for use in regenerative medicine and drug discovery.…”

Section: Introductionmentioning

confidence: 99%

Development of Advanced Biodevices Using Quantum Beam Microfabrication Technology

Oyama

Kimura

Nagasawa

et al. 2020

QuBS

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Biodevices with engineered micro- and nanostructures are strongly needed for advancements in medical technology such as regenerative medicine, drug discovery, diagnostic reagents, and drug delivery to secure high quality of life. The authors produced functional biocompatible plastics and hydrogels with physical and chemical properties and surface microscopic shapes that can be freely controlled in three dimensions during the production process using the superior properties of quantum beams. Nanostructures on a biocompatible poly(L-lactic acid) surface were fabricated using a focused ion beam. Soft hydrogels based on polysaccharides were micro-fabricated using a focused proton beam. Gelatin hydrogels were fabricated using γ-rays and electron beam, and their microstructures and stiffnesses were controlled for biological applications. HeLa cells proliferated three-dimensionally on the radiation-crosslinked gelatin hydrogels and, furthermore, their shapes can be controlled by the micro-fabricated surface of the hydrogel. Long-lasting hydrophilic concave structures were fabricated on the surface of silicone by radiation-induced crosslinking and oxidation. The demonstrated advanced biodevices have potential applications in three-dimensional cell culture, gene expression control, stem cell differentiation induction/suppression, cell aggregation into arbitrary shapes, tissue culture, and individual diagnosis in the medical field.

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Reagent-free mechanical patterning of gelatin surfaces by two-step electron irradiation treatment

Cited by 6 publications

References 33 publications

Bidirectional Mechanical Response Between Cells and Their Microenvironment

Bidirectional Mechanical Response Between Cells and Their Microenvironment

Micro- to Nanoscale Bio-Hybrid Hydrogels Engineered by Ionizing Radiation

Development of Advanced Biodevices Using Quantum Beam Microfabrication Technology

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