Real-time and label-free detection of <i>VKORC1</i> genes based on a magnetoelastic biosensor for warfarin therapy

Sang, Shengbo; Guo, Xing; Wang, Jingzhe; Li, Hongmei; Ma, Xianyong

doi:10.1039/d0tb00354a

Cited by 11 publications

(6 citation statements)

References 36 publications

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“…In a similar way, Sang et al expanded the use of ME sensors to detect warfarin doses by monitoring VKORC1 genotypes. 89 The biocompatibility of the ME sensor was improved using gold layers, while the functionalization with thiolated capture probes, tDNA, and biotin layers leads to a high sensitivity and specific detection. The fabricated sensors show fast and linear responses in the range of 0.1 fM to 10 pM, which demonstrates the possibility of employing cheap magnetoelastic sensors for the biomedical detection of warfarin doses, among others.…”

Section: ■ Application Fields Of Magnetoelastic Resonance Devicesmentioning

confidence: 99%

Magnetoelastic Resonance Sensors: Principles, Applications, and Perspectives

et al. 2022

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Magnetoelastic resonators are gaining attention as an incredibly versatile and sensitive transduction platform for the detection of varied physical, chemical, and biological parameters. These sensors, based on the coupling effect between mechanical and magnetic properties of ME platforms, stand out in comparison to alternative technologies due to their low cost and wireless detection capability. Several parameters have been optimized over the years to improve their performance, such as their composition, surface functionalization, or shape geometry. In this review, the working principles, recent advances, and future perspectives of magnetoelastic resonance transducers are introduced, highlighting their potentials as a versatile platform for sensing applications. First, the fundamental principles governing the magnetoelastic resonators performance are introduced as well as the most common magnetoelastic materials and their main fabrication methods are described. Second, the versatility and technical feasibility of magnetoelastic resonators for biological, chemical, and physical sensing are highlighted and the most recent results and functionalization processes are summarized. Finally, the forefront advances to further improve the performance of magnetoelastic resonators for sensing applications have been identified.

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Section: ■ Application Fields Of Magnetoelastic Resonance Devicesmentioning

confidence: 99%

Magnetoelastic Resonance Sensors: Principles, Applications, and Perspectives

et al. 2022

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“…The strong binding between biotin and avidin has high affinity and multistage amplification, [ 57,58 ] which has been widely used in the qualitative and quantitative detection of trace antigens and antibodies, even localization observation research. In order to guide rational clinical therapy with warfarin, Sang et al [ 24 ] introduced BAS to amplify the signal and improve sensitivity, and they also demonstrated for the first time an ME ‐based biosensor to wirelessly detect real‐time and label‐free vitamin K epoxide reductase complex subunit 1 (VKORC1) genes. In Figure 4c, the thiolated capture probes (CPs) were covalently immobilized on the surface of the gold‐plated Metglas alloy 2826 surface as the first specific recognition layer for detection of target DNA (tDNA) molecules.…”

Section: Surface Functionalizationmentioning

confidence: 99%

“…[6][7][8][9] Figure 1 shows an overview of the main approaches to using transducers in ME biosensors and application prospects in related fields. For instance, by using various kinds of surface functionalization and bioanalysis, ME biosensors are used to detect classical swine fever virus in wild animals, [10,11] monitor the release of pesticides, [12][13][14] and metal ions [15,16] into the environment, [17][18][19] and detect various diseases analyses (such as, diabetes, [20][21][22] genetic diseases [23][24][25] and cancers [26,27] ). Hence, the ME biosensor has a promising prospect in clinical use and environmental protection.…”

Section: Introductionmentioning

confidence: 99%

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Surface Functionalization, Bioanalysis, and Applications: Progress of New Magnetoelastic Biosensors

Yuan

Huang

et al. 2022

Adv Eng Mater

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Magnetoelastic (ME) biosensors are portable, nontoxic, inexpensive, compatible with wireless networks, and have high sensitivity, accuracy, and specificity toward the target element. Thus, they have attracted considerable attention in application of biological identification, chemical detection, and medical diagnosis, as well as in many academic achievements in the field of sensor research. Herein, the recent progress of ME biosensors, including surface functionalization, bioanalysis, and its applications is critically reviewed. The remaining scientific and technological challenges related to the development of novel ME biosensors with new materials and structures in this field are elaborated, too. This review not only can provide a guideline for researchers in future development of ME biosensors, but also further improves their detection performance and expand the scope of their applications.

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“…The major drawback of this detection method is its limitation on the size as it typically works for high molecular weight analytes since it reduces the oscillation frequency. Various types of anisotropic materials help in sensing, for example, aluminum nitride, zinc oxide, barium titanate, lead titanate, quartz, and poly (vinylidene fluoride) [129,130]. The first piezoelectric immunosensor was able to detect the coronavirus in sputum.…”

Section: Piezoelectric Biosensormentioning

confidence: 99%

COVID-19 Biomarkers and Advanced Sensing Technologies for Point-of-Care (POC) Diagnosis

et al. 2021

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COVID-19, also known as SARS-CoV-2 is a novel, respiratory virus currently plaguing humanity. Genetically, at its core, it is a single-strand positive-sense RNA virus. It is a beta-type Coronavirus and is distinct in its structure and binding mechanism compared to other types of coronaviruses. Testing for the virus remains a challenge due to the small market available for at-home detection. Currently, there are three main types of tests for biomarker detection: viral, antigen and antibody. Reverse Transcription-Polymerase Chain Reaction (RT-PCR) remains the gold standard for viral testing. However, the lack of quantitative detection and turnaround time for results are drawbacks. This manuscript focuses on recent advances in COVID-19 detection that have lower limits of detection and faster response times than RT-PCR testing. The advancements in sensing platforms have amplified the detection levels and provided real-time results for SARS-CoV-2 spike protein detection with limits as low as 1 fg/mL in the Graphene Field Effect Transistor (FET) sensor. Additionally, using multiple biomarkers, detection levels can achieve a specificity and sensitivity level comparable to that of PCR testing. Proper biomarker selection coupled with nano sensing detection platforms are key in the widespread use of Point of Care (POC) diagnosis in COVID-19 detection.

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Real-time and label-free detection of VKORC1 genes based on a magnetoelastic biosensor for warfarin therapy

Abstract: This work describes the design of a magnetoelastic biosensor to detect VKORC1 genes for warfarin therapy in a fast, label-free and sensitive manner.

Cited by 11 publications

References 36 publications

Magnetoelastic Resonance Sensors: Principles, Applications, and Perspectives

Magnetoelastic Resonance Sensors: Principles, Applications, and Perspectives

Surface Functionalization, Bioanalysis, and Applications: Progress of New Magnetoelastic Biosensors

COVID-19 Biomarkers and Advanced Sensing Technologies for Point-of-Care (POC) Diagnosis

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