Real-time detection of Fe{middle dot}EDTA/H2O2-induced DNA cleavage by linear dichroism

Wang, Wei; Lee, Gil Jun; Jang, Kyeung Joo; Cho, Tae Sub; Kim, Seog K.

doi:10.1093/nar/gkn370

Cited by 17 publications

(19 citation statements)

References 30 publications

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“…Linear dichroism (LD) is defined by the difference in the absorption of polarized parallel and perpendicular radiation relative to the laboratory reference axis of the oriented sample [39][40][41]. The use of LD as a tool for detecting dsDNA cleavage in real-time is described elsewhere [19,[33][34][35]39].…”

Section: Ld and Other Spectroscopic Measurementsmentioning

confidence: 99%

“…The use of LD as a tool for detecting dsDNA cleavage in real-time is described elsewhere [19,[33][34][35]39]. The electrochemical measurements were conducted using an Ag/AgCl reference electrode, coiled platinum counter electrode and glassy carbon electrode (Bioanalytical Systems Inc., A = 0.071 cm 2 ).…”

Section: Ld and Other Spectroscopic Measurementsmentioning

confidence: 99%

“…The cleavage of both strands at a similar place shortens the DNA molecule, which also results in a lower LD magnitude in the DNA absorption region. Using this concept, LD is an excellent tool for probing the cleavage of sc and dsDNA in real-time[19,[33][34][35]39,[43][44][45][46].…”

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confidence: 99%

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Oxidative DNA cleavage by Cu(II) complexes: Effect of periphery substituent groups

Wang

Lee

Kim

et al. 2015

Journal of Inorganic Biochemistry

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Section: Ld and Other Spectroscopic Measurementsmentioning

confidence: 99%

Section: Ld and Other Spectroscopic Measurementsmentioning

confidence: 99%

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Oxidative DNA cleavage by Cu(II) complexes: Effect of periphery substituent groups

Wang

Lee

Kim

et al. 2015

Journal of Inorganic Biochemistry

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“…[41][42][43][44] Here, we report the synthesis and characterization of various transition metal complexes containing a dipyridylamine-based ligand bearing the anthracene moiety (Fig. [41][42][43][44] Here, we report the synthesis and characterization of various transition metal complexes containing a dipyridylamine-based ligand bearing the anthracene moiety (Fig.…”

Section: Introductionmentioning

confidence: 99%

“…[41][42][43][44] Here, we report the synthesis and characterization of various transition metal complexes containing a dipyridylamine-based ligand bearing the anthracene moiety (Fig. The experimental results showed that they can induce considerable oxidative DNA cleavage in the presence hydrogen peroxide and dioxygen.…”

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Synthesis, DNA binding profile and DNA cleavage pathway of divalent metal complexes

et al. 2015

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Graphical AbstractDivalent metal complexes of dipyridylamine ligand with an anthracene moiety induced considerable oxidative DNA cleavage in the presence hydrogen peroxide and dioxygen. AbstractComplexes of dipyridylamine based ligand with an anthracene moiety containing divalent metal ions Co(II), Cu(II), Ni(II), Zn(II) and Cd(II) were characterized structurally. The experimental results showed that they can induce considerable oxidative DNA cleavage in the presence hydrogen peroxide and dioxygen. The Zn(II) complex did not show any appreciable cleavage activity, whereas the Cd(II) and Ni(II) complexes were moderately active. On the other hand, Cu(II) and Co(II) complex showed the formation of a significant quantity of linear DNA resulting from the double-strand breaks. Mechanistic studies revealed the involvement of HO• and the superoxide anion to be the reactive species in the scission process in aerobic media. A mechanism involving either the Fenton or the Haber-Weiss reaction was proposed for the DNA cleavage mediated by these complexes. The Cu(II) complex could also cleave the double stranded calf thymus DNA (ds DNA) in the presence of activators, most likely via an oxidative mechanism, whereas the activity of the other complexes was negligible under similar reaction conditions. The kinetic aspects of ds DNA cleavage with the Cu(II) are detailed. The interaction of the five metal complexes with ds DNA was investigated by UV absorption and linear dichroism studies, and the mode of complexes binding to ds DNA is proposed. 4 species. 21,25-29 The chemistry of nucleic acid degradation by synthetic nucleases has been the subject of numerous studies. 18,30-36 On the other hand, only a limited number of reports have examined the actual rates of product formation (kinetics) during chemical nuclease induced DNA scissoring. 37-40 A recent development in the field of DNA cleavage are measurements of the reaction kinetics of the drug-mediated DNA cleavage by the linear dichroism (LD)technique. [41][42][43][44] Here, we report the synthesis and characterization of various transition metal complexes containing a dipyridylamine-based ligand bearing the anthracene moiety (Fig. 1).Anthracene moiety is one of fused aromatic hydrocarbons and is expected to intercalate between DNA base pair, thereby, enhance the binding efficiency to DNA. The ability of the complexes to promote the cleavage of two different DNA, namely, pBR322 plasmid DNA, and calf thymus DNA, was investigated. In the latter case, the detailed degradation kinetics were evaluated using the LD technique. The binding mode of the metal complexes to calf thymus DNA was also examined by absorption and LD spectroscopy.All chemicals were used as received unless stated otherwise. Calf thymus DNA (referred to as dsDNA) was purchased from Sigma and dissolved in a 5 mM cacodylate buffer (pH 7.0) containing 1 mM EDTA and 100 mM NaCl by gentle shaking at 4 °C. The dsDNA solution was dialyzed several times against 5 mM cacodylate buffer (pH 7.0) at 4 °C. The dsDNA concentrat...

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Effect of Various Intercalators on the Fenton‐Type Oxidative Cleavage of Double‐Stranded DNA

Kim

Sung

Kim

et al. 2014

Chemistry An Asian Journal

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The intensity of the linear dichroism (LD) in the absorption region of DNA (about 260 nm) decreased with time in the presence of [Fe(EDTA)](2+) (EDTA = ethylenediaminetetraacetic acid), H2O2, and ascorbate. The decrease in the LD signal indicated either an increase in flexibility, a shortening of the DNA stem, or both, owing to oxidative cleavage, and was best described by the difference between the two single-exponential-decay curves, thereby suggesting the involvement of two sequential first-order reactions. The fast reaction was assigned to cleavage of one of two DNA strands, which increased the flexibility of the DNA. The slow reaction corresponded to cleavage at or near the first cleavage site, thereby shortening the DNA stem. The presence of an intercalator, including ethidium, propidium, 9-aminoacridine, and proflavine, inhibited the first step of the cleavage reaction. One of the possible reasons for the observed inhibition might be a change in the DNA conformation near the intercalation site. Intercalation caused an unwinding and elongation of the DNA and resulted in changes in the location of the H atoms of the sugar moiety, which is known to be the main site at which hydroxyl radicals react.

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Real-time detection of Fe{middle dot}EDTA/H2O2-induced DNA cleavage by linear dichroism

Cited by 17 publications

References 30 publications

Oxidative DNA cleavage by Cu(II) complexes: Effect of periphery substituent groups

Oxidative DNA cleavage by Cu(II) complexes: Effect of periphery substituent groups

Synthesis, DNA binding profile and DNA cleavage pathway of divalent metal complexes

Effect of Various Intercalators on the Fenton‐Type Oxidative Cleavage of Double‐Stranded DNA

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