2020
DOI: 10.3390/v12010116
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Real Time Analysis of Bovine Viral Diarrhea Virus (BVDV) Infection and Its Dependence on Bovine CD46

Abstract: Virus attachment and entry is a complex interplay of viral and cellular interaction partners. Employing bovine viral diarrhea virus (BVDV) encoding an mCherry-E2 fusion protein (BVDVE2-mCherry), being the first genetically labelled member of the family Flaviviridae applicable for the analysis of virus particles, the early events of infection—attachment, particle surface transport, and endocytosis—were monitored to better understand the mechanisms underlying virus entry and their dependence on the virus recepto… Show more

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Cited by 11 publications
(8 citation statements)
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“…The cellular uptake of BVDV particles is mediated by Clathrin-dependent endocytosis and it was shown to be dependent on interaction between viral E2 and cellular CD46 bov (14,16,33). For BVDV, CD46 bov was identified to function as the main receptor, but there are several lines of evidence indicating that an additional receptor may exist (23,33,37,38).…”
Section: Discussionmentioning
confidence: 99%
“…The cellular uptake of BVDV particles is mediated by Clathrin-dependent endocytosis and it was shown to be dependent on interaction between viral E2 and cellular CD46 bov (14,16,33). For BVDV, CD46 bov was identified to function as the main receptor, but there are several lines of evidence indicating that an additional receptor may exist (23,33,37,38).…”
Section: Discussionmentioning
confidence: 99%
“…It mediates virus attachment by binding to the cellular receptor, membrane cofactor protein (CD46) which is the only known receptor identified so far for BVDV [ 18 ]. However, the available evidence suggests that CD46 is an attachment receptor that is not sufficient for virus entry [ 19 , 20 ]. Therefore, interaction with other proteins that act as co-receptors or alternative receptors may be necessary for virus entry [ 19 , 20 ].…”
Section: Introductionmentioning
confidence: 99%
“…However, the available evidence suggests that CD46 is an attachment receptor that is not sufficient for virus entry [ 19 , 20 ]. Therefore, interaction with other proteins that act as co-receptors or alternative receptors may be necessary for virus entry [ 19 , 20 ]. The ncp BVDV can be transmitted in a wide range of body fluids, including nasal discharge, saliva, tears, urine, milk, semen, and fetal fluids [ 6 , 21 , 22 ].…”
Section: Introductionmentioning
confidence: 99%
“…A first and fundamental step in a viral infection, is attachment to a cellular membrane molecule, a viral receptor. In this issue, two articles focus on the purported receptor for BVDV, bovine CD46 [ 12 , 13 ], describing new tools for the study of the interaction between BVDV and CD46 and suggesting, perhaps not surprisingly considering what we know about receptors for other viruses [ 14 ], that CD46 may be but one of two or more molecules on the target cell surface membrane necessary for viral uptake [ 12 ].…”
mentioning
confidence: 99%
“…There remains many questions to be addressed even in those very same areas dealt with in this issue, including the conditions, at both the cellular and organismic level, that are conducive to events leading to biotype-switch, viral persistence, emergence of new pestivirus diseases as well as some of the discrepancies between in vitro and in vivo results in regard to immune responses—to mention just a few. Development of new approaches to the investigation of “old” and new pestiviruses are already under way [ 12 , 13 , 29 ] and others are likely to become available in the future.…”
mentioning
confidence: 99%