Real-time, continuous, fluorescence sensing in a freely-moving subject with an implanted hybrid VCSEL/CMOS biosensor

O’Sullivan, Thomas D.; Heitz, Roxana T.; Parashurama, Natesh; Barkin, David B.; Wooley, B.A.; Gambhir, Sanjiv S.; Harris, James S.; Levi, Ofer

doi:10.1364/boe.4.001332

Cited by 15 publications

(15 citation statements)

References 17 publications

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“…O’Sullivan et al (O’Sullivan et al 2013) reported the fabrication of a miniature, integrated semiconductor-based fluorescence sensor, designed for detecting fluorophores and tumor-targeted molecular probes (Fig. 2f).…”

Section: Miniaturized Optical Systems Based On Exogenous Contrast Agentsmentioning

confidence: 99%

“…When the system was tested on freely moving mice, the authors reported an increase of ~ 500 pA in the photocurrent following injection of Cy 5.5 fluorescence dye. This miniaturized system required precise positioning and fixation to the targeted object, which made it difficult to use on soft tissue or when imaging small targets (O’Sullivan et al 2013). …”

Section: Miniaturized Optical Systems Based On Exogenous Contrast Agentsmentioning

confidence: 99%

“…The image is the standard deviation computed from a ten second acquisition, to emphasize the vasculature. (Scale bar = 50 μm) (f) Photograph of the VCSEL-CMOS sensor reported by (O’Sullivan et al 2013). The custom PCB with bonded chips, consists of multiple VCSEL excitation sources, two GaAs photodiodes and a CMOS read-out integrated circuit (ROIC).…”

Section: Figurementioning

confidence: 99%

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Miniaturized optical neuroimaging in unrestrained animals

Senarathna

Tyler

et al. 2015

NeuroImage

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The confluence of technological advances in optics, miniaturized electronic components and the availability of ever increasing and affordable computational power have ushered in a new era in functional neuroimaging. Namely, an era in which neuroimaging of cortical function in unrestrained and unanesthetized rodents has become a reality. Traditional optical neuroimaging required animals to be anesthetized and restrained. This greatly limited the kinds of experiments that could be performed in vivo. Now one can assess blood flow and oxygenation changes resulting from functional activity, image functional response in disease models such as stroke and seizure, and even conduct long-term imaging of tumor physiology, all without the confounding effects of anesthetics or animal restraints. These advances are shedding new light on mammalian brain organization and function, and helping to elucidate loss of this organization or ‘dysfunction’ in a wide array of central nervous system disease models. In this review, we highlight recent advances in the fabrication, characterization and application of miniaturized head-mounted optical neuroimaging systems pioneered by innovative investigators from a wide array of disciplines. We broadly classify these systems into those based on exogenous contrast agents, such as single- and two-photon microscopy systems; and those based on endogenous contrast mechanisms, such as multispectral or laser speckle contrast imaging systems. Finally, we conclude with a discussion of the strengths and weaknesses of these approaches along with a perspective on the future of this exciting new frontier in neuroimaging.

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Section: Miniaturized Optical Systems Based On Exogenous Contrast Agentsmentioning

confidence: 99%

Section: Miniaturized Optical Systems Based On Exogenous Contrast Agentsmentioning

confidence: 99%

Section: Figurementioning

confidence: 99%

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Miniaturized optical neuroimaging in unrestrained animals

Senarathna

Tyler

et al. 2015

NeuroImage

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“…Usually, molecular diagnostic is a costly procedure. A research report previously demonstrated the capability of CMOS sensor-based molecular diagnostics [28,35,94]. Indeed, CMOS sensors constitute a suitable device for POC applications due to their availability in mobile phone cameras and user-friendliness.…”

