Reagentless biosensors based on co-entrapment of a soluble redox polymer and an enzyme within an electrochemically deposited polymer film

Vilkanauskyte, Aiste; Erichsen, Thomas; Marcinkevičienė, Liucija; Laurinavičius, Valdas; Schuhmann, Wolfgang

doi:10.1016/s0956-5663(02)00095-7

Cited by 53 publications

(26 citation statements)

References 40 publications

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“…Solvent cast films of poly(4-vinylpyridine-co-styrene) have been used as substrates for crosslinked glucose oxidase/laponite composites and have been shown to eliminate interference from acetaminophen, ureate and ascorbate [25]. Polymers such as cast films of Os-polyvinylimidazole [26] have been used in alcohol biosensors and electropolymerized Azure B films in phenol sensors [27]. An interesting variation of this method is the deposition of polydiaminobenzene from liquid crystalline solutions which have been shown to give films which were nanoporous and selectively excluded negative ions [28].…”

Section: Introductionmentioning

confidence: 99%

Polydivinylbenzene/Ethylvinylbenzene Composite Membranes for the Optimization of a Whole Blood Glucose Sensor

et al. 2006

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A novel ultra thin polydivinylbenzene/ethylvinylbenzene composite membrane has been developed for use as the outer covering barrier in a model amperometric glucose oxidase enzyme electrode. The composite membrane was formed via the cathodic electropolymerization of divinylbenzene/ethylvinylbenzene at the surface of gold sputter coated host alumina membranes, (serving solely as a mechanical support for the thin polymer film). Permeability coefficients were determined for the enzyme substrates, O 2 and glucose, across composite membranes formed with a range of polymer thicknesses. Due to the highly substrate diffusion limiting nature of the composite membrane, it was found that anionic interferents present in blood (such as ascorbate), were effectively screened from the working electrode via a charge exclusion mechanism, in a manner similar to previous findings within our laboratory. The enzyme electrode showed an initial 32% signal drift when first exposed to whole human blood over a period of 2 hours, after which time enzyme electrode responses remained essentially stable. Whole blood patient glucose determinations yielded a correlation coefficient of r 2 ¼ 0.97 in comparison to standard hospital analyses.

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Section: Introductionmentioning

confidence: 99%

Polydivinylbenzene/Ethylvinylbenzene Composite Membranes for the Optimization of a Whole Blood Glucose Sensor

et al. 2006

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“…Additionally, the complete insensitivity to oxygen tension sometimes claimed for this type of sensor has been questioned for certain mediators (Martens and Hall, 1994). A recent form of these mediator-based biosensors involves Os-containing redox hydrogels (Taylor et al, 1995;Sirkar et al, 2000;Mikeladze et al, 2002;Vilkanauskyte et al, 2002), in which the tethering of the redox couple overcomes many of the problems associated with freely-diffusing mediators. In this work, we investigate both strategies for the development of a glutamate biosensor suitable for incorporation into wells, that can be mass produced, for applications involving the release of glutamate from isolated cells.…”

Section: Introductionmentioning

confidence: 99%

Comparisons of platinum, gold, palladium and glassy carbon as electrode materials in the design of biosensors for glutamate

O’Neill

Chang

Lowry

et al. 2004

Biosensors and Bioelectronics

128

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Four electrode materials: Pt, Au, Pd and glassy carbon (GC), were studied to investigate their suitability as substrates in the development of two different classes of glutamate biosensor. Glutamate oxidase cross-linked onto poly(o-phenylenediamine) was chosen as the type 1 biosensor (PPD/GluOx), incorporating PPD as the permselective element to detect H 2 O 2 directly on the electrode surface at relatively high applied potentials. GluOx and horseradish peroxidase/redox polymer modified electrodes (Os 2+ PVP/HRP/GluOx) that relied on enzyme-catalysed H 2 O 2 detection at lower applied potentials were used as type 2 biosensors.The voltammetric and amperometric responses to the enzyme signal transduction molecule, H 2 O 2 , and the archetypal interference species in biological applications, ascorbic acid, were determined on the bare and PPD/GluOx-modified surfaces. The amperometric responses of these electrodes were stable over several days of continuous recording in phosphate buffered saline (pH 7.4). The sensitivity of the type 1 biosensors to H 2 O 2 and glutamate showed parallel trends with low limits of detection and good linearity at low concentrations: Pt > Au ∼ Pd GC. Type 2 biosensors out-performed the type 1 design for all electrode substrates, except Pt. However, the presence of the permselective PPD membrane in the type 1 biosensors, not feasible in the type 2 design, suggests that Pt/PPD/GluOx might have the best all-round characteristics for glutamate detection in biological media containing interference species such as ascorbic acid. Other points affecting a final choice of substrate should include factors such as mass production issues.

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“…An attractive approach to tackle this challenge is to use a polymeric mediator which has mediator moieties chemically attached to polymer chains. Because of its large molecular size, the polymeric mediator can be co-immobilized with enzyme at electrode by various means, including surface grafting, [31][32][33][34] layer-by-layer surface adsorption, [35][36][37] retention behind semi-permeable dialysis membranes, [38][39][40] physical entrapment [41][42][43] or cross-link in hydrogels, [44][45][46][47] entrapment in electropolymerized [48,49] or chemically formed layers [50] or in inorganic layers, [51,52] and blend in carbon pastes. [53] At LifeScan Scotland Limited, we have synthesized a ferrocene polymeric mediator which is a copolymer of vinylferrocene, acrylamide and 2-(diethylamino)ethyl methacrylate.…”

Section: Fad-gdh Biosensor For Continuous Glucose Monitoringmentioning

confidence: 99%

Amperometric Glucose Sensors for Whole Blood Measurement Based on Dehydrogenase Enzymes

Cardosi¹,

Liu²

2012

Dehydrogenases

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Reagentless biosensors based on co-entrapment of a soluble redox polymer and an enzyme within an electrochemically deposited polymer film

Cited by 53 publications

References 40 publications

Polydivinylbenzene/Ethylvinylbenzene Composite Membranes for the Optimization of a Whole Blood Glucose Sensor

Polydivinylbenzene/Ethylvinylbenzene Composite Membranes for the Optimization of a Whole Blood Glucose Sensor

Comparisons of platinum, gold, palladium and glassy carbon as electrode materials in the design of biosensors for glutamate

Amperometric Glucose Sensors for Whole Blood Measurement Based on Dehydrogenase Enzymes

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