Real‐time atomic force microscopy of root dentine during demineralization when subjected to chelating agents

De‐Deus, Gustavo; Paciornik, Sidnei; Maurício, M. H. P.; Prioli, R.

doi:10.1111/j.1365-2591.2006.01128.x

Cited by 55 publications

(56 citation statements)

References 39 publications

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“…This point represents a progress from the traditional qualitative SEM studies for the characterization of the dentin surface (22)(23)(24). There appear to be few reports in the literature involving longitudinal and quantitative analysis of the process of dentin demineralization.…”

Section: Discussionmentioning

confidence: 99%

Longitudinal Co-site Optical Microscopy Study on the Chelating Ability of Etidronate and EDTA Using a Comparative Single-tooth Model

De‐Deus¹,

Zehnder²,

Reis³

et al. 2008

Journal of Endodontics

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Longitudinal co-site optical microscopy study on the chelating ability of etidronate and EDTA using a comparative single-tooth model AbstractIn the present study the smear layer dissolution kinetics of 18% etidronate (HEBP), 9% HEBP, and 17% ethylenediaminetetraacetic acid (EDTA) on human dentin were quantitatively and longitudinally analyzed by using a single-tooth comparative model. Coronal dentin disks were prepared from 3 maxillary human molars. A standardized smear layer was produced on the pulpal side of each disk. The smear layer-covered surface was divided into 3 similar areas. Each of these was then exposed to 1 of the 3 irrigants under investigation, whereas the others were covered with adhesive tape. Co-site image sequences of the areas under investigation were obtained after several cumulative demineralization times. Sixteen images were obtained from each dentin area of each tooth for each experimental time at 1000x magnification. An image processing and analysis sequence measured sets of images, providing data of area fraction for thousands of tubules over time and allowing us to quantitatively follow the effect of the chelating substances. The Kruskal-Wallis H test and Dunn multiple comparison test were used to analyze the data. Overall, it can be concluded that the demineralization kinetics promoted by both 9% HEBP and 18% HEBP were significantly slower than those of 17% EDTA (P < .05). In addition, the single-tooth model is advantageous over the first co-site optical microscopy dentin assessments when different chelator solutions are compared. Longitudinal Co-site Optical Microscopy Study of the Chelating Ability of HEBP and EDTA Using a Comparative Single-tooth Model AbstractIn the present study the smear layer dissolution kinetics of 18% HEBP, 9% HEBP and 17%EDTA on human dentin were quantitatively and longitudinally analyzed using a singletooth comparative model. Coronal dentin disks were prepared from three maxillary human molars. A standardized smear layer was produced on the pulpal side of each disk. The smear layer-covered surface was divided into three similar areas. Each of these was then exposed to one of the three irrigants under investigation, while the others were covered with adhesive tape. Co-site image sequences of the areas under investigation were obtained after several cumulative demineralization times. Sixteen images were obtained from each dentin area of each tooth for each experimental time, at 1000x magnification. An image processing and analysis sequence measured sets of images, providing data of area fraction for thousands of tubules over time, allowing to quantitatively follow the effect of the chelating substances. The Kruskal-Wallis H-test and Dunn's multiple comparison test were used to analyze the data. Overall, it can be concluded that the demineralization kinetics promoted by both 9% HEBP and 18% HEBP were significantly slower than those of 17% EDTA (p < 0.05). In addition, the single-tooth model is advantageous over the first co-site optical microscopy dentin asse...

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Section: Discussionmentioning

confidence: 99%

Longitudinal Co-site Optical Microscopy Study on the Chelating Ability of Etidronate and EDTA Using a Comparative Single-tooth Model

De‐Deus¹,

Zehnder²,

Reis³

et al. 2008

Journal of Endodontics

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“…EDTA: 17% EDTA at the apical third, 2,000Â; citric: 42% citric acid at the apical third, 2,000Â; MTAD: MTAD at the apical third, 2,000Â; and control: control group at the apical third, 2,000Â. apical third although erosive effects of EDTA and citric acid have been reported in several studies (12,31,(39)(40)(41). BioPure MTAD is comparatively more aggressive in demineralizing intact intraradicular dentin and was able to expose collagen matrices 1.5 to 2 times as thick as those produced with EDTA (42).…”

