Real time biosensor analysis of Staphylococcal enterotoxin A in food

Rasooly, Linda; Rasooly, Avraham

doi:10.1016/s0168-1605(99)00053-7

Cited by 66 publications

(34 citation statements)

References 28 publications

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“…Abdel-Hamid et al [37] developed a sensor for E. coli O157 : H7 with a limit of detection (LOD) of 100 cells/mL. Rasooly and Rasooly [38] developed a biosensor for staphylococcal enterotoxin A in food. Their system is rapid, responding in less than 4 min using an evanescent wave detector in a capture format.…”

Section: Immunosensors For Pathogenic Bacteria or Pesticides In Foodmentioning

confidence: 99%

Sensors and Food Quality

Connell

Guilbault

2001

Sensors Update

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Production processes require quality control. The quality of food going to the consumer must always be assured. Traditionally this was performed by taking a sample and sending it to a remote laboratory. While this is successful for most samples, this was not always the case. Having remote laboratories means that there could be a significant lag-time between sampling and results. Some unstable samples may have changed or the source material could have been already used/processed by the time they are analyzed. Sensor-based systems, especially those incorporated within flow injection analyzers, can overcome this problem. As the systems can be small and inexpensive relative to other automated analyzers, they can easily be fitted onsite. With the sensors fitted within the production process, a nearly instant reading can be achieved. The advantages and disadvantages of physical, chemical, and biosensors and their applicability to food analysis are discussed. Particular attention is focused on the improvements made to the underlying technology used in biosensor construction.

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Section: Immunosensors For Pathogenic Bacteria or Pesticides In Foodmentioning

confidence: 99%

Sensors and Food Quality

Connell

Guilbault

2001

Sensors Update

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“…The suspension was centrifuged for 10 min and the supernatant was used for analysis. [10] All samples were analyzed by the flow injection capacitive immunosensor under its optimum conditions.…”

Section: Determination Of Sea In Real Samplesmentioning

confidence: 99%

“…For the indirect format, studies have been based on the detection of labeling agents in a sandwich or competitive assay. The use of a quartz crystal microbalance, [9] surface plasmon resonance, [10,11] fiberoptics [12] and conductometry [13] have also been reported. Indirect detection methods can provide a limit of detection down to µg L −1 .…”

Section: Introductionmentioning

confidence: 99%

Detection of staphylococcal enterotoxin A (SEA) at picogram level by a capacitive immunosensor

Jantra¹,

Kanatharana²,

Asawatreratanakul³

et al. 2011

Journal of Environmental Science and Health, Part A

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This work presents the use of a flow injection capacitive immunosensor to detect staphylococcal enterotoxin A (SEA). The study was based on the direct detection of a capacitance change due to the binding between SEA and anti-SEA immobilized on a gold electrode. The optimal regeneration solution, flow rate, sample volume and buffer conditions were studied. Under the optimum conditions, this label-free biosensor provided linearity between 1 × 10(-12) g L(-1) and 1 × 10(-8) g L(-1) of SEA and the limit of detection was 1 × 10(-12) g L(-1) which was much lower than the infectious dose (0.5 × 10(-6) - 1 × 10(-6) g L(-1)). Using the regeneration solution of, 15.0 mM glycine-HCl pH 2.20, to break the binding between SEA and the immobilized anti-SEA enabled the electrode to be reused up to 39 times. This technique was applied to analyze SEA in liquid and solid food samples. Any matrix effect can be eliminated by simple dilution. SEA contamination was found in three samples, iced tea with milk (28 ± 1 ng L(-1)), orange juice (113 ± 6 ng L(-1)) and fried chicken (1.1 ± 0.2 ng g(-1)); however, the concentrations were much lower than the infectious dose. The proposed method would be useful for rapid screening of SEA in various matrices.

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“…A sandwich-type assay can be carried out within 4 min measuring the change of the refractive index of the sensor surface. The sensitivity is in the range of 10-100 ng/g depending on the type of food sample [118]. An overview of sensor formats for bacterial toxins and spores is shown in Table 3.…”

Section: Botulinum Toxinsmentioning

confidence: 99%

Toxin immunosensors and sensor arrays for food quality control

Moises

Schäferling

2009

Bioanal Rev

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The global efforts to improve consumer protection and public health lead to an increasing number of analytical approaches applicable to food analysis and process control. Biosensor systems are efficient analytical tools to monitor production processes or storage of nutrition and to control contamination outbreaks as they are easy-touse, fast, and with minimal effort on sample preparation. Relevant targets of immunosensors implemented to food safety are prevalent bacterial toxins (staphylococcal enterotoxins and clostridial toxins), plant toxins (Ricin), mycotoxins (aflatoxins and ochratoxin A), marine toxins, and other pathogenic bacterial contaminations (Listeria, Salmonella, Staphylococcus aureus, or Escherichia coli). These cause acute intoxication and also chronic diseases in humans consuming contaminated food. Promising approaches for the determination of different types of toxins in food matrices will be outlined. The corresponding sensor systems use immunological receptor units such as antibodies or antigens and include optical (fluorescence and surface plasmon resonance), electrochemical, or acoustical readout methods. This review is focused on recent developments of sensor formats devoted to food safety control and is structured according to the type of toxin or contaminant that is recognized. It is intended to give an overview on emerging sensor technologies and their potential applications for the rapid analysis of the most important food poisoning agents.

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Real time biosensor analysis of Staphylococcal enterotoxin A in food

Cited by 66 publications

References 28 publications

Sensors and Food Quality

Sensors and Food Quality

Detection of staphylococcal enterotoxin A (SEA) at picogram level by a capacitive immunosensor

Toxin immunosensors and sensor arrays for food quality control

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