Section: Future Perspectivesmentioning

confidence: 99%

Overview of CMOS image sensor use in molecular diagnostics

Devadhasan

Yoo

Kim

2015

Current Applied Physics

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“…In particular, narrowband photodetection in the far‐red range (i.e., photodetectors with peak response at 700–750 nm)—dimly visible to the human eye and at the same time distinct from the infrared region—is of considerable interest, in view of its role in many natural processes and technological applications. Narrowband photodetection in the far‐red range is relevant to analytical biology (e.g., flow cytometry32), medical diagnostics (e.g., brain imaging,33 in‐vivo tumor detection34), remote sensing,35 botany,36 precision agriculture,37 and food quality inspection 38. It is important to note that a large number of such applications require spectral widths loosely around 100 nm, in principle within reach of the NBA approach—while ultranarrowband passbands (spectral with ≲50 nm) would lead to poor photon collection, hence would be unsuitable for the purpose.…”

Section: Introductionmentioning

confidence: 99%

Narrowband‐Absorption‐Type Organic Photodetectors for the Far‐Red Range Based on Fullerene‐Free Bulk Heterojunctions

Xia

Wang

et al. 2020

Advanced Optical Materials

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chemical tailoring, [12] their facile lowtemperature processing, [13] and their mechanical flexibility, [14] organic semiconductors bear the promise of delivering narrowband photodetection with high performance and in unconventional settings and form factors, [15] e.g., in low-cost, point-of-use devices for wearable optoelectronics, [16,17] the Internet of Things, [18] computer vision, [19] and biomedicine. [20] Among the several organic narrowband photodetection strategies that have been investigated to date, the so-called narrowband absorption (NBA) strategy-relying on a photoactive layer predominantly absorbing in the target spectral range-has been prominent. [11,15] Alternative narrowband strategies relying on internal optoelectronic filtering effects (such as charge carrier narrowing [21] and charge-injection narrowing [5,22] ) or on microcavity resonance [23] have also been explored, delivering remarkable performance for applications requiring a particularly narrow spectral response. With respect to the NBA strategy, it builds on the inherent spectral tunability and narrowband absorption of many organic semiconductors. [24][25][26][27][28] In particular, NBA photo detectors are inherently nonfiltered, i.e., they have little/negligible impact on the spectral content of the incident light outside the target spectral range. Consequently, NBA photodetectors can potentially deliver multicolor/multispectral detection in a stacked configuration, [29][30][31] a route that is particularly attractive for high-resolution imaging beyond the capabilities of benchmark technologies. In fact, NBA photodetectors have been particularly successful in delivering narrowband functionality in the blue, green, and red spectral regions, attaining spectral responsivity passbands with typical spectral widths of ≈100-150 nm, and specific detectivity values up to the 10 13 Jones range. [11] Developing and emerging applications (e.g., requiring multispectral capability) motivate the effort to expand the scope of organic NBA narrowband photodetectors beyond the boundaries of conventional color detection. In particular, narrowband photodetection in the far-red range (i.e., photodetectors with peak response at 700-750 nm)-dimly visible to the human eye and at the same time distinct from the infrared regionis of considerable interest, in view of its role in many natural Spectrally-selective photodetection via organic semiconductors manifesting narrowband absorption (NBA photodetection) is highly attractive for emerging applications that require ultrathin, lightweight, and low-cost solutions. While successful over mainstream color bands, NBA photodetectors have struggled so far to meet the functional and/or performance demands at longer wavelengths, importantly in the far-red (700-750 nm), a range relevant to diverse applications in analytical biology, medical diagnostics, remote sensing, etc. In consideration of the potential of a nonfullerene-acceptor route to address this challenge, the narrowband photodetection capabilities of SBDTIC, ...

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Real-time, continuous, fluorescence sensing in a freely-moving subject with an implanted hybrid VCSEL/CMOS biosensor

Cited by 15 publications

References 17 publications

Miniaturized optical neuroimaging in unrestrained animals

Miniaturized optical neuroimaging in unrestrained animals

Overview of CMOS image sensor use in molecular diagnostics

Narrowband‐Absorption‐Type Organic Photodetectors for the Far‐Red Range Based on Fullerene‐Free Bulk Heterojunctions

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