Section: Discussionmentioning

confidence: 99%

A Comparative Study of Smear Layer Removal and Erosion in Apical Intraradicular Dentine With Three Irrigating Solutions: A Scanning Electron Microscopy Evaluation

Mancini

Armellin

Casaglia

et al. 2009

Journal of Endodontics

111

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Introduction: This study compared the efficacy of BioPure MTAD (Dentsply Tulsa, Tulsa, OK), 17% EDTA, and 42% citric acid in endodontic smear layer removal and degree of erosion in the apical third of endodontic canals. Methods: Ninety-six extracted single-rooted human teeth were randomized into four groups (n = 24) and instrumented using System GT nickel-titanium rotary instruments (Dentsply Tulsa, Tulsa, OK). Each canal was irrigated with one of the following solutions: BioPure MTAD, 17% EDTA, 42% citric acid, or 5.25% NaOCl (control). Next, all specimens were irrigated with 5.25% NaOCl. Results: Evaluation by scanning electron microscopy showed no significant differences among test irrigants in removing the smear layer. However, the efficacy of BioPure MTAD and 17% EDTA in removing the smear layer was significantly greater than 5.25% NaOCl (control). The erosive effects of irrigating solutions could not be evaluated. Conclusions: In conclusion, the protocols used in this study were not sufficient to completely remove the smear layer in the apical third of prepared root canals. (J Endod 2009;35:900-903)

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“…A metallographic preparation was performed only on the coronallyfacing surface of each slice [19]. Then, these surfaces were examined under CLSM (Carl Zeiss, Jena, Germany) and images were recorded using the fluorescent mode (laser wavelengths of 568 nm) with the maximum resolution of about 220 nm laterally and 330 nm axially.…”

Section: Confocal Laser Scanning Microscopymentioning

confidence: 99%

Resin Tags Have No Contribution On Push-Out Bond Strength Of Self-Adhesive Resin Cement

Crivano¹

2014

Dentistry

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Aim: To assess the contribution of resin tags in the interfacial bond strength of self-adhesive cement used to lute fiber posts into root canals.Methodology: Twenty extracted human canine teeth were selected and root filled. Roots were randomly assigned into two groups (n=10) according to pre-treatment of root dentin: non-etched (G1) or etched using 37% phosphoric acid (G2). Glass fiber posts (White Post) were luted into the root canals using the self-adhesive cement RelyX TM UniChem. Before insertion of the post, the cement was labeled with Rhodamine B isothiocyanate. Roots were sectioned transversally into slices of 1.5 mm thickness to perform the tests at the coronal, middle and apical regions of the root canal. Each slice was analyzed using confocal laser scanning microscopy to determine the presence or absence of resin tags, and then, subjected to a push-out test. Bond strengths data (MPa) were analyzed by Student t-test (α=0.05). Results:The qualitative analysis of the post-dentin interface revealed that was not possible to observe the formation of resin tags into the root dentin in G1, whereas all slices belonging to G2 exhibited the presence of resin tags. For the push-out test, there were no significant differences between G1 (10.5 ± 3.53) and G2 (10.61 ± 3.84) groups (p<0.05) in all root canal regions. Conclusions:Resin tags, as a result of root dentin etching, did not affect the push-out bond strength of fiber posts to root canals when using the self-adhesive cement RelyX TM Uni Cem.

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Real‐time atomic force microscopy of root dentine during demineralization when subjected to chelating agents

Abstract: The most effective demineralizing substance was citric acid. The methodology developed for real-time observation of dentine surfaces is a valuable method to evaluate demineralization.

Cited by 55 publications

References 39 publications

Longitudinal Co-site Optical Microscopy Study on the Chelating Ability of Etidronate and EDTA Using a Comparative Single-tooth Model

Longitudinal Co-site Optical Microscopy Study on the Chelating Ability of Etidronate and EDTA Using a Comparative Single-tooth Model

A Comparative Study of Smear Layer Removal and Erosion in Apical Intraradicular Dentine With Three Irrigating Solutions: A Scanning Electron Microscopy Evaluation

Resin Tags Have No Contribution On Push-Out Bond Strength Of Self-Adhesive Resin Cement

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