Reagent strips and spontaneous bacterial peritonitis

Castellote, José; Xiol, Xavier

doi:10.1111/j.1365-2036.2008.03749.x

Cited by 6 publications

(5 citation statements)

References 4 publications

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“…Also, Castellote and Xiol (2008) studied 228 samples of AF and diagnosed 52 episodes of SBP, using different type of strips (Autionsticksâ, A. Menarini Diagnostics, Firenze, Italy). The sensitivity and specificity of the reagent strip were 96% and 89%, respectively [10].…”

Section: Discussionmentioning

confidence: 99%

Diagnosis of spontaneous bacterial peritonitis in children using leukocyte esterase reagent strips and granulocyte elastase immunoassay

Allam

Eltaras

Hussin

et al. 2018

ceh

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Aim of the studyWe aimed to assess the utility and rapidity of granulocyte elastase (GE) latex immunoassay and leukocyte esterase (LE) reagent strips for the diagnosis of spontaneous bacterial peritonitis (SBP) in hepatic children with ascites.Material and methodsThis study included 80 ascitic fluid (AF) samples from 45 patients with chronic liver diseases. They were divided into 2 groups (SBP and non-SBP groups). White blood cells > 500 cell/mm3 or polymorphonuclear leukocytes > 250 cell/mm3 were considered as a positive result for the diagnosis of SBP. The AF obtained at bedside was immediately tested with an LE enzyme reagent strip, and another sample was aliquoted for measurement of serum GE.ResultsOn doing LE dipstick strips, all the non-SBP group gave no coloration with LE strips while 62% of the SBP group gave coloration. LE strips had accuracy of 86.25% in differentiating SBP and non-SBP at a cut-off value of 1 (color grade 1). The diagnostic performance of GE in differentiating SBP and non-SBP was assessed and showed accuracy of 70% for a cutoff value of 123.5 ng/ml.ConclusionsApplication of LE reagent strips is a bedside, rapid, easy-to-use, and inexpensive method for diagnosis of SBP. It has an accuracy of 86.25% in differentiating SBP and non-SBP, which is higher than more complicated and delayed methods such as GE latex immunoassay, which has an accuracy of 70%.

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Section: Discussionmentioning

confidence: 99%

Diagnosis of spontaneous bacterial peritonitis in children using leukocyte esterase reagent strips and granulocyte elastase immunoassay

Allam

Eltaras

Hussin

et al. 2018

ceh

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“…Random blood sugar and S. creatinine. Diagnostic paracentesis: Immediately after ascetic fluid paracentesis; the ascetic fluid was tested by the leukocyte esterase reagent strips using the Mul-tistix® dipstick (11) designed for testing urine for leukocytes. The strips were read in 120 seconds using colorimetric 5-grade scale (from 0 to 4).…”

Section: Methodsmentioning

confidence: 99%

“…Overall, the Aution® and Combur® dipsticks have performed better (regarding the negative predictive value) than the Mul-tistix®. The spectrophotometric analyzer Clinitek® 50, compatible with the Mul-tistix® dipstick, was used in only 6 studies (11) . The rather intense research on the field has brought up important details on the limitations of LERS.…”

Section: Introductionmentioning

confidence: 99%

The Diagnostic Value of Leukocyte Esterase Reagent Dip-stick in Spontaneous Bacterial Peritonitis Diagnosis in Patients with Liver Cirrhosis

Gad

El-Nemr

Saad³

2019

Suez Canal University Medical Journal

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Background: Spontaneous bacterial peritonitis (SBP) is a serious complication of advanced liver cirrhosis which requires rapid diagnosis for the initiation of proper antibiotic therapy. Manual examination of ascetic fluid is a rather time-consuming procedure. The leukocyte esterase (LE) reagent dipstick is based on the esterase activity of activated granulocytes which reacts with an ester-releasing hydroxyphenylpyrrole causing a color change in the azo dye of reagent strip. Aim: the aim of this study was to evaluate the diagnostic value of leukocyte esterase reagent dipstick in SBP diagnosis in patients with liver cirrhosis attending Suez Canal University Hospital. Methods: ascetic fluid cell count using the counting chamber method was compared to Leucocyte Esterase strip rapid test in two groups of patients (65 ABP vs 65 non-SBP). The strips were read in 120 seconds using colorimetric 5-grade scale (from 0 to 4). The grades (0-2) were considered a negative test while grades (3-4) were considered a positive one. Results: Comparison of the SBP +ve and-ve groups showed a sensitivity of LE strips of (82%) with a high specificity (94 %), PPV (93 %) and NPV (84 %) for the diagnosis of SBP. A good correlation between the LE strip color grade and the ascetic fluid PMNL cell count number (P<0.05) Conclusion: the leukocyte esterase reagent dipstick test is a rather specific, rapid, inexpensive and simple bedside method for diagnosis of SBP method for. A negative LE test result excludes SBP with a high accuracy.

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“…The utility of urinary dipstick (leukocyte esterase detection resulting from activated neutrophils) has been proposed to reach such targets, but as they were originally developed for use in urinary tract infection the cut-off values are different and the number of false negative is high. Nevertheless, none of the recent guidelines recommends the use of these reagent test strips to assess leucocyte esterase activity of activated PMNs for the diagnosis of SBP owing to unacceptable diagnostic accuracy, mostly because of the high value of false negative results [46,[49][50][51][52] . Recently, a new reagent strip test has been calibrated for ascitic fluid with a cut-off of 250 PMN/mm 3 .…”

Section: Diagnosismentioning

confidence: 99%

Spontaneous Bacterial Peritonitis: Is It Still A Life-Threatening Issue in Cirrhosis?

Ribeiro¹

2016

Journal of Gastroenterology and Hepatology Research

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Spontaneous bacterial peritonitis is the commonest and a deadly complication of cirrhosis that happens in 10% to 30% of patients admitted to hospital. The main mechanism that is being associated with its occurrence are bacterial overgrowth with translocation through the increased permeable small intestinal wall and impaired immune defense. The Gram-negative aerobic bacteria are the major pathogens responsible for SBP episodes although Gram-positive bacteria are being considered emergent agents. The ready diagnosis of SBP is a key factor for the decrease in mortality rates observed in recent years. The medical diagnosis is neither sensitive nor specific and the search for new useful and available tools to make it quicker is an important endpoint of current studies. The use of empirical antibiotics, mainly cefotaxime, improves significantly the shortterm prognosis of patients with SBP. The recurrence rate is high and antibiotic prophylaxis has been recommended in high-risk scenarios. Unfortunately, the long-term prognosis remains poor.

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Reagent strips and spontaneous bacterial peritonitis

Cited by 6 publications

References 4 publications

Diagnosis of spontaneous bacterial peritonitis in children using leukocyte esterase reagent strips and granulocyte elastase immunoassay

Diagnosis of spontaneous bacterial peritonitis in children using leukocyte esterase reagent strips and granulocyte elastase immunoassay

The Diagnostic Value of Leukocyte Esterase Reagent Dip-stick in Spontaneous Bacterial Peritonitis Diagnosis in Patients with Liver Cirrhosis

Spontaneous Bacterial Peritonitis: Is It Still A Life-Threatening Issue in Cirrhosis?

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