Reagentless Immunosensing Assay via Electrochemical Impedance for Hepatitis B Surface Antigen Monitoring Based on Polypyrrole and Gold Nanoparticles as Matrices

Abstract

A novel simple immunosensing strategy for fabrication of hepatitis B surface antigen detection has been developed via electrochemical impedance spectroscopy (EIS) as a platform. At first, the conductive polymer polypyrrole (PPy) film was electrodeposited on a platinum electrode surface to adsorb the gold nanoparticles (nano-Au) via the opposite-charged adsorption technique, and then hepatitis B surface antibodies were adsorbed onto the surface of nano-Au. The modification procedure was characterized by EIS. Such spectroscopy is attributed to the concomitant conductivity changes of the polymerized pyrrole film and gold nanoparticles. The factors influencing the performance of resulting immunoelectrode were studied in detail. The linear range of the resulting immunoelectrode is from 2.6 to 153.6 ng•mL -1 with a detection limit of 1.3 ng•mL -1 at 3σ. In addition, the experiment results indicate that antibody immobilized on this way exhibits a good sensitivity, selectivity, high stability and a long-term maintenance of bioactivity, implying a great promising alternative approach for reagentless immunosensing analysis in the clinical diagnosis.

Role of metal and metal oxide nanoparticles as diagnostic and therapeutic tools for highly prevalent viral infections

“…Gold nanoparticles attached to magnetic micro-particles and functionalized with multiple restriction enzyme targets (BamHI for HAV target, SpeI for HBV target, and EcoRV for HIV target) have been used for femto molar detection of hepatitis viral DNA [57]. Multiple studies based on antibody coated gold nanoparticles for potentiometric [58], amperometric [59], impedance [60], and electrochemical [61–63] immuno-sensors have also been developed for the detection hepatitis antigens in human serum.…”

Applicability of Metal Nanoparticles in the Detection and Monitoring of Hepatitis B Virus Infection

“…Chen et al designed and tested an impedance biosensor for HBV DNA which had a LOD of 111 copies/mL [ 35 ]. Several electrochemical biosensors have been developed to detect HBsAg [ 36 , 37 , 38 , 39 , 40 , 41 , 42 ], with sensitivities ranging from 0.358 pg/mL [ 36 ] to 1.9 pg/mL.…”

Piezoelectric immunosensor with gold nanoparticles enhanced competitive immunoreaction technique for quantification of aflatoxin B1

“…Immunosensors have become increasingly practical and useful tools in biotechnology (Lu et al, 2000), clinical diagnostics (Fu et al, 2006), environmental monitoring (Steegborn and Skládal, 1997), and food industry (Sapsford et al, 2006a,b;Van der Gaag et al, 2003). Of the various kinds of immunosensors, the piezoelectric quartz crystal (PQC) immunosensor has been extensively applied to biorecognition sensing due to the advantages of cost-effectiveness, direct detection, experimental simplicity, and real-time output (Yao, 1997;Bunde et al, 1998;Janshoff et al, 2000).…”