Real-time damage monitoring of irradiated DNA

Abstract

This article presents a microfluidic technique for the real-time analysis of DNA damage due to radiation exposure. A continuous-flow spatial melting analysis was performed every three seconds on a sample of isolated DNA while it was being irradiated. The formation of photoproducts being caused by the UV-C radiation was monitored during the process. Cumulative damage produced distinct changes in the DNA melting curves, characterized by a shifting and broadening of the melting peaks. The design of the microfluidic device, the experimental procedure, and the analysis algorithm and interactive GUI are discussed herein. In addition, the advantages of this system are correlated to specific needs of related scientific studies, such as the investigation of sequence-specific damage susceptibility and the characterization of exposure-damage nonlinearities.

Advances in Microfluidics and Lab-on-a-Chip Technologies

“…Real-time damage monitoring of irradiated DNA (Pješčić, et al, 2011) The authors demonstrate real-time measurement of DNA damage due to radiation exposure using a microfluidic HRMA Genotyping from saliva with a one-step microdevice (Pješčić & Crews, 2012) PCR and spatial HRMA are carried out on a single disposable microfluidic chip and shown to distinguish between human male and female saliva samples. Table 2.…”