Micro- and nanorobots (MNRs) have attracted significant interest owing to their promising applications in various fields, including environmental monitoring, biomedicine, and microengineering. This review explores advances in the synthetic routes used for the preparation of MNRs, focusing on both top-down and bottom-up approaches. Although the top-down approach dominates the field because of its versatility in design and functionality, bottom-up strategies that utilize template-assisted electrochemical deposition and bioconjugation present unique advantages in terms of biocompatibility. This review investigates the diverse propulsion mechanisms employed in MNRs, including magnetic, electric, light, and biological forces, which enable efficient navigation in various fluidic environments. The interplay between the synthesis and propulsion mechanisms of MNRs in the development of colorimetric and fluorescence detection platforms is emphasized. Additionally, we summarize the recent advancements in MNRs as sensing and biosensing platforms, particularly focusing on colorimetric and fluorescence-based detection systems. By utilizing the controlled motion of MNRs, dynamic changes in the fluorescent signals and colorimetric responses can be achieved, thereby enhancing the sensitivity and selectivity of biomolecular detection. This review highlights the transformative potential of MNRs in sensing applications and emphasizes their role in advancing diagnostic technologies through innovative motion-driven signal transduction mechanisms. Subsequently, we provide an overview of the primary challenges currently faced in MNR research, along with our perspective on the future applications of MNR-assisted colorimetric and fluorescence biosensing in chemical and biological sensing. Moreover, issues related to enhanced stability, biocompatibility, and integration with existing detection systems are discussed.
