Reaktionen von 1,5‐difluor‐2,4‐dinitrobenzol mit insulin 2. Mitt. Versuche mit insulin

Zahn, Von Helmut; Meienhofer, Johannes

doi:10.1002/macp.1958.020260113

Cited by 61 publications

(14 citation statements)

References 28 publications

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“…The intramolecular reaction resulted in the formation of a cross-link between the amino groups of A1-glycine and B29-lysine far apart from each other. Our conclusion [19] was, that in the tertiary structure…”

Section: Cross-linking the Amino Groups Of A1-glycine And B29-lysine mentioning

confidence: 82%

“…In those days the covalent structure of the A and B chains [19,20] was known, but neither the position of the disulfide bonds nor the molecular weight had been established unambiguously. Meienhofer synthesized all the amino acid derivatives of FFDNB and FFsulfone which were expected from reactions with insulin and were not yet available.…”

Section: Cross-linking the Amino Groups Of A1-glycine And B29-lysine mentioning

confidence: 99%

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My journey from wool research to insulin

Zahn¹

2000

J. Peptide Sci.

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Section: Cross-linking the Amino Groups Of A1-glycine And B29-lysine mentioning

confidence: 82%

Section: Cross-linking the Amino Groups Of A1-glycine And B29-lysine mentioning

confidence: 99%

My journey from wool research to insulin

Zahn¹

2000

J. Peptide Sci.

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“…Unlike mono‐functional probes that can provide the position of solvent‐protected residues, but fail to identify the structure responsible for protection, cross‐linking agents are capable of recognizing pairwise residues that were located within the probe span. This characteristic enables the direct evaluation of interresidue distances in proteins56–58 and nucleic acids,59, 60 which is at the basis of their application to structure determination. At the same time, the possibility of latching onto functional groups that are juxtaposed only temporarily by transient conformations makes them ill suited for the investigation of dynamics.…”

Section: The Evolving Trajectory Of Chemical Methods In Structural Bimentioning

confidence: 99%

Elucidating the higher‐order structure of biopolymers by structural probing and mass spectrometry: MS3D

Fabris

2010

J. Mass Spectrom.

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Chemical probing represents a very versatile alternative for studying the structure and dynamics of substrates that are intractable by established high-resolution techniques. The implementation of MS-based strategies for the characterization of probing products has not only extended the range of applicability to virtually all types of biopolymers, but has also paved the way for the introduction of new reagents that would not have been viable with traditional analytical platforms. As the availability of probing data is steadily increasing on the wings of the development of dedicated interpretation aids, powerful computational approaches have been explored to enable the effective utilization of such information to generate valid molecular models. This combination of factors has contributed to making the possibility of obtaining actual 3D structures by MS-based technologies (MS3D) a reality. Although approaches for achieving structure determination of unknown substrates or assessing the dynamics of known structures may share similar reagents and development trajectories, they clearly involve distinctive experimental strategies, analytical concerns, and interpretation paradigms. This Perspective offers a commentary on methods aimed at obtaining distance constraints for the modeling of full-fledged structures, while highlighting common elements, salient distinctions, and complementary capabilities exhibited by methods employed in dynamics studies. We discuss critical factors to be addressed for completing effective structural determinations and expose possible pitfalls of chemical methods. We survey programs developed for facilitating the interpretation of experimental data and discuss possible computational strategies for translating sparse spatial constraints into all-atom models. Examples are provided to illustrate how the concerted application of very diverse probing techniques can lead to the solution of actual biological substrates.

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“…131 All the complexes described gave correct elemental analyses. [4] We are grateful to D. K . Ohigashi for recording and discussing the ESR spectra.…”

Section: Diallyidihydropentulenylenedinickel (2)mentioning

confidence: 98%

Allyldihydropentalenylene Complexes of Transition Metals

Miyake

Kanai

1971

Angew. Chem. Int. Ed. Engl.

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that the modification of only one j 3 chain is not enough to block the interaction of the molecule as a whole with oxygen. Since this derivative also exhibits the myoglobin type of oxygen-binding curve, the interchain crosslinking, i. e. the fixation of the quaternary structure of the protein according to the model, can hinder the rearrangement from the deoxy form into the oxy form as effectively as the blockage of the terminal histidines of the p chains. The change in the quaternary structure, according to these experiments, is then a necessary condition for the transition between the oxy and deoxy forms of the tertiary structures.

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Reaktionen von 1,5‐difluor‐2,4‐dinitrobenzol mit insulin 2. Mitt. Versuche mit insulin

Cited by 61 publications

References 28 publications

My journey from wool research to insulin

My journey from wool research to insulin

Elucidating the higher‐order structure of biopolymers by structural probing and mass spectrometry: MS3D

Allyldihydropentalenylene Complexes of Transition Metals

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